Insight into peculiar adhesion of cells to plasma-chemically
prepared multifunctional “amino-glue” surfaces

J 2023

Insight into peculiar adhesion of cells to plasma-chemically prepared multifunctional “amino-glue” surfaces

BUCHTELOVÁ, Martina, Lucie BLAHOVÁ, David NEČAS, Petra KŘÍŽKOVÁ, Jana BARTOŠÍKOVÁ et. al.

Základní údaje

Originální název

Insight into peculiar adhesion of cells to plasma-chemically prepared multifunctional “amino-glue” surfaces

Autoři

BUCHTELOVÁ, Martina, Lucie BLAHOVÁ, David NEČAS, Petra KŘÍŽKOVÁ (203 Česká republika, domácí), Jana BARTOŠÍKOVÁ (203 Česká republika, domácí), Jiřina MEDALOVÁ (203 Česká republika, domácí), Zdeňka KOLSKÁ, Dirk HEGEMANN a Lenka ZAJÍČKOVÁ (203 Česká republika, garant, domácí)

Vydání

Plasma Processes and Polymers, Wiley‐VCH GmbH, 2023, 1612-8850

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

20506 Coating and films

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.500 v roce 2022

Kód RIV

RIV/00216224:14310/23:00131232

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1002/ppap.202200157

UT WoS

000941082700001

Klíčová slova anglicky

amine plasma polymer; carboxyl plasma polymer; cell adhesion; glycocalyx; nonspecificadhesion

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 8. 4. 2024 07:48, Mgr. Marie Šípková, DiS.

Anotace

V originále

Plasma polymers (PPs) can easily modify material surfaces to improve their bio-applicability due to match-made surface-free energy and functionality. However, cell adhesion to PPs typically composed of various functional groups has not yet been fully understood. We explain the origin of strong resistance to trypsin treatment previously noted for nonendothelial cells on amine PPs. It is caused mainly by nonspecific adhesion of negatively charged parts of transmembrane proteins to the positively charged amine PP surface, enabled by thin glycocalyx. However, endothelial cells are bound primarily by their thick, negatively charged glycocalyx and sporadically by integrins in kinetic traps, both cleaved by trypsin. Cell scratching by atomic force microscopy tip confirmed the correlation of trypsin resistance to the strength of cell adhesion.

Návaznosti

LM2018127, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

90110, velká výzkumná infrastruktura

Název: CzechNanoLab

90242, velká výzkumná infrastruktura

Název: CIISB III

Citovat

BUCHTELOVÁ, Martina, Lucie BLAHOVÁ, David NEČAS, Petra KŘÍŽKOVÁ, Jana BARTOŠÍKOVÁ, Jiřina MEDALOVÁ, Zdeňka KOLSKÁ, Dirk HEGEMANN a Lenka ZAJÍČKOVÁ. Insight into peculiar adhesion of cells to plasma-chemically prepared multifunctional “amino-glue” surfaces. Plasma Processes and Polymers. Wiley‐VCH GmbH, 2023, roč. 20, č. 6, s. 1-15. ISSN 1612-8850. Dostupné z: https://dx.doi.org/10.1002/ppap.202200157.

@article{2297299,
   author = {Buchtelová, Martina and Blahová, Lucie and Nečas, David and Křížková, Petra and Bartošíková, Jana and Medalová, Jiřina and Kolská, Zdeňka and Hegemann, Dirk and Zajíčková, Lenka},
   article_number = {6},
   doi = {http://dx.doi.org/10.1002/ppap.202200157},
   keywords = {amine plasma polymer; carboxyl plasma polymer; cell adhesion; glycocalyx; nonspecificadhesion},
   language = {eng},
   issn = {1612-8850},
   journal = {Plasma Processes and Polymers},
   title = {Insight into peculiar adhesion of cells to plasma-chemically prepared multifunctional “amino-glue” surfaces},
   url = {https://onlinelibrary.wiley.com/doi/10.1002/ppap.202200157},
   volume = {20},
   year = {2023}
}

TY  - JOUR
ID  - 2297299
AU  - Buchtelová, Martina - Blahová, Lucie - Nečas, David - Křížková, Petra - Bartošíková, Jana - Medalová, Jiřina - Kolská, Zdeňka - Hegemann, Dirk - Zajíčková, Lenka
PY  - 2023
TI  - Insight into peculiar adhesion of cells to plasma-chemically prepared multifunctional “amino-glue” surfaces
JF  - Plasma Processes and Polymers
VL  - 20
IS  - 6
SP  - 1-15
EP  - 1-15
PB  - Wiley‐VCH GmbH
SN  - 16128850
KW  - amine plasma polymer
KW  - carboxyl plasma polymer
KW  - cell adhesion
KW  - glycocalyx
KW  - nonspecificadhesion
UR  - https://onlinelibrary.wiley.com/doi/10.1002/ppap.202200157
N2  - Plasma polymers (PPs) can easily modify material surfaces to improve their bio-applicability due to match-made surface-free energy and functionality. However, cell adhesion to PPs typically composed of various functional groups has not yet been fully understood. We explain the origin of strong resistance to trypsin treatment previously noted for nonendothelial cells on amine PPs. It is caused mainly by nonspecific adhesion of negatively charged parts of transmembrane proteins to the positively charged amine PP surface, enabled by thin glycocalyx. However, endothelial cells are bound primarily by their thick, negatively charged glycocalyx and sporadically by integrins in kinetic traps, both cleaved by trypsin. Cell scratching by atomic force microscopy tip confirmed the correlation of trypsin resistance to the strength of cell adhesion.
ER  -

BUCHTELOVÁ, Martina, Lucie BLAHOVÁ, David NEČAS, Petra KŘÍŽKOVÁ, Jana BARTOŠÍKOVÁ, Jiřina MEDALOVÁ, Zdeňka KOLSKÁ, Dirk HEGEMANN a Lenka ZAJÍČKOVÁ. Insight into peculiar adhesion of cells to plasma-chemically prepared multifunctional “amino-glue” surfaces. \textit{Plasma Processes and Polymers}. Wiley‐VCH GmbH, 2023, roč.~20, č.~6, s.~1-15. ISSN~1612-8850. Dostupné z: https://dx.doi.org/10.1002/ppap.202200157.

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