Novel Förster Resonance Energy Transfer probe with quantum dot
for a long-time imaging of active caspases inside individual
cells

J 2023

Novel Förster Resonance Energy Transfer probe with quantum dot for a long-time imaging of active caspases inside individual cells

PROCHÁZKOVÁ, Markéta, Eliška KUCHOVSKÁ, Michael KILLINGER a Karel KLEPÁRNÍK

Základní údaje

Originální název

Novel Förster Resonance Energy Transfer probe with quantum dot for a long-time imaging of active caspases inside individual cells

Autoři

PROCHÁZKOVÁ, Markéta (203 Česká republika, domácí), Eliška KUCHOVSKÁ (203 Česká republika), Michael KILLINGER (203 Česká republika, domácí) a Karel KLEPÁRNÍK (203 Česká republika, garant)

Vydání

Analytica Chimica Acta, Elsevier B.V. 2023, 0003-2670

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.200 v roce 2022

Kód RIV

RIV/00216224:14310/23:00130766

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.aca.2023.341334

UT WoS

001009667500001

Klíčová slova anglicky

Caspase molecular probe; Time-laps fluorescence microscopy; Quantum dot photostability; FRET probe

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 30. 1. 2024 20:27, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

With the goal to investigate biological phenomena at a single-cell level, we designed, synthesized and tested a molecular probe based on Förster resonance energy transfer (FRET) between a highly luminescent quantum dot (QD) as a donor and a fluorophore or fluorescence quencher as an acceptor linked by a specific peptide. In principle, QD luminescence, effectively dissipated in the probe, is switched on after the cleavage of the peptide by a protease and the release of the quencher. We proposed a novel synthesis strategy of a probe. A two-step synthesis consists of: (i) Conjugation of CdTe QDs functionalized by –COOH groups of succinic acid on the nanoparticle surface with the designed specific peptide (GTADVEDTSC) using a ligand-exchange approach; (ii) A fast, high-yield reaction of amine-reactive succinimidyl group on the BHQ-2 quencher with N-terminal of the peptide.

Návaznosti

MUNI/A/1421/2022, interní kód MU

Název: Vývoj metod a instrumentace pro analýzu biologicky významných látek 2023

Investor: Masarykova univerzita, Vývoj metod a instrumentace pro analýzu biologicky významných látek 2023

Citovat

PROCHÁZKOVÁ, Markéta, Eliška KUCHOVSKÁ, Michael KILLINGER a Karel KLEPÁRNÍK. Novel Förster Resonance Energy Transfer probe with quantum dot for a long-time imaging of active caspases inside individual cells. Analytica Chimica Acta. Elsevier B.V., 2023, roč. 1267, August, s. 1-9. ISSN 0003-2670. Dostupné z: https://dx.doi.org/10.1016/j.aca.2023.341334.

@article{2282018,
   author = {Procházková, Markéta and Kuchovská, Eliška and Killinger, Michael and Klepárník, Karel},
   article_number = {August},
   doi = {http://dx.doi.org/10.1016/j.aca.2023.341334},
   keywords = {Caspase molecular probe; Time-laps fluorescence microscopy; Quantum dot photostability; FRET probe},
   language = {eng},
   issn = {0003-2670},
   journal = {Analytica Chimica Acta},
   title = {Novel Förster Resonance Energy Transfer probe with quantum dot for a long-time imaging of active caspases inside individual cells},
   url = {https://www.sciencedirect.com/science/article/pii/S000326702300555X},
   volume = {1267},
   year = {2023}
}

TY  - JOUR
ID  - 2282018
AU  - Procházková, Markéta - Kuchovská, Eliška - Killinger, Michael - Klepárník, Karel
PY  - 2023
TI  - Novel Förster Resonance Energy Transfer probe with quantum dot for a long-time imaging of active caspases inside individual cells
JF  - Analytica Chimica Acta
VL  - 1267
IS  - August
SP  - 1-9
EP  - 1-9
PB  - Elsevier B.V.
SN  - 00032670
KW  - Caspase molecular probe
KW  - Time-laps fluorescence microscopy
KW  - Quantum dot photostability
KW  - FRET probe
UR  - https://www.sciencedirect.com/science/article/pii/S000326702300555X
N2  - With the goal to investigate biological phenomena at a single-cell level, we designed, synthesized and tested a molecular probe based on Förster resonance energy transfer (FRET) between a highly luminescent quantum dot (QD) as a donor and a fluorophore or fluorescence quencher as an acceptor linked by a specific peptide. In principle, QD luminescence, effectively dissipated in the probe, is switched on after the cleavage of the peptide by a protease and the release of the quencher. We proposed a novel synthesis strategy of a probe. A two-step synthesis consists of: (i) Conjugation of CdTe QDs functionalized by –COOH groups of succinic acid on the nanoparticle surface with the designed specific peptide (GTADVEDTSC) using a ligand-exchange approach; (ii) A fast, high-yield reaction of amine-reactive succinimidyl group on the BHQ-2 quencher with N-terminal of the peptide.
ER  -

PROCHÁZKOVÁ, Markéta, Eliška KUCHOVSKÁ, Michael KILLINGER a Karel KLEPÁRNÍK. Novel Förster Resonance Energy Transfer probe with quantum dot for a long-time imaging of active caspases inside individual cells. \textit{Analytica Chimica Acta}. Elsevier B.V., 2023, roč.~1267, August, s.~1-9. ISSN~0003-2670. Dostupné z: https://dx.doi.org/10.1016/j.aca.2023.341334.

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