Autonomous capillary electrophoresis processing and analysis of
dried blood spots for high-throughput determination of uric
acid

J 2023

Autonomous capillary electrophoresis processing and analysis of dried blood spots for high-throughput determination of uric acid

MORAVČÍK, Ondrej, Miloš DVOŘÁK a Pavel KUBÁŇ

Základní údaje

Originální název

Autonomous capillary electrophoresis processing and analysis of dried blood spots for high-throughput determination of uric acid

Autoři

MORAVČÍK, Ondrej (703 Slovensko, domácí), Miloš DVOŘÁK a Pavel KUBÁŇ (garant)

Vydání

Analytica Chimica Acta, Amsterdam, Elsevier, 2023, 0003-2670

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.200 v roce 2022

Kód RIV

RIV/00216224:14310/23:00131620

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.aca.2023.341390

UT WoS

001002501500001

Klíčová slova anglicky

Automation; Dried blood spot; Capillary electrophoresis; Uric acid

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 7. 9. 2023 10:38, Mgr. Marie Šípková, DiS.

Anotace

V originále

A new set-up for fully autonomous and high-throughput capillary electrophoresis (CE) analyses of dried blood spot (DBS) samples is presented. The DBS samples were prepared by collecting exactly 5 μL of capillary blood from a finger-prick onto a pre-punched DBS disc in a disposable plastic CE vial and by in-vial blood drying. The vials with the DBS samples were then loaded into a commercial CE instrument for a fully unmanned sample processing and analysis. A fused-silica capillary of the CE instrument was first used for the transfer of 100 μL of elution solvent to each vial, in-vial DBS elution, and in-vial eluate homogenization. The same capillary was also used for at-line injection, separation, and selective analysis of the resulting eluates. Novel CE sequences were tailor-programmed for consecutive processing and analyses of multiple DBSs, which facilitated a fully autonomous determination of uric acid with a throughput of 240 DBS samples per day (24 h). The presented analytical protocol (using 100 μm i. d./30 cm capillary; 30 mM 2-(N-morpholino)-ethanesulfonic acid, 30 mM l-histidine, and 30 μM cetyltrimethylammonium bromide background electrolyte solution; and UV detection at 292 nm) provided excellent precision at endogenous and spiked uric acid concentrations with RSD values of peak areas below 3.2%. Calibration curves were linear over the 33.3 − 1200 μM range (R2 better than 0.998), limits of detection and quantification in the original capillary blood were 10 and 33.3 μM, respectively, and were well below the uric acid clinical range (140–420 μM). The stability of uric acid in DBS samples stored at laboratory temperature for up to 2 months was also excellent demonstrating less than a 4.2% decrease in uric acid concentrations. The actual set-up might thus be highly attractive for clinical subjects and laboratories because it is minimally invasive and requires minimum intervention from laboratory staff.

Citovat

MORAVČÍK, Ondrej, Miloš DVOŘÁK a Pavel KUBÁŇ. Autonomous capillary electrophoresis processing and analysis of dried blood spots for high-throughput determination of uric acid. Analytica Chimica Acta. Amsterdam: Elsevier, 2023, roč. 1267, August, s. 1-8. ISSN 0003-2670. Dostupné z: https://dx.doi.org/10.1016/j.aca.2023.341390.

@article{2307637,
   author = {Moravčík, Ondrej and Dvořák, Miloš and Kubáň, Pavel},
   article_location = {Amsterdam},
   article_number = {August},
   doi = {http://dx.doi.org/10.1016/j.aca.2023.341390},
   keywords = {Automation; Dried blood spot; Capillary electrophoresis; Uric acid},
   language = {eng},
   issn = {0003-2670},
   journal = {Analytica Chimica Acta},
   title = {Autonomous capillary electrophoresis processing and analysis of dried blood spots for high-throughput determination of uric acid},
   url = {https://doi.org/10.1016/j.aca.2023.341390},
   volume = {1267},
   year = {2023}
}

TY  - JOUR
ID  - 2307637
AU  - Moravčík, Ondrej - Dvořák, Miloš - Kubáň, Pavel
PY  - 2023
TI  - Autonomous capillary electrophoresis processing and analysis of dried blood spots for high-throughput determination of uric acid
JF  - Analytica Chimica Acta
VL  - 1267
IS  - August
SP  - 1-8
EP  - 1-8
PB  - Elsevier
SN  - 00032670
KW  - Automation
KW  - Dried blood spot
KW  - Capillary electrophoresis
KW  - Uric acid
UR  - https://doi.org/10.1016/j.aca.2023.341390
N2  - A new set-up for fully autonomous and high-throughput capillary electrophoresis (CE) analyses of dried blood spot (DBS) samples is presented. The DBS samples were prepared by collecting exactly 5 μL of capillary blood from a finger-prick onto a pre-punched DBS disc in a disposable plastic CE vial and by in-vial blood drying. The vials with the DBS samples were then loaded into a commercial CE instrument for a fully unmanned sample processing and analysis. A fused-silica capillary of the CE instrument was first used for the transfer of 100 μL of elution solvent to each vial, in-vial DBS elution, and in-vial eluate homogenization. The same capillary was also used for at-line injection, separation, and selective analysis of the resulting eluates. Novel CE sequences were tailor-programmed for consecutive processing and analyses of multiple DBSs, which facilitated a fully autonomous determination of uric acid with a throughput of 240 DBS samples per day (24 h). The presented analytical protocol (using 100 μm i. d./30 cm capillary; 30 mM 2-(N-morpholino)-ethanesulfonic acid, 30 mM l-histidine, and 30 μM cetyltrimethylammonium bromide background electrolyte solution; and UV detection at 292 nm) provided excellent precision at endogenous and spiked uric acid concentrations with RSD values of peak areas below 3.2%. Calibration curves were linear over the 33.3 − 1200 μM range (R2 better than 0.998), limits of detection and quantification in the original capillary blood were 10 and 33.3 μM, respectively, and were well below the uric acid clinical range (140–420 μM). The stability of uric acid in DBS samples stored at laboratory temperature for up to 2 months was also excellent demonstrating less than a 4.2% decrease in uric acid concentrations. The actual set-up might thus be highly attractive for clinical subjects and laboratories because it is minimally invasive and requires minimum intervention from laboratory staff.
ER  -

MORAVČÍK, Ondrej, Miloš DVOŘÁK a Pavel KUBÁŇ. Autonomous capillary electrophoresis processing and analysis of dried blood spots for high-throughput determination of uric acid. \textit{Analytica Chimica Acta}. Amsterdam: Elsevier, 2023, roč.~1267, August, s.~1-8. ISSN~0003-2670. Dostupné z: https://dx.doi.org/10.1016/j.aca.2023.341390.

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