Mobility shift affinity capillary electrophoresis using a collagen suspension: Method development for the study of protein binding properties

HARTUNG, Sophie, Christin SCHELLER, Taťána BRŽEZICKÁ a Wätzig HERMANN. Mobility shift affinity capillary electrophoresis using a collagen suspension: Method development for the study of protein binding properties. In 51st International Symposium on High Performance Liquid Phase Separations and Related Techniques, HPLC 2023. 2023.

Základní údaje

• Originální název: Mobility shift affinity capillary electrophoresis using a collagen suspension: Method development for the study of protein binding properties
• Autoři: HARTUNG, Sophie, Christin SCHELLER, Taťána BRŽEZICKÁ a Wätzig HERMANN.
• Vydání: 51st International Symposium on High Performance Liquid Phase Separations and Related Techniques, HPLC 2023, 2023.

Další údaje

• Originální jazyk: angličtina
• Typ výsledku: Konferenční abstrakt
• Obor: 10609 Biochemical research methods
• Stát vydavatele: Německo
• Utajení: není předmětem státního či obchodního tajemství
• WWW: URL URL
• Příznaky: Mezinárodní význam, Recenzováno

Anotace

Mobility shift affinity capillary electrophoresis is an approach to study interactions of drugs with biomaterials. This technology can help to better understand the function and role of proteins in the human body and improves the development of drugs and medical devices. Therefore, various concentrations of ligand are dissolved in the BGE and an analyte and a non-interacting mobility marker are injected into the capillary. During electrophoresis, a ligand's presence can change the analyte's effective electrophoretic mobility. Thus, by fitting the data against a model function through non-linear regression, the strength of the interaction can be estimated. [1] This project aims to investigate the binding properties of proteins with a collagen product constructed for wound healing to find out about its biocompatibility. As a BGE, a phosphate buffer with pH = 7.4 was chosen to mimic the natural conditions of the human body. Because of the poor solubility of collagen in a neutral medium, a procedure was established to make parts of the collagen product suspendable which was achieved by grinding and freeze-drying. Subsequently, a robust method will be developed to measure different proteins reproducibly with and without the presence of collagen. The development of such a method is challenging since differences in migration time and peak shape can occur due to adsorptive capillary effects. Therefore, an LPA-coated capillary (75 μm ID) is used to reduce protein adhesion. Another important aspect is, that the BGE containing collagen is a suspension and this results in an increase in current strength. Thus, we chose constant power as separation mode. The addition of large amounts of collagen changes the viscosity of the BGE and must be considered in later calculations. It should also be investigated whether the mobility marker is suitable and does not interact with other compounds in the capillary. Finally, mobility shift affinity capillary electrophoresis with collagen at various concentrations as ligand, the respective proteins, and a non-interacting marker will be carried out and evaluated.