Enrichment of human nasopharyngeal bacteriome with bacteria
from dust after short-term exposure to indoor environment: a
pilot study

J 2023

Enrichment of human nasopharyngeal bacteriome with bacteria from dust after short-term exposure to indoor environment: a pilot study

KONEČNÁ, Eva, Petra VÍDEŇSKÁ, Lucie BUREŠOVÁ, Milan URÍK, Soňa SMETANOVÁ et. al.

Základní údaje

Originální název

Enrichment of human nasopharyngeal bacteriome with bacteria from dust after short-term exposure to indoor environment: a pilot study

Autoři

KONEČNÁ, Eva (203 Česká republika, domácí), Petra VÍDEŇSKÁ (203 Česká republika, domácí), Lucie BUREŠOVÁ (203 Česká republika, domácí), Milan URÍK (703 Slovensko, domácí), Soňa SMETANOVÁ (203 Česká republika, domácí), Stanislav SMATANA (703 Slovensko, domácí), Roman PROKEŠ (203 Česká republika, domácí), Barbara LÁNÍČKOVÁ (203 Česká republika, domácí), Eva BUDINSKÁ (703 Slovensko, domácí), Jana KLÁNOVÁ (203 Česká republika, domácí) a Petra BOŘILOVÁ LINHARTOVÁ (203 Česká republika, garant, domácí)

Vydání

BMC Microbiology, London, BioMed Central, 2023, 1471-2180

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.200 v roce 2022

Kód RIV

RIV/00216224:14310/23:00131440

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1186/s12866-023-02951-5

UT WoS

001040951200002

Klíčová slova anglicky

Bacteriome; Dust; Nasopharynx; Household; Hospital; Sequencing; 16S rRNA; Exposure; Indoor environment

Štítky

14110319, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 8. 3. 2024 11:56, Mgr. Marie Šípková, DiS.

Anotace

V originále

BackgroundIndoor dust particles are an everyday source of human exposure to microorganisms and their inhalation may directly affect the microbiota of the respiratory tract. We aimed to characterize the changes in human nasopharyngeal bacteriome after short-term exposure to indoor (workplace) environments.MethodsIn this pilot study, nasopharyngeal swabs were taken from 22 participants in the morning and after 8 h of their presence at the workplace. At the same time points, indoor dust samples were collected from the participants' households (16 from flats and 6 from houses) and workplaces (8 from a maternity hospital - NEO, 6 from a pediatric hospital - ENT, and 8 from a research center - RCX). 16S rRNA sequencing analysis was performed on these human and environmental matrices.ResultsStaphylococcus and Corynebacterium were the most abundant genera in both indoor dust and nasopharyngeal samples. The analysis indicated lower bacterial diversity in indoor dust samples from flats compared to houses, NEO, ENT, and RCX (p < 0.05). Participants working in the NEO had the highest nasopharyngeal bacterial diversity of all groups (p < 0.05). After 8 h of exposure to the workplace environment, enrichment of the nasopharynx with several new bacterial genera present in the indoor dust was observed in 76% of study participants; however, no significant changes were observed at the level of the nasopharyngeal bacterial diversity (p > 0.05, Shannon index). These "enriching" bacterial genera overlapped between the hospital workplaces - NEO and ENT but differed from those in the research center - RCX.ConclusionsThe results suggest that although the composition of nasopharyngeal bacteriome is relatively stable during the day. Short-term exposure to the indoor environment can result in the enrichment of the nasopharynx with bacterial DNA from indoor dust; the bacterial composition, however, varies by the indoor workplace environment.

Návaznosti

EF15_003/0000469, projekt VaV

Název: Cetocoen Plus

EF17_043/0009632, projekt VaV

Název: CETOCOEN Excellence

EF19_073/0016943, projekt VaV

Název: Interní grantová agentura Masarykovy univerzity

GA22-32743S, projekt VaV

Název: Propojení složení chemických látek a mikrobiomu v domácím prachu s environmentálními parametry

Investor: Grantová agentura ČR, Propojení složení chemických látek a mikrobiomu v domácím prachu s environmentálními parametry

LM2018132, projekt VaV

Název: Národní centrum lékařské genomiky (Akronym: NCLG)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Národní centrum lékařské genomiky

LM2023069, projekt VaV

Název: Výzkumná infrastruktura RECETOX

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Výzkumná infrastruktura RECETOX

Přiložené soubory

2302818.pdf

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Citovat

KONEČNÁ, Eva, Petra VÍDEŇSKÁ, Lucie BUREŠOVÁ, Milan URÍK, Soňa SMETANOVÁ, Stanislav SMATANA, Roman PROKEŠ, Barbara LÁNÍČKOVÁ, Eva BUDINSKÁ, Jana KLÁNOVÁ a Petra BOŘILOVÁ LINHARTOVÁ. Enrichment of human nasopharyngeal bacteriome with bacteria from dust after short-term exposure to indoor environment: a pilot study. BMC Microbiology. London: BioMed Central, 2023, roč. 23, č. 1, s. 1-16. ISSN 1471-2180. Dostupné z: https://dx.doi.org/10.1186/s12866-023-02951-5.

@article{2302818,
   author = {Konečná, Eva and Vídeňská, Petra and Burešová, Lucie and Urík, Milan and Smetanová, Soňa and Smatana, Stanislav and Prokeš, Roman and Láníčková, Barbara and Budinská, Eva and Klánová, Jana and Bořilová Linhartová, Petra},
   article_location = {London},
   article_number = {1},
   doi = {http://dx.doi.org/10.1186/s12866-023-02951-5},
   keywords = {Bacteriome; Dust; Nasopharynx; Household; Hospital; Sequencing; 16S rRNA; Exposure; Indoor environment},
   language = {eng},
   issn = {1471-2180},
   journal = {BMC Microbiology},
   title = {Enrichment of human nasopharyngeal bacteriome with bacteria from dust after short-term exposure to indoor environment: a pilot study},
   url = {https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-023-02951-5},
   volume = {23},
   year = {2023}
}

TY  - JOUR
ID  - 2302818
AU  - Konečná, Eva - Vídeňská, Petra - Burešová, Lucie - Urík, Milan - Smetanová, Soňa - Smatana, Stanislav - Prokeš, Roman - Láníčková, Barbara - Budinská, Eva - Klánová, Jana - Bořilová Linhartová, Petra
PY  - 2023
TI  - Enrichment of human nasopharyngeal bacteriome with bacteria from dust after short-term exposure to indoor environment: a pilot study
JF  - BMC Microbiology
VL  - 23
IS  - 1
SP  - 1-16
EP  - 1-16
PB  - BioMed Central
SN  - 14712180
KW  - Bacteriome
KW  - Dust
KW  - Nasopharynx
KW  - Household
KW  - Hospital
KW  - Sequencing
KW  - 16S rRNA
KW  - Exposure
KW  - Indoor environment
UR  - https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-023-02951-5
N2  - BackgroundIndoor dust particles are an everyday source of human exposure to microorganisms and their inhalation may directly affect the microbiota of the respiratory tract. We aimed to characterize the changes in human nasopharyngeal bacteriome after short-term exposure to indoor (workplace) environments.MethodsIn this pilot study, nasopharyngeal swabs were taken from 22 participants in the morning and after 8 h of their presence at the workplace. At the same time points, indoor dust samples were collected from the participants' households (16 from flats and 6 from houses) and workplaces (8 from a maternity hospital - NEO, 6 from a pediatric hospital - ENT, and 8 from a research center - RCX). 16S rRNA sequencing analysis was performed on these human and environmental matrices.ResultsStaphylococcus and Corynebacterium were the most abundant genera in both indoor dust and nasopharyngeal samples. The analysis indicated lower bacterial diversity in indoor dust samples from flats compared to houses, NEO, ENT, and RCX (p < 0.05). Participants working in the NEO had the highest nasopharyngeal bacterial diversity of all groups (p < 0.05). After 8 h of exposure to the workplace environment, enrichment of the nasopharynx with several new bacterial genera present in the indoor dust was observed in 76% of study participants; however, no significant changes were observed at the level of the nasopharyngeal bacterial diversity (p > 0.05, Shannon index). These "enriching" bacterial genera overlapped between the hospital workplaces - NEO and ENT but differed from those in the research center - RCX.ConclusionsThe results suggest that although the composition of nasopharyngeal bacteriome is relatively stable during the day. Short-term exposure to the indoor environment can result in the enrichment of the nasopharynx with bacterial DNA from indoor dust; the bacterial composition, however, varies by the indoor workplace environment.
ER  -

KONEČNÁ, Eva, Petra VÍDEŇSKÁ, Lucie BUREŠOVÁ, Milan URÍK, Soňa SMETANOVÁ, Stanislav SMATANA, Roman PROKEŠ, Barbara LÁNÍČKOVÁ, Eva BUDINSKÁ, Jana KLÁNOVÁ a Petra BOŘILOVÁ LINHARTOVÁ. Enrichment of human nasopharyngeal bacteriome with bacteria from dust after short-term exposure to indoor environment: a pilot study. \textit{BMC Microbiology}. London: BioMed Central, 2023, roč.~23, č.~1, s.~1-16. ISSN~1471-2180. Dostupné z: https://dx.doi.org/10.1186/s12866-023-02951-5.

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