SILVA-PEDROSA, Rita, Jonas CAMPOS, Aline Marie FERNANDES, Miguel Joao FERREIRA DA SILVA, Carla CALCADA, Ana MAROTE, Olga MARTINHO, Maria Isabel VEIGA, Ligia R. RODRIGUES, Antonio Jose SALGADO a Pedro Eduardo FERREIRA. Cerebral Malaria Model Applying Human Brain Organoids. Cells. MDPI, 2023, roč. 12, č. 7, s. 1-31. ISSN 2073-4409. Dostupné z: https://dx.doi.org/10.3390/cells12070984. |
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@article{2301841, author = {SilvaandPedrosa, Rita and Campos, Jonas and Fernandes, Aline Marie and Ferreira da Silva, Miguel Joao and Calcada, Carla and Marote, Ana and Martinho, Olga and Veiga, Maria Isabel and Rodrigues, Ligia R. and Salgado, Antonio Jose and Ferreira, Pedro Eduardo}, article_number = {7}, doi = {http://dx.doi.org/10.3390/cells12070984}, keywords = {cerebral malaria; brain organoids; transcriptome; human iPSCs; secretome; HBMEC activation}, language = {eng}, issn = {2073-4409}, journal = {Cells}, title = {Cerebral Malaria Model Applying Human Brain Organoids}, url = {https://doi.org/10.3390/cells12070984}, volume = {12}, year = {2023} }
TY - JOUR ID - 2301841 AU - Silva-Pedrosa, Rita - Campos, Jonas - Fernandes, Aline Marie - Ferreira da Silva, Miguel Joao - Calcada, Carla - Marote, Ana - Martinho, Olga - Veiga, Maria Isabel - Rodrigues, Ligia R. - Salgado, Antonio Jose - Ferreira, Pedro Eduardo PY - 2023 TI - Cerebral Malaria Model Applying Human Brain Organoids JF - Cells VL - 12 IS - 7 SP - 1-31 EP - 1-31 PB - MDPI SN - 20734409 KW - cerebral malaria KW - brain organoids KW - transcriptome KW - human iPSCs KW - secretome KW - HBMEC activation UR - https://doi.org/10.3390/cells12070984 N2 - Neural injuries in cerebral malaria patients are a significant cause of morbidity and mortality. Nevertheless, a comprehensive research approach to study this issue is lacking, so herein we propose an in vitro system to study human cerebral malaria using cellular approaches. Our first goal was to establish a cellular system to identify the molecular alterations in human brain vasculature cells that resemble the blood-brain barrier (BBB) in cerebral malaria (CM). Through transcriptomic analysis, we characterized specific gene expression profiles in human brain microvascular endothelial cells (HBMEC) activated by the Plasmodium falciparum parasites. We also suggest potential new genes related to parasitic activation. Then, we studied its impact at brain level after Plasmodium falciparum endothelial activation to gain a deeper understanding of the physiological mechanisms underlying CM. For that, the impact of HBMEC-P. falciparum-activated secretomes was evaluated in human brain organoids. Our results support the reliability of in vitro cellular models developed to mimic CM in several aspects. These systems can be of extreme importance to investigate the factors (parasitological and host) influencing CM, contributing to a molecular understanding of pathogenesis, brain injury, and dysfunction. ER -
SILVA-PEDROSA, Rita, Jonas CAMPOS, Aline Marie FERNANDES, Miguel Joao FERREIRA DA SILVA, Carla CALCADA, Ana MAROTE, Olga MARTINHO, Maria Isabel VEIGA, Ligia R. RODRIGUES, Antonio Jose SALGADO a Pedro Eduardo FERREIRA. Cerebral Malaria Model Applying Human Brain Organoids. \textit{Cells}. MDPI, 2023, roč.~12, č.~7, s.~1-31. ISSN~2073-4409. Dostupné z: https://dx.doi.org/10.3390/cells12070984.
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