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@article{2296942,
author = {Brzuska, Gabriela and Zimna, Marta and Baranska, Klaudia and Szewczyk, Boguslaw and Strakova, Petra and Růžek, Daniel and Krol, Ewelina},
article_number = {3},
doi = {http://dx.doi.org/10.1128/spectrum.02564-22},
keywords = {SARS-CoV-2; adjuvants; antigen specificity; vaccines},
language = {eng},
issn = {2165-0497},
journal = {Microbiology Spectrum},
title = {The Influence of Adjuvant Type on the Immunogenicity of RBD/N Cocktail Antigens as a Vaccine Candidate against SARS-CoV-2 Virus.},
url = {https://doi.org/10.1128/spectrum.02564-22},
volume = {11},
year = {2023}
}
TY - JOUR
ID - 2296942
AU - Brzuska, Gabriela - Zimna, Marta - Baranska, Klaudia - Szewczyk, Boguslaw - Strakova, Petra - Růžek, Daniel - Krol, Ewelina
PY - 2023
TI - The Influence of Adjuvant Type on the Immunogenicity of RBD/N Cocktail Antigens as a Vaccine Candidate against SARS-CoV-2 Virus.
JF - Microbiology Spectrum
VL - 11
IS - 3
SP - 1-14
EP - 1-14
PB - American Society for Microbiology
SN - 21650497
KW - SARS-CoV-2
KW - adjuvants
KW - antigen specificity
KW - vaccines
UR - https://doi.org/10.1128/spectrum.02564-22
N2 - The emerging virus SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2 virus), agent of COVID-19, appeared in December 2019 in Wuhan, China, and became a serious threat to global health and public safety. Many COVID-19 vaccines have been approved and licensed around the world. Most of the developed vaccines include S protein and induce an antibody-based immune response. Additionally, T-cell response to the SARS-CoV-2 antigens could be beneficial for combating the infection. The type of immune response is greatly dependent not only on the antigen, but also on adjuvants used in vaccine formulation. Here, we compared the effect of four different adjuvants (AddaS03, Alhydrogel/MPLA, Alhydrogel/ODN2395, Quil A) on the immunogenicity of a mixture of recombinant RBD and N SARS-CoV-2 proteins. We have analyzed the antibody and T-cell response specific to RBD and N proteins and assessed the impact of adjuvants on virus neutralization. Our results clearly indicated that Alhydrogel/MPLA and Alhydrogel/ODN2395 adjuvants elicited the higher titers of specific and cross-reactive antibodies to S protein variants from various SARS-CoV-2 and SARS-CoV-1 strains. Moreover, Alhydrogel/ODN2395 stimulated high cellular response to both antigens, as assessed by IFN-γ production. Importantly, sera collected from mice immunized with RBD/N cocktail in combination with these adjuvants exhibited neutralizing activity against the authentic SARS-CoV-2 virus as well as particles pseudotyped with S protein from various virus variants. The results from our study demonstrate the immunogenic potential of RBD and N antigens and point out the importance of adjuvants selection in vaccine formulation in order to enhance the immunological response.
ER -
BRZUSKA, Gabriela, Marta ZIMNA, Klaudia BARANSKA, Boguslaw SZEWCZYK, Petra STRAKOVA, Daniel RŮŽEK a Ewelina KROL. The Influence of Adjuvant Type on the Immunogenicity of RBD/N Cocktail Antigens as a Vaccine Candidate against SARS-CoV-2 Virus. \textit{Microbiology Spectrum}. American Society for Microbiology, 2023, roč.~11, č.~3, s.~1-14. ISSN~2165-0497. Dostupné z: https://dx.doi.org/10.1128/spectrum.02564-22.
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