SMC5/6 complex-mediated SUMOylation stimulates DNA–protein
cross-link repair in Arabidopsis

J 2023

SMC5/6 complex-mediated SUMOylation stimulates DNA–protein cross-link repair in Arabidopsis

TOMAŠTÍKOVÁ, Eva Dvořák, Klara PROCHAZKOVA, Fen YANG, Jitka JEMELKOVÁ, Andreas FINKE et. al.

Základní údaje

Originální název

SMC5/6 complex-mediated SUMOylation stimulates DNA–protein cross-link repair in Arabidopsis

Autoři

TOMAŠTÍKOVÁ, Eva Dvořák (garant), Klara PROCHAZKOVA, Fen YANG, Jitka JEMELKOVÁ (203 Česká republika, domácí), Andreas FINKE, Annika DORN, Mahmoud SAID, Holger PUCHTA a Ales PECINKA

Vydání

The Plant Cell, Oxford University Press, 2023, 1040-4651

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 11.600 v roce 2022

Kód RIV

RIV/00216224:14310/23:00131345

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1093/plcell/koad020

UT WoS

000933836600001

Klíčová slova anglicky

Arabidopsis; proteins; DNA damage repair; DNA-protein crosslinks; DPCs

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 21. 3. 2024 09:11, Mgr. Marie Šípková, DiS.

Anotace

V originále

DNA–protein cross-links (DPCs) are highly toxic DNA lesions consisting of proteins covalently attached to chromosomal DNA. Unrepaired DPCs physically block DNA replication and transcription. Three DPC repair pathways have been identified in Arabidopsis (Arabidopsis thaliana) to date: the endonucleolytic cleavage of DNA by the structure-specific endonuclease MUS81; proteolytic degradation of the crosslinked protein by the metalloprotease WSS1A; and cleavage of the cross-link phosphodiester bonds by the tyrosyl phosphodiesterases TDP1 and TDP2. Here we describe the evolutionary conserved STRUCTURAL MAINTENANCE OF CHROMOSOMEs SMC5/6 complex as a crucial component involved in DPC repair. We identified multiple alleles of the SMC5/6 complex core subunit gene SMC6B via a forward-directed genetic screen designed to identify the factors involved in the repair of DPCs induced by the cytidine analog zebularine. We monitored plant growth and cell death in response to DPC-inducing chemicals, which revealed that the SMC5/6 complex is essential for the repair of several types of DPCs. Genetic interaction and sensitivity assays showed that the SMC5/6 complex works in parallel to the endonucleolytic and proteolytic pathways. The repair of zebularine-induced DPCs was associated with SMC5/6-dependent SUMOylation of the damage sites. Thus, we present the SMC5/6 complex as an important factor in plant DPC repair.

Návaznosti

GA20-05095S, projekt VaV

Název: Úloha komplexu SMC5/6 a jeho interakčních partnerů v opravě poškozené DNA (Akronym: SMC5/6)

Investor: Grantová agentura ČR, Úloha komplexu SMC5/6 a jeho interakčních partnerů v opravě poškozené DNA

LM2018140, projekt VaV

Název: e-Infrastruktura CZ (Akronym: e-INFRA CZ)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, e-Infrastruktura CZ

Citovat

TOMAŠTÍKOVÁ, Eva Dvořák, Klara PROCHAZKOVA, Fen YANG, Jitka JEMELKOVÁ, Andreas FINKE, Annika DORN, Mahmoud SAID, Holger PUCHTA a Ales PECINKA. SMC5/6 complex-mediated SUMOylation stimulates DNA–protein cross-link repair in Arabidopsis. The Plant Cell. Oxford University Press, 2023, roč. 35, č. 5, s. 1532-1547. ISSN 1040-4651. Dostupné z: https://dx.doi.org/10.1093/plcell/koad020.

@article{2300338,
   author = {Tomaštíková, Eva Dvořák and Prochazkova, Klara and Yang, Fen and Jemelková, Jitka and Finke, Andreas and Dorn, Annika and Said, Mahmoud and Puchta, Holger and Pecinka, Ales},
   article_number = {5},
   doi = {http://dx.doi.org/10.1093/plcell/koad020},
   keywords = {Arabidopsis; proteins; DNA damage repair; DNA-protein crosslinks; DPCs},
   language = {eng},
   issn = {1040-4651},
   journal = {The Plant Cell},
   title = {SMC5/6 complex-mediated SUMOylation stimulates DNA–protein cross-link repair in Arabidopsis},
   url = {https://doi.org/10.1093/plcell/koad020},
   volume = {35},
   year = {2023}
}

TY  - JOUR
ID  - 2300338
AU  - Tomaštíková, Eva Dvořák - Prochazkova, Klara - Yang, Fen - Jemelková, Jitka - Finke, Andreas - Dorn, Annika - Said, Mahmoud - Puchta, Holger - Pecinka, Ales
PY  - 2023
TI  - SMC5/6 complex-mediated SUMOylation stimulates DNA–protein cross-link repair in Arabidopsis
JF  - The Plant Cell
VL  - 35
IS  - 5
SP  - 1532-1547
EP  - 1532-1547
PB  - Oxford University Press
SN  - 10404651
KW  - Arabidopsis
KW  - proteins
KW  - DNA damage repair
KW  - DNA-protein crosslinks
KW  - DPCs
UR  - https://doi.org/10.1093/plcell/koad020
N2  - DNA–protein cross-links (DPCs) are highly toxic DNA lesions consisting of proteins covalently attached to chromosomal DNA. Unrepaired DPCs physically block DNA replication and transcription. Three DPC repair pathways have been identified in Arabidopsis (Arabidopsis thaliana) to date: the endonucleolytic cleavage of DNA by the structure-specific endonuclease MUS81; proteolytic degradation of the crosslinked protein by the metalloprotease WSS1A; and cleavage of the cross-link phosphodiester bonds by the tyrosyl phosphodiesterases TDP1 and TDP2. Here we describe the evolutionary conserved STRUCTURAL MAINTENANCE OF CHROMOSOMEs SMC5/6 complex as a crucial component involved in DPC repair. We identified multiple alleles of the SMC5/6 complex core subunit gene SMC6B via a forward-directed genetic screen designed to identify the factors involved in the repair of DPCs induced by the cytidine analog zebularine. We monitored plant growth and cell death in response to DPC-inducing chemicals, which revealed that the SMC5/6 complex is essential for the repair of several types of DPCs. Genetic interaction and sensitivity assays showed that the SMC5/6 complex works in parallel to the endonucleolytic and proteolytic pathways. The repair of zebularine-induced DPCs was associated with SMC5/6-dependent SUMOylation of the damage sites. Thus, we present the SMC5/6 complex as an important factor in plant DPC repair.
ER  -

TOMAŠTÍKOVÁ, Eva Dvořák, Klara PROCHAZKOVA, Fen YANG, Jitka JEMELKOVÁ, Andreas FINKE, Annika DORN, Mahmoud SAID, Holger PUCHTA a Ales PECINKA. SMC5/6 complex-mediated SUMOylation stimulates DNA–protein cross-link repair in Arabidopsis. \textit{The Plant Cell}. Oxford University Press, 2023, roč.~35, č.~5, s.~1532-1547. ISSN~1040-4651. Dostupné z: https://dx.doi.org/10.1093/plcell/koad020.

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