Pseudomonas petrae sp. nov. isolated from regolith samples in
Antarctica

J 2023

Pseudomonas petrae sp. nov. isolated from regolith samples in Antarctica

NOVÁKOVÁ, Dana; Vendula KOUBLOVÁ; Karel SEDLÁŘ; Eva STAŇKOVÁ; Stanislava KRÁLOVÁ et. al.

Základní údaje

Originální název

Pseudomonas petrae sp. nov. isolated from regolith samples in Antarctica

Autoři

NOVÁKOVÁ, Dana (203 Česká republika, garant, domácí); Vendula KOUBLOVÁ (203 Česká republika, domácí); Karel SEDLÁŘ; Eva STAŇKOVÁ (203 Česká republika, domácí); Stanislava KRÁLOVÁ (703 Slovensko, domácí); Pavel ŠVEC (203 Česká republika, domácí); Meina NEUMANN-SCHAAL; Jacqueline WOLF; Sylva KOUDELKOVÁ (203 Česká republika, domácí); Miloš BARTÁK (203 Česká republika, domácí) a Ivo SEDLÁČEK (203 Česká republika, domácí)

Vydání

Systematic and Applied Microbiology, Elsevier GmbH, 2023, 0723-2020

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.300

Kód RIV

RIV/00216224:14310/23:00130840

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.syapm.2023.126424

UT WoS

001001607000001

EID Scopus

2-s2.0-85158014315

Klíčová slova anglicky

Pseudomonas; P. petrae sp. nov.; Antarctica; Regolith; Species description; Taxonomy

Štítky

CF PROT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 8. 4. 2024 10:08, Mgr. Tereza Miškechová

Anotace

V originále

A polyphasic taxonomic approach was used to characterize the four strains P2653T, P2652, P2498, and P2647, isolated from Antarctic regolith samples. Initial genotype screening performed by PCR fingerprinting based on repetitive sequences showed that the isolates studied formed a coherent cluster separated from the other Pseudomonas species. Identification results based on 16S rRNA gene sequences showed the highest sequence similarity with Pseudomonas graminis (99.7%), which was confirmed by multilocus sequence analysis using the rpoB, rpoD, and gyrB genes. Genome sequence comparison of P2653T with the most related P. graminis type strain DSM 11363T revealed an average nucleotide identity of 92.1% and a digital DNA-DNA hybridization value of 46.6%. The major fatty acids for all Antarctic strains were C16:0, Summed Feature 3 (C16:1 ω7c/C16:1 ω6c) and Summed Feature 8 (C18:1 ω7c/C18:1 ω6c). The predominant respiratory quinone was Q-9, and the major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, and phosphatidylglycerol. The regolith strains could be differentiated from related species by the absence of arginine dihydrolase, ornithine and lysine decarboxylase and by negative tyrosine hydrolysis. The results of this polyphasic study allowed the genotypic and phenotypic differentiation of four analysed strains from the closest related species, which confirmed that the strains represent a novel species within the genus Pseudomonas, for which the name Pseudomonas petrae sp. nov. is proposed with P2653T (CCM 8850T = DSM 112068T = LMG 30619T) as the type strain.

Návaznosti

EF18_046/0015974, projekt VaV

Název: Modernizace České infrastruktury pro integrativní strukturní biologii

LM2015078, projekt VaV

Název: Česká polární výzkumná infrastruktura (Akronym: CzechPolar2)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Polar Research Infrastructure

LM2018127, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

90242, velká výzkumná infrastruktura

Název: CIISB III

Citovat

NOVÁKOVÁ, Dana; Vendula KOUBLOVÁ; Karel SEDLÁŘ; Eva STAŇKOVÁ; Stanislava KRÁLOVÁ; Pavel ŠVEC; Meina NEUMANN-SCHAAL; Jacqueline WOLF; Sylva KOUDELKOVÁ; Miloš BARTÁK a Ivo SEDLÁČEK. Pseudomonas petrae sp. nov. isolated from regolith samples in Antarctica. Systematic and Applied Microbiology. Elsevier GmbH, 2023, roč. 46, č. 4, s. 1-9. ISSN 0723-2020. Dostupné z: https://dx.doi.org/10.1016/j.syapm.2023.126424.

@article{2285657,
   author = {Nováková, Dana and Koublová, Vendula and Sedlář, Karel and Staňková, Eva and Králová, Stanislava and Švec, Pavel and NeumannandSchaal, Meina and Wolf, Jacqueline and Koudelková, Sylva and Barták, Miloš and Sedláček, Ivo},
   article_number = {4},
   doi = {http://dx.doi.org/10.1016/j.syapm.2023.126424},
   keywords = {Pseudomonas; P. petrae sp. nov.; Antarctica; Regolith; Species description; Taxonomy},
   language = {eng},
   issn = {0723-2020},
   journal = {Systematic and Applied Microbiology},
   title = {Pseudomonas petrae sp. nov. isolated from regolith samples in Antarctica},
   url = {https://doi.org/10.1016/j.syapm.2023.126424},
   volume = {46},
   year = {2023}
}

TY  - JOUR
ID  - 2285657
AU  - Nováková, Dana - Koublová, Vendula - Sedlář, Karel - Staňková, Eva - Králová, Stanislava - Švec, Pavel - Neumann-Schaal, Meina - Wolf, Jacqueline - Koudelková, Sylva - Barták, Miloš - Sedláček, Ivo
PY  - 2023
TI  - Pseudomonas petrae sp. nov. isolated from regolith samples in Antarctica
JF  - Systematic and Applied Microbiology
VL  - 46
IS  - 4
SP  - 1-9
EP  - 1-9
PB  - Elsevier GmbH
SN  - 07232020
KW  - Pseudomonas
KW  - P. petrae sp. nov.
KW  - Antarctica
KW  - Regolith
KW  - Species description
KW  - Taxonomy
UR  - https://doi.org/10.1016/j.syapm.2023.126424
N2  - A polyphasic taxonomic approach was used to characterize the four strains P2653T, P2652, P2498, and P2647, isolated from Antarctic regolith samples. Initial genotype screening performed by PCR fingerprinting based on repetitive sequences showed that the isolates studied formed a coherent cluster separated from the other Pseudomonas species. Identification results based on 16S rRNA gene sequences showed the highest sequence similarity with Pseudomonas graminis (99.7%), which was confirmed by multilocus sequence analysis using the rpoB, rpoD, and gyrB genes. Genome sequence comparison of P2653T with the most related P. graminis type strain DSM 11363T revealed an average nucleotide identity of 92.1% and a digital DNA-DNA hybridization value of 46.6%. The major fatty acids for all Antarctic strains were C16:0, Summed Feature 3 (C16:1 ω7c/C16:1 ω6c) and Summed Feature 8 (C18:1 ω7c/C18:1 ω6c). The predominant respiratory quinone was Q-9, and the major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, and phosphatidylglycerol. The regolith strains could be differentiated from related species by the absence of arginine dihydrolase, ornithine and lysine decarboxylase and by negative tyrosine hydrolysis. The results of this polyphasic study allowed the genotypic and phenotypic differentiation of four analysed strains from the closest related species, which confirmed that the strains represent a novel species within the genus Pseudomonas, for which the name Pseudomonas petrae sp. nov. is proposed with P2653T (CCM 8850T = DSM 112068T = LMG 30619T) as the type strain.
ER  -

NOVÁKOVÁ, Dana; Vendula KOUBLOVÁ; Karel SEDLÁŘ; Eva STAŇKOVÁ; Stanislava KRÁLOVÁ; Pavel ŠVEC; Meina NEUMANN-SCHAAL; Jacqueline WOLF; Sylva KOUDELKOVÁ; Miloš BARTÁK a Ivo SEDLÁČEK. Pseudomonas petrae sp. nov. isolated from regolith samples in Antarctica. \textit{Systematic and Applied Microbiology}. Elsevier GmbH, 2023, roč.~46, č.~4, s.~1-9. ISSN~0723-2020. Dostupné z: https://dx.doi.org/10.1016/j.syapm.2023.126424.

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