Detailed Information on Publication Record
2023
Advanced Microscopic and Biosensing Approaches for Monitoring Bacteriophage-Mediated Lysis of Bacteria
ŠIMEČKOVÁ, Hana, Radka OBOŘILOVÁ, Matěj PASTUCHA, Jan PŘIBYL, Petr SKLÁDAL et. al.Basic information
Original name
Advanced Microscopic and Biosensing Approaches for Monitoring Bacteriophage-Mediated Lysis of Bacteria
Authors
ŠIMEČKOVÁ, Hana (203 Czech Republic, belonging to the institution), Radka OBOŘILOVÁ (203 Czech Republic, belonging to the institution), Matěj PASTUCHA (203 Czech Republic, belonging to the institution), Jan PŘIBYL (203 Czech Republic, belonging to the institution), Petr SKLÁDAL (203 Czech Republic, belonging to the institution), Roman PANTŮČEK (203 Czech Republic, belonging to the institution), Zdeněk FARKA (203 Czech Republic, belonging to the institution) and Ivana MAŠLAŇOVÁ (203 Czech Republic, guarantor, belonging to the institution)
Edition
6th World Congress on Targeting Phage Therapy, 2023
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
10608 Biochemistry and molecular biology
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
RIV identification code
RIV/00216224:14310/23:00131316
Organization unit
Faculty of Science
Keywords in English
Bacteriophages; Phage Therapy; Atomic Force Microscopy; Surface Plasmon Resonance; Biosensing; Lysis
Tags
International impact
Změněno: 18/12/2023 15:38, prof. RNDr. Roman Pantůček, Ph.D.
Abstract
V originále
Phage therapy uses lytic phages to kill the bacterial host as a result of the virus life cycle, thus working with dynamic, living, and evolving entities. For the development and optimization of effective phage-based treatments, it is essential to have a thorough understanding of the mechanisms that participate in the process of phage-mediated bacterial lysis. Promising new approaches such as atomic force microscopy (AFM) and surface plasmon resonance (SPR) biosensing can be utilized for this purpose. Both are sensitive and rapid analytical methods that provide detailed insight into how phages interact with bacterial cells and allow for monitoring immobilized cells under native conditions in real-time. In our study, we investigated the efficacy of lytic antimicrobials against the pathogenic bacterium Staphylococcus aureus. Specifically, we explored the mechanisms of bacterial lysis mediated by the phage vB_SauP_P68 (P68) and the enzyme lysostaphin. AFM enabled a high-resolution investigation of topographical and biomechanical properties at the single-cell level. The SPR experiments completed the work with the information on the kinetics of agent-bacterium interaction. The results are expected to advance the development of this promising field, combat bacterial infections and support the exploitation of phage therapy.
Links
| LM2023042, research and development project |
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| MUNI/A/1325/2021, interní kód MU |
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| NU22-05-00042, research and development project |
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