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@article{2282018,
author = {Procházková, Markéta and Kuchovská, Eliška and Killinger, Michael and Klepárník, Karel},
article_number = {August},
doi = {http://dx.doi.org/10.1016/j.aca.2023.341334},
keywords = {Caspase molecular probe; Time-laps fluorescence microscopy; Quantum dot photostability; FRET probe},
language = {eng},
issn = {0003-2670},
journal = {Analytica Chimica Acta},
title = {Novel Förster Resonance Energy Transfer probe with quantum dot for a long-time imaging of active caspases inside individual cells},
url = {https://www.sciencedirect.com/science/article/pii/S000326702300555X},
volume = {1267},
year = {2023}
}
TY - JOUR
ID - 2282018
AU - Procházková, Markéta - Kuchovská, Eliška - Killinger, Michael - Klepárník, Karel
PY - 2023
TI - Novel Förster Resonance Energy Transfer probe with quantum dot for a long-time imaging of active caspases inside individual cells
JF - Analytica Chimica Acta
VL - 1267
IS - August
SP - 1-9
EP - 1-9
PB - Elsevier B.V.
SN - 00032670
KW - Caspase molecular probe
KW - Time-laps fluorescence microscopy
KW - Quantum dot photostability
KW - FRET probe
UR - https://www.sciencedirect.com/science/article/pii/S000326702300555X
N2 - With the goal to investigate biological phenomena at a single-cell level, we designed, synthesized and tested a molecular probe based on Förster resonance energy transfer (FRET) between a highly luminescent quantum dot (QD) as a donor and a fluorophore or fluorescence quencher as an acceptor linked by a specific peptide. In principle, QD luminescence, effectively dissipated in the probe, is switched on after the cleavage of the peptide by a protease and the release of the quencher. We proposed a novel synthesis strategy of a probe. A two-step synthesis consists of: (i) Conjugation of CdTe QDs functionalized by –COOH groups of succinic acid on the nanoparticle surface with the designed specific peptide (GTADVEDTSC) using a ligand-exchange approach; (ii) A fast, high-yield reaction of amine-reactive succinimidyl group on the BHQ-2 quencher with N-terminal of the peptide.
ER -
PROCHÁZKOVÁ, Markéta, Eliška KUCHOVSKÁ, Michael KILLINGER a Karel KLEPÁRNÍK. Novel Förster Resonance Energy Transfer probe with quantum dot for a long-time imaging of active caspases inside individual cells. \textit{Analytica Chimica Acta}. Elsevier B.V., 2023, roč.~1267, August, s.~1-9. ISSN~0003-2670. Dostupné z: https://dx.doi.org/10.1016/j.aca.2023.341334.
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