Hybridization may endanger the rare North Apennine endemic
Cirsium bertolonii

J 2023

Hybridization may endanger the rare North Apennine endemic Cirsium bertolonii

MICHÁLKOVÁ, Ester, Jakub ŠMERDA, Klára PLAČKOVÁ, Aleš KNOLL, Petr BUREŠ et. al.

Basic information

Original name

Hybridization may endanger the rare North Apennine endemic Cirsium bertolonii

Authors

MICHÁLKOVÁ, Ester (203 Czech Republic, belonging to the institution), Jakub ŠMERDA (203 Czech Republic, belonging to the institution), Klára PLAČKOVÁ (203 Czech Republic, belonging to the institution), Aleš KNOLL (203 Czech Republic) and Petr BUREŠ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Plant Systematics and Evolution, Springer, 2023, 0378-2697

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10611 Plant sciences, botany

Country of publisher

Austria

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.900 in 2022

RIV identification code

RIV/00216224:14310/23:00130984

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1007/s00606-023-01854-2

UT WoS

000994303600001

Keywords in English

Asteraceae; Flow cytometry; Genome size; Genomic GC content; Gynodioecy; Homoploid hybridization

Abstract

V originále

We examined populations of North Apennine stenoendemics Cirsium bertolonii in the Apuan Alps and Tuscan-Emilian Apennines and found individuals morphologically shifted to co-occurring C. acaulon or C. erisithales. Hybrid status of these intermediates was confirmed by flow cytometry, morphometrics and amplified fragment length polymorphism (AFLP). We interpreted these hybrids taxonomically as Cirsium xsagrense (C. acaulon x C. bertolonii) and C. xabetonense (C. bertolonii x C. erisithales). Estimated genome size (2C) was 2244 +/- 31 Mbp for C. xsagrense and 2152 +/- 99 Mbp for C. xabetonense. Their genomic GC content was 38.95 +/- 0.35% and 38.77 +/- 0.26%, respectively. Diploid chromosome number 2n = 34 was counted for C. bertolonii, and the previously reported 2n = 12 needs to be considered erroneous. We found C. bertolonii to be a gynodioecious species (like many other Cirsium species) that is not reproductively isolated by ploidy level or chromosome number from co-occurring congeners. The relatively frequent occurrence of C. xsagrense in Monte Sagro (locus classicus of C. bertolonii) and the prevalence of C. xabetonense in Alpe Tre Potenze suggest that hybridization occurs repeatedly in C. bertolonii, as also confirmed by older herbarium specimens. C. xabetonense produces ripe achenes and F2 hybrids or backcrosses, as some other Cirsium hybrids do. Genetic erosion/swamping via interspecific hybridization can therefore pose a risk to the genetic integrity of C. bertolonii, as it does for some other narrowly endemic high-mountain Cirsium species in Europe.

Links

GA20-15989S, research and development project

Name: Evoluce velikosti genomu - centromerický drajv v nové roli (Acronym: Centrogenomtah)

Investor: Czech Science Foundation

MUNI/A/1334/2018, interní kód MU

Name: Dlouhodobé a současné procesy utvářející diverzitu významných evropských biotopů (Acronym: DSP BIOTOP)

Investor: Masaryk University, Category A

Citovat

MICHÁLKOVÁ, Ester, Jakub ŠMERDA, Klára PLAČKOVÁ, Aleš KNOLL and Petr BUREŠ. Hybridization may endanger the rare North Apennine endemic Cirsium bertolonii. Plant Systematics and Evolution. Springer, 2023, vol. 309, No 3, p. 1-21. ISSN 0378-2697. Available from: https://dx.doi.org/10.1007/s00606-023-01854-2.

@article{2291799,
   author = {Michálková, Ester and Šmerda, Jakub and Plačková, Klára and Knoll, Aleš and Bureš, Petr},
   article_number = {3},
   doi = {http://dx.doi.org/10.1007/s00606-023-01854-2},
   keywords = {Asteraceae; Flow cytometry; Genome size; Genomic GC content; Gynodioecy; Homoploid hybridization},
   language = {eng},
   issn = {0378-2697},
   journal = {Plant Systematics and Evolution},
   title = {Hybridization may endanger the rare North Apennine endemic Cirsium bertolonii},
   url = {https://doi.org/10.1007/s00606-023-01854-2},
   volume = {309},
   year = {2023}
}

TY  - JOUR
ID  - 2291799
AU  - Michálková, Ester - Šmerda, Jakub - Plačková, Klára - Knoll, Aleš - Bureš, Petr
PY  - 2023
TI  - Hybridization may endanger the rare North Apennine endemic Cirsium bertolonii
JF  - Plant Systematics and Evolution
VL  - 309
IS  - 3
SP  - 1-21
EP  - 1-21
PB  - Springer
SN  - 03782697
KW  - Asteraceae
KW  - Flow cytometry
KW  - Genome size
KW  - Genomic GC content
KW  - Gynodioecy
KW  - Homoploid hybridization
UR  - https://doi.org/10.1007/s00606-023-01854-2
N2  - We examined populations of North Apennine stenoendemics Cirsium bertolonii in the Apuan Alps and Tuscan-Emilian Apennines and found individuals morphologically shifted to co-occurring C. acaulon or C. erisithales. Hybrid status of these intermediates was confirmed by flow cytometry, morphometrics and amplified fragment length polymorphism (AFLP). We interpreted these hybrids taxonomically as Cirsium xsagrense (C. acaulon x C. bertolonii) and C. xabetonense (C. bertolonii x C. erisithales). Estimated genome size (2C) was 2244 +/- 31 Mbp for C. xsagrense and 2152 +/- 99 Mbp for C. xabetonense. Their genomic GC content was 38.95 +/- 0.35% and 38.77 +/- 0.26%, respectively. Diploid chromosome number 2n = 34 was counted for C. bertolonii, and the previously reported 2n = 12 needs to be considered erroneous. We found C. bertolonii to be a gynodioecious species (like many other Cirsium species) that is not reproductively isolated by ploidy level or chromosome number from co-occurring congeners. The relatively frequent occurrence of C. xsagrense in Monte Sagro (locus classicus of C. bertolonii) and the prevalence of C. xabetonense in Alpe Tre Potenze suggest that hybridization occurs repeatedly in C. bertolonii, as also confirmed by older herbarium specimens. C. xabetonense produces ripe achenes and F2 hybrids or backcrosses, as some other Cirsium hybrids do. Genetic erosion/swamping via interspecific hybridization can therefore pose a risk to the genetic integrity of C. bertolonii, as it does for some other narrowly endemic high-mountain Cirsium species in Europe.
ER  -

MICHÁLKOVÁ, Ester, Jakub ŠMERDA, Klára PLAČKOVÁ, Aleš KNOLL and Petr BUREŠ. Hybridization may endanger the rare North Apennine endemic Cirsium bertolonii. \textit{Plant Systematics and Evolution}. Springer, 2023, vol.~309, No~3, p.~1-21. ISSN~0378-2697. Available from: https://dx.doi.org/10.1007/s00606-023-01854-2.

Detailed Information on Publication Record