Hormone-regulated expansins: Expression, localization, and cell
wall biomechanics in Arabidopsis root growth

J 2024

Hormone-regulated expansins: Expression, localization, and cell wall biomechanics in Arabidopsis root growth

ŠÁMALOVÁ, Markéta; Alesia MELNIKAVA; Kareem ELSAYAD; Alexis PEAUCELLE; Evelína GAHUROVÁ et. al.

Základní údaje

Originální název

Hormone-regulated expansins: Expression, localization, and cell wall biomechanics in Arabidopsis root growth

Autoři

ŠÁMALOVÁ, Markéta (203 Česká republika, domácí); Alesia MELNIKAVA (112 Bělorusko, domácí); Kareem ELSAYAD; Alexis PEAUCELLE; Evelína GAHUROVÁ (203 Česká republika, domácí); Jaromír GUMULEC (203 Česká republika, domácí); Ioannis SPYROGLOU (300 Řecko, domácí); Elena V ZEMLYANSKAYA; Elena V UBOGOEVA; Darina BALKOVÁ (703 Slovensko, domácí); Martin DEMKO (703 Slovensko, domácí); Nicolas BLAVET (250 Francie, domácí); Panagiotis ALEXIOU (300 Řecko, domácí); Vladimir BENES (276 Německo); Gregory MOUILLE (250 Francie) a Jan HEJÁTKO (203 Česká republika, garant, domácí)

Vydání

Plant Physiology, CARY, OXFORD UNIV PRESS INC, 2024, 0032-0889

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.600 v roce 2023

Kód RIV

RIV/00216224:14740/24:00135141

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1093/plphys/kiad228

UT WoS

000980114700001

EID Scopus

2-s2.0-85181561895

Klíčová slova anglicky

GENE-EXPRESSION; ORGAN INITIATION; ALPHA-EXPANSIN; CYTOKININ; STRESS; AUXIN

Štítky

14110518, CF BIOIT, CF CELLIM, CF PLANT, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 9. 10. 2024 13:25, Mgr. Adéla Pešková

Anotace

V originále

Expansins facilitate cell expansion by mediating pH-dependent cell wall (CW) loosening. However, the role of expansins in controlling CW biomechanical properties in specific tissues and organs remains elusive. We monitored hormonal responsiveness and spatial specificity of expression and localization of expansins predicted to be the direct targets of cytokinin signaling in Arabidopsis (Arabidopsis thaliana). We found EXPANSIN1 (EXPA1) homogenously distributed throughout the CW of columella/lateral root cap, while EXPA10 and EXPA14 localized predominantly at 3-cell boundaries in the epidermis/cortex in various root zones. EXPA15 revealed cell-type-specific combination of homogenous vs. 3-cell boundaries localization. By comparing Brillouin frequency shift and AFM-measured Young's modulus, we demonstrated Brillouin light scattering (BLS) as a tool suitable for non-invasive in vivo quantitative assessment of CW viscoelasticity. Using both BLS and AFM, we showed that EXPA1 overexpression upregulated CW stiffness in the root transition zone (TZ). The dexamethasone-controlled EXPA1 overexpression induced fast changes in the transcription of numerous CW-associated genes, including several EXPAs and XYLOGLUCAN:XYLOGLUCOSYL TRANSFERASEs (XTHs), and associated with rapid pectin methylesterification determined by in situ Fourier-transform infrared spectroscopy in the root TZ. The EXPA1-induced CW remodeling is associated with the shortening of the root apical meristem, leading to root growth arrest. Based on our results, we propose that expansins control root growth by a delicate orchestration of CW biomechanical properties, possibly regulating both CW loosening and CW remodeling. Expansins control root growth by orchestrating cell wall biomechanical properties, possibly regulating cell wall stiffening and root growth arrest associated with cell wall remodeling.

Návaznosti

EF16_019/0000738, projekt VaV

Název: Centrum experimentální biologie rostlin

GA19-24753S, projekt VaV

Název: Struktura a funkce dirigent proteinů řízených hormony u Arabidopsis thaliana

Investor: Grantová agentura ČR, Struktura a funkce dirigent proteinů řízených hormony u Arabidopsis thaliana

GA22-17501S, projekt VaV

Název: Role biomechaniky buněčné stěny v regulaci růstu a vývoje kořene Arabidopsis

Investor: Grantová agentura ČR, Role biomechaniky buněčné stěny v regulaci růstu a vývoje kořene Arabidopsis

LTAUSA18161, projekt VaV

Název: Význam hormonálních interakcí v kontrole vlastností buněčné stěny Arabidopsis thaliana

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Význam hormonálních interakcí v kontrole vlastností buněčné stěny Arabidopsis thaliana, INTER-ACTION

90129, velká výzkumná infrastruktura

Název: Czech-BioImaging II

90267, velká výzkumná infrastruktura

Název: NCMG III

Přiložené soubory

Hormone-regulated_expansins_Expression__localization__and_cell_wall_biomechanics_in_Arabidopsis_root_growth.pdf

Citovat

ŠÁMALOVÁ, Markéta; Alesia MELNIKAVA; Kareem ELSAYAD; Alexis PEAUCELLE; Evelína GAHUROVÁ; Jaromír GUMULEC; Ioannis SPYROGLOU; Elena V ZEMLYANSKAYA; Elena V UBOGOEVA; Darina BALKOVÁ; Martin DEMKO; Nicolas BLAVET; Panagiotis ALEXIOU; Vladimir BENES; Gregory MOUILLE a Jan HEJÁTKO. Hormone-regulated expansins: Expression, localization, and cell wall biomechanics in Arabidopsis root growth. Plant Physiology. CARY: OXFORD UNIV PRESS INC, 2024, roč. 194, č. 1, s. 209-228. ISSN 0032-0889. Dostupné z: https://dx.doi.org/10.1093/plphys/kiad228.

@article{2294400,
   author = {Šámalová, Markéta and Melnikava, Alesia and Elsayad, Kareem and Peaucelle, Alexis and Gahurová, Evelína and Gumulec, Jaromír and Spyroglou, Ioannis and Zemlyanskaya, Elena V and Ubogoeva, Elena V and Balková, Darina and Demko, Martin and Blavet, Nicolas and Alexiou, Panagiotis and Benes, Vladimir and Mouille, Gregory and Hejátko, Jan},
   article_location = {CARY},
   article_number = {1},
   doi = {http://dx.doi.org/10.1093/plphys/kiad228},
   keywords = {GENE-EXPRESSION; ORGAN INITIATION; ALPHA-EXPANSIN; CYTOKININ; STRESS; AUXIN},
   language = {eng},
   issn = {0032-0889},
   journal = {Plant Physiology},
   title = {Hormone-regulated expansins: Expression, localization, and cell wall biomechanics in Arabidopsis root growth},
   url = {https://academic.oup.com/plphys/advance-article/doi/10.1093/plphys/kiad228/7129196?login=true},
   volume = {194},
   year = {2024}
}

TY  - JOUR
ID  - 2294400
AU  - Šámalová, Markéta - Melnikava, Alesia - Elsayad, Kareem - Peaucelle, Alexis - Gahurová, Evelína - Gumulec, Jaromír - Spyroglou, Ioannis - Zemlyanskaya, Elena V - Ubogoeva, Elena V - Balková, Darina - Demko, Martin - Blavet, Nicolas - Alexiou, Panagiotis - Benes, Vladimir - Mouille, Gregory - Hejátko, Jan
PY  - 2024
TI  - Hormone-regulated expansins: Expression, localization, and cell wall biomechanics in Arabidopsis root growth
JF  - Plant Physiology
VL  - 194
IS  - 1
SP  - 209-228
EP  - 209-228
PB  - OXFORD UNIV PRESS INC
SN  - 00320889
KW  - GENE-EXPRESSION
KW  - ORGAN INITIATION
KW  - ALPHA-EXPANSIN
KW  - CYTOKININ
KW  - STRESS
KW  - AUXIN
UR  - https://academic.oup.com/plphys/advance-article/doi/10.1093/plphys/kiad228/7129196?login=true
N2  - Expansins facilitate cell expansion by mediating pH-dependent cell wall (CW) loosening. However, the role of expansins in controlling CW biomechanical properties in specific tissues and organs remains elusive. We monitored hormonal responsiveness and spatial specificity of expression and localization of expansins predicted to be the direct targets of cytokinin signaling in Arabidopsis (Arabidopsis thaliana). We found EXPANSIN1 (EXPA1) homogenously distributed throughout the CW of columella/lateral root cap, while EXPA10 and EXPA14 localized predominantly at 3-cell boundaries in the epidermis/cortex in various root zones. EXPA15 revealed cell-type-specific combination of homogenous vs. 3-cell boundaries localization. By comparing Brillouin frequency shift and AFM-measured Young's modulus, we demonstrated Brillouin light scattering (BLS) as a tool suitable for non-invasive in vivo quantitative assessment of CW viscoelasticity. Using both BLS and AFM, we showed that EXPA1 overexpression upregulated CW stiffness in the root transition zone (TZ). The dexamethasone-controlled EXPA1 overexpression induced fast changes in the transcription of numerous CW-associated genes, including several EXPAs and XYLOGLUCAN:XYLOGLUCOSYL TRANSFERASEs (XTHs), and associated with rapid pectin methylesterification determined by in situ Fourier-transform infrared spectroscopy in the root TZ. The EXPA1-induced CW remodeling is associated with the shortening of the root apical meristem, leading to root growth arrest. Based on our results, we propose that expansins control root growth by a delicate orchestration of CW biomechanical properties, possibly regulating both CW loosening and CW remodeling. Expansins control root growth by orchestrating cell wall biomechanical properties, possibly regulating cell wall stiffening and root growth arrest associated with cell wall remodeling.
ER  -

ŠÁMALOVÁ, Markéta; Alesia MELNIKAVA; Kareem ELSAYAD; Alexis PEAUCELLE; Evelína GAHUROVÁ; Jaromír GUMULEC; Ioannis SPYROGLOU; Elena V ZEMLYANSKAYA; Elena V UBOGOEVA; Darina BALKOVÁ; Martin DEMKO; Nicolas BLAVET; Panagiotis ALEXIOU; Vladimir BENES; Gregory MOUILLE a Jan HEJÁTKO. Hormone-regulated expansins: Expression, localization, and cell wall biomechanics in Arabidopsis root growth. \textit{Plant Physiology}. CARY: OXFORD UNIV PRESS INC, 2024, roč.~194, č.~1, s.~209-228. ISSN~0032-0889. Dostupné z: https://dx.doi.org/10.1093/plphys/kiad228.

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