Evolution of genome size and GC content in the tribe Carduinae
(Asteraceae): rare descending dysploidy and polyploidy, limited
environmental control and strong phylogenetic signal

J 2023

Evolution of genome size and GC content in the tribe Carduinae (Asteraceae): rare descending dysploidy and polyploidy, limited environmental control and strong phylogenetic signal

BUREŠ, Petr, Melahat OZCAN, Jakub ŠMERDA, Ester MICHÁLKOVÁ, Lucie HOROVÁ et. al.

Základní údaje

Originální název

Evolution of genome size and GC content in the tribe Carduinae (Asteraceae): rare descending dysploidy and polyploidy, limited environmental control and strong phylogenetic signal

Název česky

Evoluce velikosti genomu a obsahu GC bází v tribu Carduinae: vzácná sestupná dysploidie a polyploidie, slabá kontrola prostředím a silný fylogenetický signál

Autoři

BUREŠ, Petr (203 Česká republika, garant, domácí), Melahat OZCAN, Jakub ŠMERDA (203 Česká republika, domácí), Ester MICHÁLKOVÁ (203 Česká republika, domácí), Lucie HOROVÁ (203 Česká republika, domácí), Klára PLAČKOVÁ (203 Česká republika, domácí), Petr ŠMARDA (203 Česká republika, domácí), Tammy L ELLIOTT (124 Kanada, domácí), Pavel VESELÝ (203 Česká republika, domácí), Sebastian ĆATO, Maryam NOROUZI, Masoud SHEIDAI a František ZEDEK (203 Česká republika, domácí)

Vydání

Preslia, Czech Botanical Society, 2023, 0032-7786

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.400 v roce 2022

Kód RIV

RIV/00216224:14310/23:00130776

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.23855/PRESLIA.2023.185

UT WoS

000976524400004

Klíčová slova anglicky

achene size; flow cytometry; genomic DNA base composition; guard cell length; nuclear DNA content; phylogeny

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 6. 1. 2024 00:19, prof. RNDr. Petr Bureš, Ph.D.

Anotace

V originále

Genome size and GC content are basic species-specific attributes often delimiting genera or higher taxa, which enable the identification of polyploidy, hybridization and other modes of genome or karyotype evolution. The evolution of these genomic traits can often occur as a result of the selective pressure of the environment. Here, we reconstruct the evolution of these genomic traits in subtribe Carduinae (Asteraceae) in the context of changes in chromosome numbers. Using flow cytometry, genome size and GC content were estimated for 119 taxa and mapped onto a phylogenetic tree constructed using sequences from seven genetic markers. In addition, the genomic data were compared with the length of stomatal guard cells and achene size (length, weight) to evaluate the extent to which genomic characters could evolve adaptively in this subtribe. We found strong phylogenetic signals for the analysed genomic and phenotypic traits, which delimited most Carduinae genera or clades in agreement with the reconstructed phylogeny. Monoploid genome size was positively correlated with genomic GC content and stomatal guard cell length. In Cirsium, whose species were the focus of the majority of the analyses, the large-genomed subgen. Lophiolepis had smaller guard cells, which might be related to it occurring in more xeric habitats compared to subgen. Cirsium. In contrast, the achenes of the large-genomed subgen. Lophiolepis were larger, possibly in response to the summer drought, whereas achene weight and length were independent of genome size across the subtribe. Thus, genome size in the subtribe Carduinae might evolve under weak environmental control, at least under that mediated by the size of guard cells or achenes. Achene size was related positively to GC content, which could have evolved adaptively in response to summer drought. In Carduus and the North American Cirsium taxa, there is an increase in average chromosome size with reduction in monoploid chromosome number, suggesting descending dysploidy associated with chromosomal fusion. Polyploidy is relatively rare in this subtribe and was confirmed only in five of the species studied, including Cirsium vulgare, an invasive species that likely originated via distant (intersubgeneric) hybridization, as suggested by its intermediate genomic and achene features combined with the conflict between its morphology and phylogenetic position. Phylogenetic reconstruction, differences in genomic parameters, as well as stomatal guard cell and achene sizes support the separation of a monophyletic Lophiolepis from the remainder of Cirsium. Genome and achene size results also indicate that the early diverging Cirsium italicum can be separated from the rest of the monophyletic Lophiolepis+Picnomon+Notobasis clade.

Návaznosti

GA20-15989S, projekt VaV

Název: Evoluce velikosti genomu - centromerický drajv v nové roli (Akronym: Centrogenomtah)

Investor: Grantová agentura ČR, Evolution of genome size - a new role for the centromere drive

LM2018131, projekt VaV

Název: Česká národní infrastruktura pro biologická data (Akronym: ELIXIR-CZ)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Česká národní infrastruktura pro biologická data

LM2018140, projekt VaV

Název: e-Infrastruktura CZ (Akronym: e-INFRA CZ)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, e-Infrastruktura CZ

Citovat

BUREŠ, Petr, Melahat OZCAN, Jakub ŠMERDA, Ester MICHÁLKOVÁ, Lucie HOROVÁ, Klára PLAČKOVÁ, Petr ŠMARDA, Tammy L ELLIOTT, Pavel VESELÝ, Sebastian ĆATO, Maryam NOROUZI, Masoud SHEIDAI a František ZEDEK. Evolution of genome size and GC content in the tribe Carduinae (Asteraceae): rare descending dysploidy and polyploidy, limited environmental control and strong phylogenetic signal. Preslia. Czech Botanical Society, 2023, roč. 95, č. 1, s. 185-213. ISSN 0032-7786. Dostupné z: https://dx.doi.org/10.23855/PRESLIA.2023.185.

@article{2282737,
   author = {Bureš, Petr and Ozcan, Melahat and Šmerda, Jakub and Michálková, Ester and Horová, Lucie and Plačková, Klára and Šmarda, Petr and Elliott, Tammy L and Veselý, Pavel and Ćato, Sebastian and Norouzi, Maryam and Sheidai, Masoud and Zedek, František},
   article_number = {1},
   doi = {http://dx.doi.org/10.23855/PRESLIA.2023.185},
   keywords = {achene size; flow cytometry; genomic DNA base composition; guard cell length; nuclear DNA content; phylogeny},
   language = {eng},
   issn = {0032-7786},
   journal = {Preslia},
   title = {Evolution of genome size and GC content in the tribe Carduinae (Asteraceae): rare descending dysploidy and polyploidy, limited environmental control and strong phylogenetic signal},
   url = {https://doi.org/10.23855/preslia.2023.185},
   volume = {95},
   year = {2023}
}

TY  - JOUR
ID  - 2282737
AU  - Bureš, Petr - Ozcan, Melahat - Šmerda, Jakub - Michálková, Ester - Horová, Lucie - Plačková, Klára - Šmarda, Petr - Elliott, Tammy L - Veselý, Pavel - Ćato, Sebastian - Norouzi, Maryam - Sheidai, Masoud - Zedek, František
PY  - 2023
TI  - Evolution of genome size and GC content in the tribe Carduinae (Asteraceae): rare descending dysploidy and polyploidy, limited environmental control and strong phylogenetic signal
JF  - Preslia
VL  - 95
IS  - 1
SP  - 185-213
EP  - 185-213
PB  - Czech Botanical Society
SN  - 00327786
KW  - achene size
KW  - flow cytometry
KW  - genomic DNA base composition
KW  - guard cell length
KW  - nuclear DNA content
KW  - phylogeny
UR  - https://doi.org/10.23855/preslia.2023.185
N2  - Genome size and GC content are basic species-specific attributes often delimiting genera or higher taxa, which enable the identification of polyploidy, hybridization and other modes of genome or karyotype evolution. The evolution of these genomic traits can often occur as a result of the selective pressure of the environment. Here, we reconstruct the evolution of these genomic traits in subtribe Carduinae (Asteraceae) in the context of changes in chromosome numbers. Using flow cytometry, genome size and GC content were estimated for 119 taxa and mapped onto a phylogenetic tree constructed using sequences from seven genetic markers. In addition, the genomic data were compared with the length of stomatal guard cells and achene size (length, weight) to evaluate the extent to which genomic characters could evolve adaptively in this subtribe. We found strong phylogenetic signals for the analysed genomic and phenotypic traits, which delimited most Carduinae genera or clades in agreement with the reconstructed phylogeny. Monoploid genome size was positively correlated with genomic GC content and stomatal guard cell length. In Cirsium, whose species were the focus of the majority of the analyses, the large-genomed subgen. Lophiolepis had smaller guard cells, which might be related to it occurring in more xeric habitats compared to subgen. Cirsium. In contrast, the achenes of the large-genomed subgen. Lophiolepis were larger, possibly in response to the summer drought, whereas achene weight and length were independent of genome size across the subtribe. Thus, genome size in the subtribe Carduinae might evolve under weak environmental control, at least under that mediated by the size of guard cells or achenes. Achene size was related positively to GC content, which could have evolved adaptively in response to summer drought. In Carduus and the North American Cirsium taxa, there is an increase in average chromosome size with reduction in monoploid chromosome number, suggesting descending dysploidy associated with chromosomal fusion. Polyploidy is relatively rare in this subtribe and was confirmed only in five of the species studied, including Cirsium vulgare, an invasive species that likely originated via distant (intersubgeneric) hybridization, as suggested by its intermediate genomic and achene features combined with the conflict between its morphology and phylogenetic position. Phylogenetic reconstruction, differences in genomic parameters, as well as stomatal guard cell and achene sizes support the separation of a monophyletic Lophiolepis from the remainder of Cirsium. Genome and achene size results also indicate that the early diverging Cirsium italicum can be separated from the rest of the monophyletic Lophiolepis+Picnomon+Notobasis clade.
ER  -

BUREŠ, Petr, Melahat OZCAN, Jakub ŠMERDA, Ester MICHÁLKOVÁ, Lucie HOROVÁ, Klára PLAČKOVÁ, Petr ŠMARDA, Tammy L ELLIOTT, Pavel VESELÝ, Sebastian $\backslash$'CATO, Maryam NOROUZI, Masoud SHEIDAI a František ZEDEK. Evolution of genome size and GC content in the tribe Carduinae (Asteraceae): rare descending dysploidy and polyploidy, limited environmental control and strong phylogenetic signal. \textit{Preslia}. Czech Botanical Society, 2023, roč.~95, č.~1, s.~185-213. ISSN~0032-7786. Dostupné z: https://dx.doi.org/10.23855/PRESLIA.2023.185.

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