J 2023

Capillary Electrophoresis with Interchangeable Cartridges for Versatile and Automated Analyses of Dried Blood Spot Samples

DVOŘÁK, Miloš, Ondrej MORAVČÍK a Pavel KUBÁŇ

Základní údaje

Originální název

Capillary Electrophoresis with Interchangeable Cartridges for Versatile and Automated Analyses of Dried Blood Spot Samples

Autoři

DVOŘÁK, Miloš, Ondrej MORAVČÍK (703 Slovensko, domácí) a Pavel KUBÁŇ (203 Česká republika, garant)

Vydání

Analytical chemistry, American Chemical Society, 2023, 0003-2700

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Impakt faktor

Impact factor: 7.400 v roce 2022

Kód RIV

RIV/00216224:14310/23:00131500

Organizační jednotka

Přírodovědecká fakulta

DOI

UT WoS

001039692100001

Klíčová slova anglicky

Anatomy; Calibration; Computer simulations; Genetics; Solvents

Štítky

Příznaky

Mezinárodní význam, Recenzováno
Změněno: 25. 8. 2023 11:54, Mgr. Marie Šípková, DiS.

Anotace

V originále

A novel concept forhighly versatile automated analyses of driedblood spot (DBS) samples by commercial capillary electrophoresis (CE)is presented. Two interchangeable CE cartridges with different fused-silicacapillaries were used for the DBS elutions and the DBS eluate analyses,respectively. The application of one CE cartridge with a wide-borecapillary reduced DBS processing times to a minimum (1-2 minper sample) while fitting the other CE cartridge with a narrow-borecapillary served for highly efficient CE analyses. A comprehensiveinvestigation of major variables affecting liquid handling in CE (capillarylength, internal diameter, and temperature) was carried out with theaim of optimizing both procedures and enabling their maximum flexibility.The application of two CE cartridges also enabled the employment ofCE detectors with different instrumental set-ups and/or principlesas was demonstrated by the optical detection of nonsteroidal anti-inflammatorydrugs (NSAIDs) and the conductivity detection of amino acids (AAs).The presented methods were optimized for the automated CE analysesof 36 DBS samples formed by a volumetric collection of 5 & mu;Lof capillary blood onto Whatman 903 discs and processed by directin-vial elution using the CE instrument. The precision of liquid transfersfor the automated DBS elutions was better than 0.9% and the precisionof CE analyses did not exceed 5.1 and 12.3% for the determinationof NSAIDs and AAs, respectively. Both methods were linear (R (2) & GE; 0.996) over the therapeutic (NSAIDs)and the endogenous (AAs) concentration ranges, had limits of quantificationbelow the typical analyte concentrations in human blood, and achievedsample throughputs of more than 6 DBSs per hour.