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@article{2295018,
author = {Peterková, Kristýna and Vorel, Jiří and Ilgová, Jana and Ostašov, Pavel and Fajtová, Pavla and Konečný, Lukáš and Chánova, Marta and Kašný, Martin and Horn, Martin and Dvořák, Jan},
article_location = {Oxford},
article_number = {5-6},
doi = {http://dx.doi.org/10.1016/j.ijpara.2022.12.007},
keywords = {Schistosoma mansoni; Egg; Fasciola hepatica; Comparative transcriptomics; Protease; Protease inhibitor},
language = {eng},
issn = {0020-7519},
journal = {International Journal for Parasitology},
title = {Proteases and their inhibitors involved in Schistosoma mansoni egg-host interaction revealed by comparative transcriptomics with Fasciola hepatica eggs},
url = {https://doi.org/10.1016/j.ijpara.2022.12.007},
volume = {53},
year = {2023}
}
TY - JOUR
ID - 2295018
AU - Peterková, Kristýna - Vorel, Jiří - Ilgová, Jana - Ostašov, Pavel - Fajtová, Pavla - Konečný, Lukáš - Chánova, Marta - Kašný, Martin - Horn, Martin - Dvořák, Jan
PY - 2023
TI - Proteases and their inhibitors involved in Schistosoma mansoni egg-host interaction revealed by comparative transcriptomics with Fasciola hepatica eggs
JF - International Journal for Parasitology
VL - 53
IS - 5-6
SP - 253-263
EP - 253-263
PB - Elsevier Science Ltd.
SN - 00207519
KW - Schistosoma mansoni
KW - Egg
KW - Fasciola hepatica
KW - Comparative transcriptomics
KW - Protease
KW - Protease inhibitor
UR - https://doi.org/10.1016/j.ijpara.2022.12.007
N2 - Schistosoma mansoni eggs are the main causative agents of the pathological manifestations of schistoso-miasis. The eggs are laid in the host bloodstream, then they migrate through the intestinal wall into the lumen. However, a significant proportion of the eggs become lodged in the liver, where they cause inflammation and fibrosis. In this study, we focus on a specific group of proteins expressed by the egg, namely proteases and their inhibitors. These molecules are often involved in schistosome-host interac-tions, but are still unexplored in the egg stage. Using RNA-seq and comparative transcriptomics of imma-ture and mature S. mansoni eggs, we mapped the portfolio of proteases and their inhibitors, and determined their gene expression levels. In addition, we compared these data with gene expression of proteases and their inhibitors in Fasciola hepatica eggs. Fasciola hepatica eggs served as a useful compar-ative model, as they do not migrate through tissues and inflict pathology. We detected transcription of 135 and 117 proteases in S. mansoni and F. hepatica eggs, respectively, with 87 identified as orthologous between the two species. In contrast, we observed only four orthologous inhibitors out of 21 and 16 iden-tified in S. mansoni and F. hepatica eggs, respectively. Among others, we measured high and developmen-tally regulated levels of expression of metalloproteases in S. mansoni eggs, specifically aminopeptidase N1, endothelin-converting enzyme 1, and several leishmanolysin-like peptidases. We identified highly transcribed protease inhibitors serpin and alpha-2-macroglobulin that are unique to S. mansoni eggs, and antistasin-like inhibitor in F. hepatica eggs. This study provides new insights into the portfolio of pro -teases and inhibitors expressed by S. mansoni with potential roles in egg tissue migration, stimulation of angiogenesis, and interaction with host blood and immunity.
ER -
PETERKOVÁ, Kristýna, Jiří VOREL, Jana ILGOVÁ, Pavel OSTAŠOV, Pavla FAJTOVÁ, Lukáš KONEČNÝ, Marta CHÁNOVA, Martin KAŠNÝ, Martin HORN a Jan DVOŘÁK. Proteases and their inhibitors involved in Schistosoma mansoni egg-host interaction revealed by comparative transcriptomics with Fasciola hepatica eggs. \textit{International Journal for Parasitology}. Oxford: Elsevier Science Ltd., 2023, roč.~53, 5-6, s.~253-263. ISSN~0020-7519. Dostupné z: https://dx.doi.org/10.1016/j.ijpara.2022.12.007.
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