| CHOCHOLOVÁ, Eva, Pavel ROUDNICKÝ, David POTĚŠIL, Dana FIALOVÁ, Karolína KRYŠTOFOVÁ, Eva DROZDOVÁ and Zbyněk ZDRÁHAL. Extraction Protocol for Parallel Analysis of Proteins and DNA from Ancient Teeth and Dental Calculus. Journal of Proteome Research. American Chemical Society, 2023, vol. 22, No 10, p. 3311-3319. ISSN 1535-3893. Available from: https://dx.doi.org/10.1021/acs.jproteome.3c00370. |
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@article{2313637,
author = {Chocholová, Eva and Roudnický, Pavel and Potěšil, David and Fialová, Dana and Kryštofová, Karolína and Drozdová, Eva and Zdráhal, Zbyněk},
article_number = {10},
doi = {http://dx.doi.org/10.1021/acs.jproteome.3c00370},
keywords = {bioarcheology; paleoproteomics; ancientDNA; ancientproteins; dental calculus},
language = {eng},
issn = {1535-3893},
journal = {Journal of Proteome Research},
title = {Extraction Protocol for Parallel Analysis of Proteins and DNA from Ancient Teeth and Dental Calculus},
url = {https://doi.org/10.1021/acs.jproteome.3c00370},
volume = {22},
year = {2023}
}
TY - JOUR
ID - 2313637
AU - Chocholová, Eva - Roudnický, Pavel - Potěšil, David - Fialová, Dana - Kryštofová, Karolína - Drozdová, Eva - Zdráhal, Zbyněk
PY - 2023
TI - Extraction Protocol for Parallel Analysis of Proteins and DNA from Ancient Teeth and Dental Calculus
JF - Journal of Proteome Research
VL - 22
IS - 10
SP - 3311-3319
EP - 3311-3319
PB - American Chemical Society
SN - 15353893
KW - bioarcheology
KW - paleoproteomics
KW - ancientDNA
KW - ancientproteins
KW - dental calculus
UR - https://doi.org/10.1021/acs.jproteome.3c00370
N2 - Dental calculus is becoming a crucial material in the study of past populations with increasing interest in its proteomic and genomic content. Here, we suggest further development of a protocol for analysis of ancient proteins and a combined approach for subsequent ancient DNA extraction. We tested the protocol on recent teeth, and the optimized protocol was applied to ancient tooth to limit the destruction of calculus as it is a precious and irreplaceable source of dietary, microbiological, and ecological information in the archeological context. Finally, the applicability of the protocol was demonstrated on samples of the ancient calculus.
ER -
CHOCHOLOVÁ, Eva, Pavel ROUDNICKÝ, David POTĚŠIL, Dana FIALOVÁ, Karolína KRYŠTOFOVÁ, Eva DROZDOVÁ and Zbyněk ZDRÁHAL. Extraction Protocol for Parallel Analysis of Proteins and DNA from Ancient Teeth and Dental Calculus. \textit{Journal of Proteome Research}. American Chemical Society, 2023, vol.~22, No~10, p.~3311-3319. ISSN~1535-3893. Available from: https://dx.doi.org/10.1021/acs.jproteome.3c00370.
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