Characteristics of N-Glycosylation and Its Impact on the
Molecular Behavior of Lupinus angustifolius ?-Conglutin

J 2023

Characteristics of N-Glycosylation and Its Impact on the Molecular Behavior of Lupinus angustifolius ?-Conglutin

CZUBINSKI, Jaroslaw, Erika LATTOVÁ, Zbyněk ZDRÁHAL a Richard STRASSER

Základní údaje

Originální název

Characteristics of N-Glycosylation and Its Impact on the Molecular Behavior of Lupinus angustifolius ?-Conglutin

Autoři

CZUBINSKI, Jaroslaw (616 Polsko), Erika LATTOVÁ (703 Slovensko, garant, domácí), Zbyněk ZDRÁHAL (203 Česká republika, domácí) a Richard STRASSER (40 Rakousko)

Vydání

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, UNITED STATES, AMER CHEMICAL SOC, 2023, 0021-8561

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

40500 4.5 Other agricultural sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

fulltext

Impakt faktor

Impact factor: 6.100 v roce 2022

Kód RIV

RIV/00216224:14740/23:00131565

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1021/acs.jafc.3c00727

UT WoS

000986537100001

Klíčová slova anglicky

lupin seed;gamma-conglutin;N-glycosylation;mass spectrometry;post-translational modification;thermal stability

Štítky

CF PROT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 6. 4. 2024 13:15, Mgr. Eva Dubská

Anotace

V originále

a-Conglutin, a lupin seed protein, is an intriguing protein both in terms of the complexity of its molecular structure and a broad spectrum of unique health-promoting properties manifested in animal and human trials. Moreover, this protein is an evolutionary cornerstone whose physiological significance for the plant has not been determined yet. Herein, a comprehensive characterization of a-conglutin glycosylation is presented and includes the identification of the N-glycan-bearing site, the qualitative and quantitative composition of glycan-building saccharides, as well as the effect of oligosaccharide removal on structural and thermal stability. The obtained results indicate the presence of glycans belonging to different classes attached to the Asn98 residue. In addition, the detachment of the oligosaccharide significantly affects secondary structure composition, which disturbs the oligomerization process. The structural changes were also reflected in biophysical parameters, i.e., at a pH value of 4.5, an increase in a-conglutin thermal stability was observed for the deglycosylated monomeric form. Collectively, the presented results provide evidence of the high complexity of the post-translational maturation and suggest the possibility of a functional effect that glycosylation might have on a-conglutin structure integrity.

Návaznosti

EF16_026/0008446, projekt VaV

Název: Integrace signálu a epigenetické reprogramování pro produktivitu rostlin

LM2023042, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CIISB - Česká infrastruktura pro integrativní strukturní biologii

90242, velká výzkumná infrastruktura

Název: CIISB III

Citovat

CZUBINSKI, Jaroslaw, Erika LATTOVÁ, Zbyněk ZDRÁHAL a Richard STRASSER. Characteristics of N-Glycosylation and Its Impact on the Molecular Behavior of Lupinus angustifolius ?-Conglutin. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. UNITED STATES: AMER CHEMICAL SOC, 2023, roč. 71, č. 19, s. 7359-7369. ISSN 0021-8561. Dostupné z: https://dx.doi.org/10.1021/acs.jafc.3c00727.

@article{2305480,
   author = {Czubinski, Jaroslaw and Lattová, Erika and Zdráhal, Zbyněk and Strasser, Richard},
   article_location = {UNITED STATES},
   article_number = {19},
   doi = {http://dx.doi.org/10.1021/acs.jafc.3c00727},
   keywords = {lupin seed;gamma-conglutin;N-glycosylation;mass spectrometry;post-translational modification;thermal stability},
   language = {eng},
   issn = {0021-8561},
   journal = {JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY},
   title = {Characteristics of N-Glycosylation and Its Impact on the Molecular Behavior of Lupinus angustifolius ?-Conglutin},
   url = {https://pubs.acs.org/doi/epdf/10.1021/acs.jafc.3c00727},
   volume = {71},
   year = {2023}
}

TY  - JOUR
ID  - 2305480
AU  - Czubinski, Jaroslaw - Lattová, Erika - Zdráhal, Zbyněk - Strasser, Richard
PY  - 2023
TI  - Characteristics of N-Glycosylation and Its Impact on the Molecular Behavior of Lupinus angustifolius ?-Conglutin
JF  - JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
VL  - 71
IS  - 19
SP  - 7359-7369
EP  - 7359-7369
PB  - AMER CHEMICAL SOC
SN  - 00218561
KW  - lupin seed;gamma-conglutin;N-glycosylation;mass spectrometry;post-translational modification;thermal stability
UR  - https://pubs.acs.org/doi/epdf/10.1021/acs.jafc.3c00727
N2  - a-Conglutin, a lupin seed protein, is an intriguing protein both in terms of the complexity of its molecular structure and a broad spectrum of unique health-promoting properties manifested in animal and human trials. Moreover, this protein is an evolutionary cornerstone whose physiological significance for the plant has not been determined yet. Herein, a comprehensive characterization of a-conglutin glycosylation is presented and includes the identification of the N-glycan-bearing site, the qualitative and quantitative composition of glycan-building saccharides, as well as the effect of oligosaccharide removal on structural and thermal stability. The obtained results indicate the presence of glycans belonging to different classes attached to the Asn98 residue. In addition, the detachment of the oligosaccharide significantly affects secondary structure composition, which disturbs the oligomerization process. The structural changes were also reflected in biophysical parameters, i.e., at a pH value of 4.5, an increase in a-conglutin thermal stability was observed for the deglycosylated monomeric form. Collectively, the presented results provide evidence of the high complexity of the post-translational maturation and suggest the possibility of a functional effect that glycosylation might have on a-conglutin structure integrity.
ER  -

CZUBINSKI, Jaroslaw, Erika LATTOVÁ, Zbyněk ZDRÁHAL a Richard STRASSER. Characteristics of N-Glycosylation and Its Impact on the Molecular Behavior of Lupinus angustifolius ?-Conglutin. \textit{JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY}. UNITED STATES: AMER CHEMICAL SOC, 2023, roč.~71, č.~19, s.~7359-7369. ISSN~0021-8561. Dostupné z: https://dx.doi.org/10.1021/acs.jafc.3c00727.

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