Investigating freezing-induced acidity changes in citrate
buffers

J 2023

Investigating freezing-induced acidity changes in citrate buffers

BEHERA, Susrisweta, Lukáš VESELÝ, Radim ŠTŮSEK, Astrid HAUPTMANN, Thomas LOERTING et. al.

Základní údaje

Originální název

Investigating freezing-induced acidity changes in citrate buffers

Autoři

BEHERA, Susrisweta (356 Indie, domácí), Lukáš VESELÝ (203 Česká republika, domácí), Radim ŠTŮSEK (203 Česká republika, domácí), Astrid HAUPTMANN, Thomas LOERTING a Dominik HEGER (203 Česká republika, garant, domácí)

Vydání

International Journal of Pharmaceutics, Elsevier B.V. 2023, 0378-5173

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10400 1.4 Chemical sciences

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.800 v roce 2022

Kód RIV

RIV/00216224:14310/23:00131239

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.ijpharm.2023.123211

UT WoS

001049188600001

Klíčová slova anglicky

Stabilization; Freeze-concentrated solution; Hammett acidity function; Differential Scanning Calorimetry; Glass Transition Temperature; Cryomicroscopy; Sulfonephthalein indicators

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 30. 1. 2024 20:43, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Citrate buffers are commonly utilized in the field of biomolecule stabilization. We investigate their applicability in the frozen state within a range of initial pHs (2.5 to 8.0) and concentrations (0.02 to 0.60 M). Citrate buffer solutions subjected to various cooling and heating temperatures are examined in terms of the freezing-induced acidity changes, revealing that citrate buffers acidify upon cooling. The acidity is assessed with sulfonephthalein molecular probes frozen in the samples. Optical cryomicroscopy combined with differential scanning calorimetry was employed to investigate the causes of the observed acidity changes. The buffers partly crystallize and partly vitrify in the ice matrix; these processes influence the resulting pH and allow designing the optimal storage temperatures in the frozen state. The freezing-induced acidification apparently depends on the buffer concentration; at each pH, we suggest pertinent concentration, at which freezing causes minimal acidification.

Návaznosti

CIII-SI-1312-04-2122-M-158101, interní kód MU

Název: CEEPUS síť "Water - a common but anomalous substance that has to be taught and studied"

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEEPUS síť "Water - a common but anomalous substance that has to be taught and studied"

EF19_073/0016943, projekt VaV

Název: Interní grantová agentura Masarykovy univerzity

MUNI/A/1421/2022, interní kód MU

Název: Vývoj metod a instrumentace pro analýzu biologicky významných látek 2023

Investor: Masarykova univerzita, Vývoj metod a instrumentace pro analýzu biologicky významných látek 2023

MUNI/IGA/0991/2021, interní kód MU

Název: Minimizing freezing-induced acidity changes to ensure the stabilization of biochemicals

Investor: Masarykova univerzita, Minimizing freezing-induced acidity changes to ensure the stabilization of biochemicals

Citovat

BEHERA, Susrisweta, Lukáš VESELÝ, Radim ŠTŮSEK, Astrid HAUPTMANN, Thomas LOERTING a Dominik HEGER. Investigating freezing-induced acidity changes in citrate buffers. International Journal of Pharmaceutics. Elsevier B.V., 2023, roč. 643, August, s. 1-12. ISSN 0378-5173. Dostupné z: https://dx.doi.org/10.1016/j.ijpharm.2023.123211.

@article{2297377,
   author = {Behera, Susrisweta and Veselý, Lukáš and Štůsek, Radim and Hauptmann, Astrid and Loerting, Thomas and Heger, Dominik},
   article_number = {August},
   doi = {http://dx.doi.org/10.1016/j.ijpharm.2023.123211},
   keywords = {Stabilization; Freeze-concentrated solution; Hammett acidity function; Differential Scanning Calorimetry; Glass Transition Temperature; Cryomicroscopy; Sulfonephthalein indicators},
   language = {eng},
   issn = {0378-5173},
   journal = {International Journal of Pharmaceutics},
   title = {Investigating freezing-induced acidity changes in citrate buffers},
   url = {https://doi.org/10.1016/j.ijpharm.2023.123211},
   volume = {643},
   year = {2023}
}

TY  - JOUR
ID  - 2297377
AU  - Behera, Susrisweta - Veselý, Lukáš - Štůsek, Radim - Hauptmann, Astrid - Loerting, Thomas - Heger, Dominik
PY  - 2023
TI  - Investigating freezing-induced acidity changes in citrate buffers
JF  - International Journal of Pharmaceutics
VL  - 643
IS  - August
SP  - 1-12
EP  - 1-12
PB  - Elsevier B.V.
SN  - 03785173
KW  - Stabilization
KW  - Freeze-concentrated solution
KW  - Hammett acidity function
KW  - Differential Scanning Calorimetry
KW  - Glass Transition Temperature
KW  - Cryomicroscopy
KW  - Sulfonephthalein indicators
UR  - https://doi.org/10.1016/j.ijpharm.2023.123211
N2  - Citrate buffers are commonly utilized in the field of biomolecule stabilization. We investigate their applicability in the frozen state within a range of initial pHs (2.5 to 8.0) and concentrations (0.02 to 0.60 M). Citrate buffer solutions subjected to various cooling and heating temperatures are examined in terms of the freezing-induced acidity changes, revealing that citrate buffers acidify upon cooling. The acidity is assessed with sulfonephthalein molecular probes frozen in the samples. Optical cryomicroscopy combined with differential scanning calorimetry was employed to investigate the causes of the observed acidity changes. The buffers partly crystallize and partly vitrify in the ice matrix; these processes influence the resulting pH and allow designing the optimal storage temperatures in the frozen state. The freezing-induced acidification apparently depends on the buffer concentration; at each pH, we suggest pertinent concentration, at which freezing causes minimal acidification.
ER  -

BEHERA, Susrisweta, Lukáš VESELÝ, Radim ŠTŮSEK, Astrid HAUPTMANN, Thomas LOERTING a Dominik HEGER. Investigating freezing-induced acidity changes in citrate buffers. \textit{International Journal of Pharmaceutics}. Elsevier B.V., 2023, roč.~643, August, s.~1-12. ISSN~0378-5173. Dostupné z: https://dx.doi.org/10.1016/j.ijpharm.2023.123211.

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