J 2023

Comprehensive analysis of floral scent and fatty acids in nectar of Silene nutans through modern analytical gas chromatography techniques

SPADAFORA, Natasha Damiana, Damien EGGERMONT, Veronika KŘEŠŤÁKOVÁ, Tatiana CHENET, Fabienne VAN ROSSUM et. al.

Základní údaje

Originální název

Comprehensive analysis of floral scent and fatty acids in nectar of Silene nutans through modern analytical gas chromatography techniques

Autoři

SPADAFORA, Natasha Damiana, Damien EGGERMONT, Veronika KŘEŠŤÁKOVÁ (203 Česká republika, domácí), Tatiana CHENET, Fabienne VAN ROSSUM a Giorgia PURCARO (garant)

Vydání

Journal of Chromatography A, Elsevier, 2023, 0021-9673

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Impakt faktor

Impact factor: 4.100 v roce 2022

Kód RIV

RIV/00216224:14310/23:00130780

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000982832600001

Klíčová slova anglicky

Head-space extraction; Chemometrics; GC × GC; Fatty acids; Volatile organic compounds

Štítky

Příznaky

Mezinárodní význam, Recenzováno
Změněno: 2. 6. 2023 14:53, Mgr. Marie Šípková, DiS.

Anotace

V originále

The aim of this work was to show the potential of multidimensional gas chromatography combined with mass spectrometry and suitable chemometrics means based on untargeted and profiling data analysis to strengthen the information provided by floral scent and nectar fatty acids of four genetically differentiated lineages (E1, W1, W2, and W3) of the nocturnal moth-pollinated herb Silene nutans. Volatile organic compounds emitted by flowers were trapped for a total of 42 samples by in-vivo sampling dynamic head space for analysing floral scent by untargeted approach, while 37 samples of nectar were collected for analysing fatty acids through profiling analysis. The resulting data from floral scent analysis were aligned and compared using a tile-based methodology followed by data mining to access high-level information. Based on floral scent and nectar fatty acid results, it was possible to distinguish E1 from the W lineages, and W3 from W1 and W2. This work puts the bases for a larger study aiming to clarify the existence of prezygotic barriers involved in speciation among lineages of S. nutans, and thus the possible implication of different flower scents and nectar compositions in this phenomenon.