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@article{2285657,
author = {Nováková, Dana and Koublová, Vendula and Sedlář, Karel and Staňková, Eva and Králová, Stanislava and Švec, Pavel and NeumannandSchaal, Meina and Wolf, Jacqueline and Koudelková, Sylva and Barták, Miloš and Sedláček, Ivo},
article_number = {4},
doi = {http://dx.doi.org/10.1016/j.syapm.2023.126424},
keywords = {Pseudomonas; P. petrae sp. nov.; Antarctica; Regolith; Species description; Taxonomy},
language = {eng},
issn = {0723-2020},
journal = {Systematic and Applied Microbiology},
title = {Pseudomonas petrae sp. nov. isolated from regolith samples in Antarctica},
url = {https://doi.org/10.1016/j.syapm.2023.126424},
volume = {46},
year = {2023}
}
TY - JOUR
ID - 2285657
AU - Nováková, Dana - Koublová, Vendula - Sedlář, Karel - Staňková, Eva - Králová, Stanislava - Švec, Pavel - Neumann-Schaal, Meina - Wolf, Jacqueline - Koudelková, Sylva - Barták, Miloš - Sedláček, Ivo
PY - 2023
TI - Pseudomonas petrae sp. nov. isolated from regolith samples in Antarctica
JF - Systematic and Applied Microbiology
VL - 46
IS - 4
SP - 1-9
EP - 1-9
PB - Elsevier GmbH
SN - 07232020
KW - Pseudomonas
KW - P. petrae sp. nov.
KW - Antarctica
KW - Regolith
KW - Species description
KW - Taxonomy
UR - https://doi.org/10.1016/j.syapm.2023.126424
N2 - A polyphasic taxonomic approach was used to characterize the four strains P2653T, P2652, P2498, and P2647, isolated from Antarctic regolith samples. Initial genotype screening performed by PCR fingerprinting based on repetitive sequences showed that the isolates studied formed a coherent cluster separated from the other Pseudomonas species. Identification results based on 16S rRNA gene sequences showed the highest sequence similarity with Pseudomonas graminis (99.7%), which was confirmed by multilocus sequence analysis using the rpoB, rpoD, and gyrB genes. Genome sequence comparison of P2653T with the most related P. graminis type strain DSM 11363T revealed an average nucleotide identity of 92.1% and a digital DNA-DNA hybridization value of 46.6%. The major fatty acids for all Antarctic strains were C16:0, Summed Feature 3 (C16:1 ω7c/C16:1 ω6c) and Summed Feature 8 (C18:1 ω7c/C18:1 ω6c). The predominant respiratory quinone was Q-9, and the major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, and phosphatidylglycerol. The regolith strains could be differentiated from related species by the absence of arginine dihydrolase, ornithine and lysine decarboxylase and by negative tyrosine hydrolysis. The results of this polyphasic study allowed the genotypic and phenotypic differentiation of four analysed strains from the closest related species, which confirmed that the strains represent a novel species within the genus Pseudomonas, for which the name Pseudomonas petrae sp. nov. is proposed with P2653T (CCM 8850T = DSM 112068T = LMG 30619T) as the type strain.
ER -
NOVÁKOVÁ, Dana, Vendula KOUBLOVÁ, Karel SEDLÁŘ, Eva STAŇKOVÁ, Stanislava KRÁLOVÁ, Pavel ŠVEC, Meina NEUMANN-SCHAAL, Jacqueline WOLF, Sylva KOUDELKOVÁ, Miloš BARTÁK a Ivo SEDLÁČEK. Pseudomonas petrae sp. nov. isolated from regolith samples in Antarctica. \textit{Systematic and Applied Microbiology}. Elsevier GmbH, 2023, roč.~46, č.~4, s.~1-9. ISSN~0723-2020. Dostupné z: https://dx.doi.org/10.1016/j.syapm.2023.126424.
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