BEHERA, Susrisweta, Lukáš VESELÝ, Radim ŠTŮSEK, Astrid HAUPTMANN, Thomas LOERTING a Dominik HEGER. Investigating freezing-induced acidity changes in citrate buffers. International Journal of Pharmaceutics. Elsevier B.V., 2023, roč. 643, August, s. 1-12. ISSN 0378-5173. Dostupné z: https://dx.doi.org/10.1016/j.ijpharm.2023.123211. |
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@article{2297377, author = {Behera, Susrisweta and Veselý, Lukáš and Štůsek, Radim and Hauptmann, Astrid and Loerting, Thomas and Heger, Dominik}, article_number = {August}, doi = {http://dx.doi.org/10.1016/j.ijpharm.2023.123211}, keywords = {Stabilization; Freeze-concentrated solution; Hammett acidity function; Differential Scanning Calorimetry; Glass Transition Temperature; Cryomicroscopy; Sulfonephthalein indicators}, language = {eng}, issn = {0378-5173}, journal = {International Journal of Pharmaceutics}, title = {Investigating freezing-induced acidity changes in citrate buffers}, url = {https://doi.org/10.1016/j.ijpharm.2023.123211}, volume = {643}, year = {2023} }
TY - JOUR ID - 2297377 AU - Behera, Susrisweta - Veselý, Lukáš - Štůsek, Radim - Hauptmann, Astrid - Loerting, Thomas - Heger, Dominik PY - 2023 TI - Investigating freezing-induced acidity changes in citrate buffers JF - International Journal of Pharmaceutics VL - 643 IS - August SP - 1-12 EP - 1-12 PB - Elsevier B.V. SN - 03785173 KW - Stabilization KW - Freeze-concentrated solution KW - Hammett acidity function KW - Differential Scanning Calorimetry KW - Glass Transition Temperature KW - Cryomicroscopy KW - Sulfonephthalein indicators UR - https://doi.org/10.1016/j.ijpharm.2023.123211 N2 - Citrate buffers are commonly utilized in the field of biomolecule stabilization. We investigate their applicability in the frozen state within a range of initial pHs (2.5 to 8.0) and concentrations (0.02 to 0.60 M). Citrate buffer solutions subjected to various cooling and heating temperatures are examined in terms of the freezing-induced acidity changes, revealing that citrate buffers acidify upon cooling. The acidity is assessed with sulfonephthalein molecular probes frozen in the samples. Optical cryomicroscopy combined with differential scanning calorimetry was employed to investigate the causes of the observed acidity changes. The buffers partly crystallize and partly vitrify in the ice matrix; these processes influence the resulting pH and allow designing the optimal storage temperatures in the frozen state. The freezing-induced acidification apparently depends on the buffer concentration; at each pH, we suggest pertinent concentration, at which freezing causes minimal acidification. ER -
BEHERA, Susrisweta, Lukáš VESELÝ, Radim ŠTŮSEK, Astrid HAUPTMANN, Thomas LOERTING a Dominik HEGER. Investigating freezing-induced acidity changes in citrate buffers. \textit{International Journal of Pharmaceutics}. Elsevier B.V., 2023, roč.~643, August, s.~1-12. ISSN~0378-5173. Dostupné z: https://dx.doi.org/10.1016/j.ijpharm.2023.123211.
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