Spatial positioning of preimplantation mouse embryo cells is
regulated by mTORC1 and m(7)G-cap-dependent translation at the
8-to 16-cell transition

J 2023

Spatial positioning of preimplantation mouse embryo cells is regulated by mTORC1 and m(7)G-cap-dependent translation at the 8-to 16-cell transition

GAHUROVÁ, Lenka, Jana TOMÁNKOVÁ, Pavla ČERNÁ, Pablo BORA, Michaela KUBÍČKOVÁ et. al.

Základní údaje

Originální název

Spatial positioning of preimplantation mouse embryo cells is regulated by mTORC1 and m(7)G-cap-dependent translation at the 8-to 16-cell transition

Autoři

GAHUROVÁ, Lenka (203 Česká republika), Jana TOMÁNKOVÁ (203 Česká republika), Pavla ČERNÁ (203 Česká republika), Pablo BORA (203 Česká republika), Michaela KUBÍČKOVÁ (203 Česká republika), Giorgio VIRNICCHI (203 Česká republika), Kristina KOVAŘOVICOVÁ, David POTĚŠIL (203 Česká republika, domácí), Pavel HRUŠKA (203 Česká republika, domácí), Zbyněk ZDRÁHAL (203 Česká republika, domácí), Martin ANGER, Andrej ŠUŠOT (203 Česká republika) a Alexander W. BRUCE (203 Česká republika, garant)

Vydání

OPEN BIOLOGY, ENGLAND, ROYAL SOC, 2023, 2046-2441

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

fulltext

Impakt faktor

Impact factor: 5.800 v roce 2022

Kód RIV

RIV/00216224:14740/23:00131564

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1098/rsob.230081

UT WoS

001044130500002

Klíčová slova anglicky

mTOR;mTORC1;EIF4EBP1;4EBP1;TOP-motif;preimplantation mouse embryo;cell fate;inner cell mass;ICM and cell positioning

Štítky

CF PROT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 11. 2024 21:22, Ing. Martina Blahová

Anotace

V originále

Preimplantation mouse embryo development involves temporal-spatial specification and segregation of three blastocyst cell lineages: trophectoderm, primitive endoderm and epiblast. Spatial separation of the outer-trophectoderm lineage from the two other inner-cell-mass (ICM) lineages starts with the 8- to 16-cell transition and concludes at the 32-cell stages. Accordingly, the ICM is derived from primary and secondary contributed cells; with debated relative EPI versus PrE potencies. We report generation of primary but not secondary ICM populations is highly dependent on temporal activation of mammalian target of Rapamycin (mTOR) during 8-cell stage M-phase entry, mediated via regulation of the 7-methylguanosine-cap (m(7)G-cap)-binding initiation complex (EIF4F) and linked to translation of mRNAs containing 5 & PRIME; UTR terminal oligopyrimidine (TOP-) sequence motifs, as knockdown of identified TOP-like motif transcripts impairs generation of primary ICM founders. However, mTOR inhibition-induced ICM cell number deficits in early blastocysts can be compensated by the late blastocyst stage, after inhibitor withdrawal; compensation likely initiated at the 32-cell stage when supernumerary outer cells exhibit molecular characteristics of inner cells. These data identify a novel mechanism specifically governing initial spatial segregation of mouse embryo blastomeres, that is distinct from those directing subsequent inner cell formation, contributing to germane segregation of late blastocyst lineages.

Návaznosti

LM2018140, projekt VaV

Název: e-Infrastruktura CZ (Akronym: e-INFRA CZ)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, e-Infrastruktura CZ

90242, velká výzkumná infrastruktura

Název: CIISB III

Citovat

GAHUROVÁ, Lenka, Jana TOMÁNKOVÁ, Pavla ČERNÁ, Pablo BORA, Michaela KUBÍČKOVÁ, Giorgio VIRNICCHI, Kristina KOVAŘOVICOVÁ, David POTĚŠIL, Pavel HRUŠKA, Zbyněk ZDRÁHAL, Martin ANGER, Andrej ŠUŠOT a Alexander W. BRUCE. Spatial positioning of preimplantation mouse embryo cells is regulated by mTORC1 and m(7)G-cap-dependent translation at the 8-to 16-cell transition. OPEN BIOLOGY. ENGLAND: ROYAL SOC, 2023, roč. 13, č. 8, s. 1-19. ISSN 2046-2441. Dostupné z: https://dx.doi.org/10.1098/rsob.230081.

@article{2305479,
   author = {Gahurová, Lenka and Tománková, Jana and Černá, Pavla and Bora, Pablo and Kubíčková, Michaela and Virnicchi, Giorgio and Kovařovicová, Kristina and Potěšil, David and Hruška, Pavel and Zdráhal, Zbyněk and Anger, Martin and Šušot, Andrej and Bruce, Alexander W.},
   article_location = {ENGLAND},
   article_number = {8},
   doi = {http://dx.doi.org/10.1098/rsob.230081},
   keywords = {mTOR;mTORC1;EIF4EBP1;4EBP1;TOP-motif;preimplantation mouse embryo;cell fate;inner cell mass;ICM and cell positioning},
   language = {eng},
   issn = {2046-2441},
   journal = {OPEN BIOLOGY},
   title = {Spatial positioning of preimplantation mouse embryo cells is regulated by mTORC1 and m(7)G-cap-dependent translation at the 8-to 16-cell transition},
   url = {https://royalsocietypublishing.org/doi/epdf/10.1098/rsob.230081},
   volume = {13},
   year = {2023}
}

TY  - JOUR
ID  - 2305479
AU  - Gahurová, Lenka - Tománková, Jana - Černá, Pavla - Bora, Pablo - Kubíčková, Michaela - Virnicchi, Giorgio - Kovařovicová, Kristina - Potěšil, David - Hruška, Pavel - Zdráhal, Zbyněk - Anger, Martin - Šušot, Andrej - Bruce, Alexander W.
PY  - 2023
TI  - Spatial positioning of preimplantation mouse embryo cells is regulated by mTORC1 and m(7)G-cap-dependent translation at the 8-to 16-cell transition
JF  - OPEN BIOLOGY
VL  - 13
IS  - 8
SP  - 1-19
EP  - 1-19
PB  - ROYAL SOC
SN  - 20462441
KW  - mTOR;mTORC1;EIF4EBP1;4EBP1;TOP-motif;preimplantation mouse embryo;cell fate;inner cell mass;ICM and cell positioning
UR  - https://royalsocietypublishing.org/doi/epdf/10.1098/rsob.230081
N2  - Preimplantation mouse embryo development involves temporal-spatial specification and segregation of three blastocyst cell lineages: trophectoderm, primitive endoderm and epiblast. Spatial separation of the outer-trophectoderm lineage from the two other inner-cell-mass (ICM) lineages starts with the 8- to 16-cell transition and concludes at the 32-cell stages. Accordingly, the ICM is derived from primary and secondary contributed cells; with debated relative EPI versus PrE potencies. We report generation of primary but not secondary ICM populations is highly dependent on temporal activation of mammalian target of Rapamycin (mTOR) during 8-cell stage M-phase entry, mediated via regulation of the 7-methylguanosine-cap (m(7)G-cap)-binding initiation complex (EIF4F) and linked to translation of mRNAs containing 5 & PRIME; UTR terminal oligopyrimidine (TOP-) sequence motifs, as knockdown of identified TOP-like motif transcripts impairs generation of primary ICM founders. However, mTOR inhibition-induced ICM cell number deficits in early blastocysts can be compensated by the late blastocyst stage, after inhibitor withdrawal; compensation likely initiated at the 32-cell stage when supernumerary outer cells exhibit molecular characteristics of inner cells. These data identify a novel mechanism specifically governing initial spatial segregation of mouse embryo blastomeres, that is distinct from those directing subsequent inner cell formation, contributing to germane segregation of late blastocyst lineages.
ER  -

GAHUROVÁ, Lenka, Jana TOMÁNKOVÁ, Pavla ČERNÁ, Pablo BORA, Michaela KUBÍČKOVÁ, Giorgio VIRNICCHI, Kristina KOVAŘOVICOVÁ, David POTĚŠIL, Pavel HRUŠKA, Zbyněk ZDRÁHAL, Martin ANGER, Andrej ŠUŠOT a Alexander W. BRUCE. Spatial positioning of preimplantation mouse embryo cells is regulated by mTORC1 and m(7)G-cap-dependent translation at the 8-to 16-cell transition. \textit{OPEN BIOLOGY}. ENGLAND: ROYAL SOC, 2023, roč.~13, č.~8, s.~1-19. ISSN~2046-2441. Dostupné z: https://dx.doi.org/10.1098/rsob.230081.

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