Sensitivity enhancement of capillary electrophoresis‐frontal
analysis based method for characterization of drug‐protein
interactions using on‐line sample preconcentration

J 2023

Sensitivity enhancement of capillary electrophoresis‐frontal analysis based method for characterization of drug‐protein interactions using on‐line sample preconcentration

BRŽEZICKÁ, Taťána, Zdeněk GLATZ a Lenka KOHÚTOVÁ

Základní údaje

Originální název

Sensitivity enhancement of capillary electrophoresis‐frontal analysis based method for characterization of drug‐protein interactions using on‐line sample preconcentration

Autoři

BRŽEZICKÁ, Taťána (203 Česká republika, domácí), Zdeněk GLATZ (203 Česká republika, domácí) a Lenka KOHÚTOVÁ (203 Česká republika, garant, domácí)

Vydání

Journal of Separation Science, Weinheim, Wiley, 2023, 1615-9306

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.100 v roce 2022

Kód RIV

RIV/00216224:14310/23:00134152

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1002/jssc.202300152

UT WoS

001017663100001

Klíčová slova anglicky

binding constants; capillary electrophoresis-frontal analysis; human serum albumin; on-line preconcentration

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 26. 1. 2024 10:23, prof. RNDr. Zdeněk Glatz, CSc.

Anotace

V originále

Capillary electrophoresis-frontal analysis is one of the most frequently used approach for the study of plasma protein-drug interactions as a substantial part of the new drug development. However, the capillary electrophoresis-frontal analysis typically combined with ultraviolet visible detection suffers from insufficient concentration sensitivity particularly for substances with limited solubility and low molar absorption coefficient. The sensitivity problem has been solved in this work by its combination with an on-line sample preconcentration. According the knowledge of the authors this combination has never been used to characterize plasma protein-drug binding. It resulted into a fully automated and versatile methodology for the characterization of binding interactions. Further, the validated method minimalizes the experimental errors due to a reduction in the manipulation of samples. Moreover, employing an on-line preconcentration strategy with capillary electrophoresis-frontal analysis using human serum albumin-salicylic acid as a model system improves the drug concentration sensitivity 17 fold compared to the conventional method. The value of binding constant (1.51 ± 0.63)‧104 L/mol obtained by this new capillary electrophoresis-frontal analysis modification is in agreement with the value (1.13 ± 0.28)‧104 L/mol estimated by conventional variant of capillary electrophoresis-frontal analysis without the preconcentration step, as well as with literature data obtained using different techniques.

Návaznosti

GA19-08358S, projekt VaV

Název: Nové přístupy pro studium afinitních interakcí založené na kapilární elektroforéze

Investor: Grantová agentura ČR, Nove přístupy pro studium afinitních interakcí založené na kapilární elektroforéze

MUNI/A/1313/2022, interní kód MU

Název: Podpora biochemického výzkumu v roce 2023

Investor: Masarykova univerzita, Podpora biochemického výzkumu v roce 2023

Citovat

BRŽEZICKÁ, Taťána, Zdeněk GLATZ a Lenka KOHÚTOVÁ. Sensitivity enhancement of capillary electrophoresis‐frontal analysis based method for characterization of drug‐protein interactions using on‐line sample preconcentration. Journal of Separation Science. Weinheim: Wiley, 2023, roč. 46, č. 17, s. 1-8. ISSN 1615-9306. Dostupné z: https://dx.doi.org/10.1002/jssc.202300152.

@article{2293206,
   author = {Bržezická, Taťána and Glatz, Zdeněk and Kohútová, Lenka},
   article_location = {Weinheim},
   article_number = {17},
   doi = {http://dx.doi.org/10.1002/jssc.202300152},
   keywords = {binding constants; capillary electrophoresis-frontal analysis; human serum albumin; on-line preconcentration},
   language = {eng},
   issn = {1615-9306},
   journal = {Journal of Separation Science},
   title = {Sensitivity enhancement of capillary electrophoresis‐frontal analysis based method for characterization of drug‐protein interactions using on‐line sample preconcentration},
   url = {https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/full/10.1002/jssc.202300152?campaign=woletoc},
   volume = {46},
   year = {2023}
}

TY  - JOUR
ID  - 2293206
AU  - Bržezická, Taťána - Glatz, Zdeněk - Kohútová, Lenka
PY  - 2023
TI  - Sensitivity enhancement of capillary electrophoresis‐frontal analysis based method for characterization of drug‐protein interactions using on‐line sample preconcentration
JF  - Journal of Separation Science
VL  - 46
IS  - 17
SP  - 1-8
EP  - 1-8
PB  - Wiley
SN  - 16159306
KW  - binding constants
KW  - capillary electrophoresis-frontal analysis
KW  - human serum albumin
KW  - on-line preconcentration
UR  - https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/full/10.1002/jssc.202300152?campaign=woletoc
N2  - Capillary electrophoresis-frontal analysis is one of the most frequently used approach for the study of plasma protein-drug interactions as a substantial part of the new drug development. However, the capillary electrophoresis-frontal analysis typically combined with ultraviolet visible detection suffers from insufficient concentration sensitivity particularly for substances with limited solubility and low molar absorption coefficient. The sensitivity problem has been solved in this work by its combination with an on-line sample preconcentration. According the knowledge of the authors this combination has never been used to characterize plasma protein-drug binding. It resulted into a fully automated and versatile methodology for the characterization of binding interactions. Further, the validated method minimalizes the experimental errors due to a reduction in the manipulation of samples. Moreover, employing an on-line preconcentration strategy with capillary electrophoresis-frontal analysis using human serum albumin-salicylic acid as a model system improves the drug concentration sensitivity 17 fold compared to the conventional method. The value of binding constant (1.51 ± 0.63)‧104 L/mol obtained by this new capillary electrophoresis-frontal analysis modification is in agreement with the value (1.13 ± 0.28)‧104 L/mol estimated by conventional variant of capillary electrophoresis-frontal analysis without the preconcentration step, as well as with literature data obtained using different techniques.
ER  -

BRŽEZICKÁ, Taťána, Zdeněk GLATZ a Lenka KOHÚTOVÁ. Sensitivity enhancement of capillary electrophoresis‐frontal analysis based method for characterization of drug‐protein interactions using on‐line sample preconcentration. \textit{Journal of Separation Science}. Weinheim: Wiley, 2023, roč.~46, č.~17, s.~1-8. ISSN~1615-9306. Dostupné z: https://dx.doi.org/10.1002/jssc.202300152.

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