In-depth Temporal Transcriptome Profiling of Monkeypox and Host
Cells using Nanopore Sequencing

J 2023

In-depth Temporal Transcriptome Profiling of Monkeypox and Host Cells using Nanopore Sequencing

KAKUK, Balázs, Ákos DÖRMŐ, Zsolt CSABAI, Gábor KEMENESI, Jiří HOLOUBEK et. al.

Basic information

Original name

In-depth Temporal Transcriptome Profiling of Monkeypox and Host Cells using Nanopore Sequencing

Authors

KAKUK, Balázs, Ákos DÖRMŐ, Zsolt CSABAI, Gábor KEMENESI, Jiří HOLOUBEK (203 Czech Republic, belonging to the institution), Daniel RŮŽEK (203 Czech Republic, belonging to the institution), István PRAZSÁK, Virág Éva DANI, Béla DÉNES, Gábor TORMA, Ferenc JAKAB, Gábor E. TÓTH, Fanni V. FÖLDES, Brigitta ZANA, Zsófia LANSZKI, Ákos HARANGOZÓ, Ádám FÜLÖP, Gábor GULYÁS, Máté MIZIK, András Attila KISS, Dóra TOMBÁCZ and Zsolt BOLDOGKŐI (guarantor)

Edition

Scientific Data, Nature Publishing Group, 2023, 2052-4463

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 9.800 in 2022

RIV identification code

RIV/00216224:14310/23:00130853

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1038/s41597-023-02149-4

UT WoS

001001297200001

Keywords in English

Pox virus; Transcriptomics; Virus–host interactions; mpox; monkeypox; nanopore sequencing

Abstract

V originále

The recent human Monkeypox outbreak underlined the importance of studying basic biology of orthopoxviruses. However, the transcriptome of its causative agent has not been investigated before neither with short-, nor with long-read sequencing approaches. This Oxford Nanopore long-read RNA-Sequencing dataset fills this gap. It will enable the in-depth characterization of the transcriptomic architecture of the monkeypox virus, and may even make possible to annotate novel host transcripts. Moreover, our direct cDNA and native RNA sequencing reads will allow the estimation of gene expression changes of both the virus and the host cells during the infection. Overall, our study will lead to a deeper understanding of the alterations caused by the viral infection on a transcriptome level.

Citovat

KAKUK, Balázs, Ákos DÖRMŐ, Zsolt CSABAI, Gábor KEMENESI, Jiří HOLOUBEK, Daniel RŮŽEK, István PRAZSÁK, Virág Éva DANI, Béla DÉNES, Gábor TORMA, Ferenc JAKAB, Gábor E. TÓTH, Fanni V. FÖLDES, Brigitta ZANA, Zsófia LANSZKI, Ákos HARANGOZÓ, Ádám FÜLÖP, Gábor GULYÁS, Máté MIZIK, András Attila KISS, Dóra TOMBÁCZ and Zsolt BOLDOGKŐI. In-depth Temporal Transcriptome Profiling of Monkeypox and Host Cells using Nanopore Sequencing. Scientific Data. Nature Publishing Group, 2023, vol. 10, No 262, p. 1-12. ISSN 2052-4463. Available from: https://dx.doi.org/10.1038/s41597-023-02149-4.

@article{2286039,
   author = {Kakuk, Balázs and Dörmő, Ákos and Csabai, Zsolt and Kemenesi, Gábor and Holoubek, Jiří and Růžek, Daniel and Prazsák, István and Dani, Virág Éva and Dénes, Béla and Torma, Gábor and Jakab, Ferenc and Tóth, Gábor E. and Földes, Fanni V. and Zana, Brigitta and Lanszki, Zsófia and Harangozó, Ákos and Fülöp, Ádám and Gulyás, Gábor and Mizik, Máté and Kiss, András Attila and Tombácz, Dóra and Boldogkői, Zsolt},
   article_number = {262},
   doi = {http://dx.doi.org/10.1038/s41597-023-02149-4},
   keywords = {Pox virus; Transcriptomics; Virus–host interactions; mpox; monkeypox; nanopore sequencing},
   language = {eng},
   issn = {2052-4463},
   journal = {Scientific Data},
   title = {In-depth Temporal Transcriptome Profiling of Monkeypox and Host Cells using Nanopore Sequencing},
   url = {https://doi.org/10.1038/s41597-023-02149-4},
   volume = {10},
   year = {2023}
}

TY  - JOUR
ID  - 2286039
AU  - Kakuk, Balázs - Dörmő, Ákos - Csabai, Zsolt - Kemenesi, Gábor - Holoubek, Jiří - Růžek, Daniel - Prazsák, István - Dani, Virág Éva - Dénes, Béla - Torma, Gábor - Jakab, Ferenc - Tóth, Gábor E. - Földes, Fanni V. - Zana, Brigitta - Lanszki, Zsófia - Harangozó, Ákos - Fülöp, Ádám - Gulyás, Gábor - Mizik, Máté - Kiss, András Attila - Tombácz, Dóra - Boldogkői, Zsolt
PY  - 2023
TI  - In-depth Temporal Transcriptome Profiling of Monkeypox and Host Cells using Nanopore Sequencing
JF  - Scientific Data
VL  - 10
IS  - 262
SP  - 1-12
EP  - 1-12
PB  - Nature Publishing Group
SN  - 20524463
KW  - Pox virus
KW  - Transcriptomics
KW  - Virus–host interactions
KW  - mpox
KW  - monkeypox
KW  - nanopore sequencing
UR  - https://doi.org/10.1038/s41597-023-02149-4
N2  - The recent human Monkeypox outbreak underlined the importance of studying basic biology of orthopoxviruses. However, the transcriptome of its causative agent has not been investigated before neither with short-, nor with long-read sequencing approaches. This Oxford Nanopore long-read RNA-Sequencing dataset fills this gap. It will enable the in-depth characterization of the transcriptomic architecture of the monkeypox virus, and may even make possible to annotate novel host transcripts. Moreover, our direct cDNA and native RNA sequencing reads will allow the estimation of gene expression changes of both the virus and the host cells during the infection. Overall, our study will lead to a deeper understanding of the alterations caused by the viral infection on a transcriptome level.
ER  -

KAKUK, Balázs, Ákos DÖRM$\backslash$H O, Zsolt CSABAI, Gábor KEMENESI, Jiří HOLOUBEK, Daniel RŮŽEK, István PRAZSÁK, Virág Éva DANI, Béla DÉNES, Gábor TORMA, Ferenc JAKAB, Gábor E. TÓTH, Fanni V. FÖLDES, Brigitta ZANA, Zsófia LANSZKI, Ákos HARANGOZÓ, Ádám FÜLÖP, Gábor GULYÁS, Máté MIZIK, András Attila KISS, Dóra TOMBÁCZ and Zsolt BOLDOGK$\backslash$H OI. In-depth Temporal Transcriptome Profiling of Monkeypox and Host Cells using Nanopore Sequencing. \textit{Scientific Data}. Nature Publishing Group, 2023, vol.~10, No~262, p.~1-12. ISSN~2052-4463. Available from: https://dx.doi.org/10.1038/s41597-023-02149-4.

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