Do conformational changes contribute to the surface plasmon
resonance signal?

J 2023

Do conformational changes contribute to the surface plasmon resonance signal?

DOBROVODSKÝ, Daniel a Carmelo DI PRIMO

Základní údaje

Originální název

Do conformational changes contribute to the surface plasmon resonance signal?

Autoři

DOBROVODSKÝ, Daniel (703 Slovensko, domácí) a Carmelo DI PRIMO (250 Francie, garant)

Vydání

Biosensors and Bioelectronics, Elsevier, 2023, 0956-5663

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 12.600 v roce 2022

Kód RIV

RIV/00216224:14310/23:00130874

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.bios.2023.115296

UT WoS

000986118600001

Klíčová slova anglicky

Surface plasmon resonance; Conformational change; G-quadruplex; Refractive index increment; Thrombin binding aptamer

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 6. 2023 15:09, Mgr. Marie Šípková, DiS.

Anotace

V originále

Surface plasmon resonance (SPR)-based biosensors are widely used instruments for characterizing molecular interactions. In theory the SPR signal depends only on mass changes for interacting molecules of same chemical nature. Whether conformational changes of interacting molecules also contribute to the SPR signal is still a subject of lively debates. Works have been published claiming that conformational changes were detected but all factors contributing to the SPR signal were not carefully considered, in addition to often using no or improper controls. In the present work we used a very well-characterized oligonucleotide, the thrombin-binding DNA aptamer (TBA), which upon binding of potassium ions folds into a two G-tetrad antiparallel G-quadruplex structure. All terms contributing to the maximal expected SPR response, Rmax, in particular the refractive index increment, RII, of both partners and the fraction of immobilized TBA target available, ca, were experimentally assessed. The resulting Rmax was then compared to the maximal experimental SPR response for potassium ions binding to TBA using appropriate controls. Regardless how the RIIs were measured, by SPR or refractometry, and how much TBA available for interacting with potassium ions was considered, the theoretical and the experi-mental SPR responses never matched, the former being always lower than the latter. Using a straightforward experimental model system and by thoroughly taking into account all contributing factors we therefore conclude that conformational changes can indeed contribute to the measured SPR signal.

Citovat

DOBROVODSKÝ, Daniel a Carmelo DI PRIMO. Do conformational changes contribute to the surface plasmon resonance signal? Biosensors and Bioelectronics. Elsevier, 2023, roč. 232, July, s. 1-9. ISSN 0956-5663. Dostupné z: https://dx.doi.org/10.1016/j.bios.2023.115296.

@article{2287058,
   author = {Dobrovodský, Daniel and Di Primo, Carmelo},
   article_number = {July},
   doi = {http://dx.doi.org/10.1016/j.bios.2023.115296},
   keywords = {Surface plasmon resonance; Conformational change; G-quadruplex; Refractive index increment; Thrombin binding aptamer},
   language = {eng},
   issn = {0956-5663},
   journal = {Biosensors and Bioelectronics},
   title = {Do conformational changes contribute to the surface plasmon resonance signal?},
   url = {https://doi.org/10.1016/j.bios.2023.115296},
   volume = {232},
   year = {2023}
}

TY  - JOUR
ID  - 2287058
AU  - Dobrovodský, Daniel - Di Primo, Carmelo
PY  - 2023
TI  - Do conformational changes contribute to the surface plasmon resonance signal?
JF  - Biosensors and Bioelectronics
VL  - 232
IS  - July
SP  - 1-9
EP  - 1-9
PB  - Elsevier
SN  - 09565663
KW  - Surface plasmon resonance
KW  - Conformational change
KW  - G-quadruplex
KW  - Refractive index increment
KW  - Thrombin binding aptamer
UR  - https://doi.org/10.1016/j.bios.2023.115296
N2  - Surface plasmon resonance (SPR)-based biosensors are widely used instruments for characterizing molecular interactions. In theory the SPR signal depends only on mass changes for interacting molecules of same chemical nature. Whether conformational changes of interacting molecules also contribute to the SPR signal is still a subject of lively debates. Works have been published claiming that conformational changes were detected but all factors contributing to the SPR signal were not carefully considered, in addition to often using no or improper controls. In the present work we used a very well-characterized oligonucleotide, the thrombin-binding DNA aptamer (TBA), which upon binding of potassium ions folds into a two G-tetrad antiparallel G-quadruplex structure. All terms contributing to the maximal expected SPR response, Rmax, in particular the refractive index increment, RII, of both partners and the fraction of immobilized TBA target available, ca, were experimentally assessed. The resulting Rmax was then compared to the maximal experimental SPR response for potassium ions binding to TBA using appropriate controls. Regardless how the RIIs were measured, by SPR or refractometry, and how much TBA available for interacting with potassium ions was considered, the theoretical and the experi-mental SPR responses never matched, the former being always lower than the latter. Using a straightforward experimental model system and by thoroughly taking into account all contributing factors we therefore conclude that conformational changes can indeed contribute to the measured SPR signal.
ER  -

DOBROVODSKÝ, Daniel a Carmelo DI PRIMO. Do conformational changes contribute to the surface plasmon resonance signal? \textit{Biosensors and Bioelectronics}. Elsevier, 2023, roč.~232, July, s.~1-9. ISSN~0956-5663. Dostupné z: https://dx.doi.org/10.1016/j.bios.2023.115296.

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