Cerebral Malaria Model Applying Human Brain Organoids

J 2023

Cerebral Malaria Model Applying Human Brain Organoids

SILVA-PEDROSA, Rita, Jonas CAMPOS, Aline Marie FERNANDES, Miguel Joao FERREIRA DA SILVA, Carla CALCADA et. al.

Základní údaje

Originální název

Cerebral Malaria Model Applying Human Brain Organoids

Autoři

SILVA-PEDROSA, Rita (garant), Jonas CAMPOS, Aline Marie FERNANDES, Miguel Joao FERREIRA DA SILVA (620 Portugalsko, domácí), Carla CALCADA, Ana MAROTE, Olga MARTINHO, Maria Isabel VEIGA, Ligia R. RODRIGUES, Antonio Jose SALGADO a Pedro Eduardo FERREIRA

Vydání

Cells, MDPI, 2023, 2073-4409

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.000 v roce 2022

Kód RIV

RIV/00216224:14310/23:00131405

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.3390/cells12070984

UT WoS

000969330600001

Klíčová slova anglicky

cerebral malaria; brain organoids; transcriptome; human iPSCs; secretome; HBMEC activation

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 8. 2023 13:50, Mgr. Marie Šípková, DiS.

Anotace

V originále

Neural injuries in cerebral malaria patients are a significant cause of morbidity and mortality. Nevertheless, a comprehensive research approach to study this issue is lacking, so herein we propose an in vitro system to study human cerebral malaria using cellular approaches. Our first goal was to establish a cellular system to identify the molecular alterations in human brain vasculature cells that resemble the blood-brain barrier (BBB) in cerebral malaria (CM). Through transcriptomic analysis, we characterized specific gene expression profiles in human brain microvascular endothelial cells (HBMEC) activated by the Plasmodium falciparum parasites. We also suggest potential new genes related to parasitic activation. Then, we studied its impact at brain level after Plasmodium falciparum endothelial activation to gain a deeper understanding of the physiological mechanisms underlying CM. For that, the impact of HBMEC-P. falciparum-activated secretomes was evaluated in human brain organoids. Our results support the reliability of in vitro cellular models developed to mimic CM in several aspects. These systems can be of extreme importance to investigate the factors (parasitological and host) influencing CM, contributing to a molecular understanding of pathogenesis, brain injury, and dysfunction.

Citovat

SILVA-PEDROSA, Rita, Jonas CAMPOS, Aline Marie FERNANDES, Miguel Joao FERREIRA DA SILVA, Carla CALCADA, Ana MAROTE, Olga MARTINHO, Maria Isabel VEIGA, Ligia R. RODRIGUES, Antonio Jose SALGADO a Pedro Eduardo FERREIRA. Cerebral Malaria Model Applying Human Brain Organoids. Cells. MDPI, 2023, roč. 12, č. 7, s. 1-31. ISSN 2073-4409. Dostupné z: https://dx.doi.org/10.3390/cells12070984.

@article{2301841,
   author = {SilvaandPedrosa, Rita and Campos, Jonas and Fernandes, Aline Marie and Ferreira da Silva, Miguel Joao and Calcada, Carla and Marote, Ana and Martinho, Olga and Veiga, Maria Isabel and Rodrigues, Ligia R. and Salgado, Antonio Jose and Ferreira, Pedro Eduardo},
   article_number = {7},
   doi = {http://dx.doi.org/10.3390/cells12070984},
   keywords = {cerebral malaria; brain organoids; transcriptome; human iPSCs; secretome; HBMEC activation},
   language = {eng},
   issn = {2073-4409},
   journal = {Cells},
   title = {Cerebral Malaria Model Applying Human Brain Organoids},
   url = {https://doi.org/10.3390/cells12070984},
   volume = {12},
   year = {2023}
}

TY  - JOUR
ID  - 2301841
AU  - Silva-Pedrosa, Rita - Campos, Jonas - Fernandes, Aline Marie - Ferreira da Silva, Miguel Joao - Calcada, Carla - Marote, Ana - Martinho, Olga - Veiga, Maria Isabel - Rodrigues, Ligia R. - Salgado, Antonio Jose - Ferreira, Pedro Eduardo
PY  - 2023
TI  - Cerebral Malaria Model Applying Human Brain Organoids
JF  - Cells
VL  - 12
IS  - 7
SP  - 1-31
EP  - 1-31
PB  - MDPI
SN  - 20734409
KW  - cerebral malaria
KW  - brain organoids
KW  - transcriptome
KW  - human iPSCs
KW  - secretome
KW  - HBMEC activation
UR  - https://doi.org/10.3390/cells12070984
N2  - Neural injuries in cerebral malaria patients are a significant cause of morbidity and mortality. Nevertheless, a comprehensive research approach to study this issue is lacking, so herein we propose an in vitro system to study human cerebral malaria using cellular approaches. Our first goal was to establish a cellular system to identify the molecular alterations in human brain vasculature cells that resemble the blood-brain barrier (BBB) in cerebral malaria (CM). Through transcriptomic analysis, we characterized specific gene expression profiles in human brain microvascular endothelial cells (HBMEC) activated by the Plasmodium falciparum parasites. We also suggest potential new genes related to parasitic activation. Then, we studied its impact at brain level after Plasmodium falciparum endothelial activation to gain a deeper understanding of the physiological mechanisms underlying CM. For that, the impact of HBMEC-P. falciparum-activated secretomes was evaluated in human brain organoids. Our results support the reliability of in vitro cellular models developed to mimic CM in several aspects. These systems can be of extreme importance to investigate the factors (parasitological and host) influencing CM, contributing to a molecular understanding of pathogenesis, brain injury, and dysfunction.
ER  -

SILVA-PEDROSA, Rita, Jonas CAMPOS, Aline Marie FERNANDES, Miguel Joao FERREIRA DA SILVA, Carla CALCADA, Ana MAROTE, Olga MARTINHO, Maria Isabel VEIGA, Ligia R. RODRIGUES, Antonio Jose SALGADO a Pedro Eduardo FERREIRA. Cerebral Malaria Model Applying Human Brain Organoids. \textit{Cells}. MDPI, 2023, roč.~12, č.~7, s.~1-31. ISSN~2073-4409. Dostupné z: https://dx.doi.org/10.3390/cells12070984.

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