Transcriptome Profile Analyses of Head Kidney in Roach (Rutilus
rutilus), Common Bream (Abramis brama) and Their Hybrids: Does
Infection by Monogenean Parasites in Freshwater Fish Reveal
Differences in Fish Vigour among Parental Species and Their
Hybrids?

J 2023

Transcriptome Profile Analyses of Head Kidney in Roach (Rutilus rutilus), Common Bream (Abramis brama) and Their Hybrids: Does Infection by Monogenean Parasites in Freshwater Fish Reveal Differences in Fish Vigour among Parental Species and Their Hybrids?

VETEŠNÍKOVÁ ŠIMKOVÁ, Andrea, Kristína KŘÍŽOVÁ, Kristýna VOŘÍŠKOVÁ, Lukáš VETEŠNÍK, Vojtěch BYSTRÝ et. al.

Základní údaje

Originální název

Transcriptome Profile Analyses of Head Kidney in Roach (Rutilus rutilus), Common Bream (Abramis brama) and Their Hybrids: Does Infection by Monogenean Parasites in Freshwater Fish Reveal Differences in Fish Vigour among Parental Species and Their Hybrids?

Autoři

VETEŠNÍKOVÁ ŠIMKOVÁ, Andrea (203 Česká republika, garant, domácí), Kristína KŘÍŽOVÁ (703 Slovensko, domácí), Kristýna VOŘÍŠKOVÁ (203 Česká republika, domácí), Lukáš VETEŠNÍK (203 Česká republika, domácí), Vojtěch BYSTRÝ (203 Česká republika, domácí) a Martin DEMKO (703 Slovensko, domácí)

Vydání

Biology, Basel, MDPI, 2023, 2079-7737

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.200 v roce 2022

Kód RIV

RIV/00216224:14310/23:00131742

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.3390/biology12091199

UT WoS

001076917100001

Klíčová slova anglicky

Monogenea; Paradiplozoon homoion; freshwater fish; hybridization; RNA seq; differential gene expression; hybrid heterosis; hybrid breakdown

Štítky

CF BIOIT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 8. 4. 2024 10:34, Mgr. Eva Dubská

Anotace

V originále

Interspecific hybrids of F1 generations have frequently been characterized by high vigour resulting from heterosis advantage. In contrast, post-F1 generations are expected to express hybrid breakdown, i.e., they suffer from low viability and survival, reproductive abnormalities or sterility and limited ecological performance. Resistance or susceptibility to parasites is one of the measures reflecting hybrid vigour. The present study aimed to analyse the experimental infection of the blood-feeding generalist parasite Paradiplozoon homoion (Monogenea) in two target fish species, Abramis brama and Rutilus rutilus, and their reciprocal F1 hybrids and backcross hybrids, and to reveal potential parasite-induced changes in their transcriptome profiles of head kidney. We hypothesized various effects of hybridization in terms of parasitism in F1 hybrids and backcross hybrids reflected by differential gene expression. The number of differentially expressed genes (DEGs) differed between fish lines with a lower number of DEGs in F1 hybrids and a higher number in backcross hybrids when compared to the parental lines, A. brama and R. rutilus. Backcross hybrids were more infected than F1 hybrids and parental lines. DEG analyses revealed the role of heme binding, erythrocyte differentiation and immunity-related processes in fish after infection by blood-feeding P. homoion. Using GO and KEGG analyses, we revealed the similarity in DEGs between two backcross generations of hybrids. This finding may indicate a potential consequence of hybrid breakdown in backcross generations. Gene expression in less parasitized F1 hybrids is in line of hybrid advantage.

Návaznosti

GA19-10088S, projekt VaV

Název: Genetická disrupce u mezidruhových hybridů kaprovitých ryb: její dopad na kondici-asociované znaky, imunitu a parazitární zátěž

Investor: Grantová agentura ČR, Genetická disrupce u mezidruhových hybridů kaprovitých ryb: její dopad na fitness-asociované znaky, metabolické náklady, imunitu a parazitární zátěž

90267, velká výzkumná infrastruktura

Název: NCMG III

Citovat

VETEŠNÍKOVÁ ŠIMKOVÁ, Andrea, Kristína KŘÍŽOVÁ, Kristýna VOŘÍŠKOVÁ, Lukáš VETEŠNÍK, Vojtěch BYSTRÝ a Martin DEMKO. Transcriptome Profile Analyses of Head Kidney in Roach (Rutilus rutilus), Common Bream (Abramis brama) and Their Hybrids: Does Infection by Monogenean Parasites in Freshwater Fish Reveal Differences in Fish Vigour among Parental Species and Their Hybrids? Biology. Basel: MDPI, 2023, roč. 12, č. 9, s. 1-29. ISSN 2079-7737. Dostupné z: https://dx.doi.org/10.3390/biology12091199.

@article{2314057,
   author = {Vetešníková Šimková, Andrea and Křížová, Kristína and Voříšková, Kristýna and Vetešník, Lukáš and Bystrý, Vojtěch and Demko, Martin},
   article_location = {Basel},
   article_number = {9},
   doi = {http://dx.doi.org/10.3390/biology12091199},
   keywords = {Monogenea; Paradiplozoon homoion; freshwater fish; hybridization; RNA seq; differential gene expression; hybrid heterosis; hybrid breakdown},
   language = {eng},
   issn = {2079-7737},
   journal = {Biology},
   title = {Transcriptome Profile Analyses of Head Kidney in Roach (Rutilus rutilus), Common Bream (Abramis brama) and Their Hybrids: Does Infection by Monogenean Parasites in Freshwater Fish Reveal Differences in Fish Vigour among Parental Species and Their Hybrids?},
   url = {https://doi.org/10.3390/biology12091199},
   volume = {12},
   year = {2023}
}

TY  - JOUR
ID  - 2314057
AU  - Vetešníková Šimková, Andrea - Křížová, Kristína - Voříšková, Kristýna - Vetešník, Lukáš - Bystrý, Vojtěch - Demko, Martin
PY  - 2023
TI  - Transcriptome Profile Analyses of Head Kidney in Roach (Rutilus rutilus), Common Bream (Abramis brama) and Their Hybrids: Does Infection by Monogenean Parasites in Freshwater Fish Reveal Differences in Fish Vigour among Parental Species and Their Hybrids?
JF  - Biology
VL  - 12
IS  - 9
SP  - 1-29
EP  - 1-29
PB  - MDPI
SN  - 20797737
KW  - Monogenea
KW  - Paradiplozoon homoion
KW  - freshwater fish
KW  - hybridization
KW  - RNA seq
KW  - differential gene expression
KW  - hybrid heterosis
KW  - hybrid breakdown
UR  - https://doi.org/10.3390/biology12091199
N2  - Interspecific hybrids of F1 generations have frequently been characterized by high vigour resulting from heterosis advantage. In contrast, post-F1 generations are expected to express hybrid breakdown, i.e., they suffer from low viability and survival, reproductive abnormalities or sterility and limited ecological performance. Resistance or susceptibility to parasites is one of the measures reflecting hybrid vigour. The present study aimed to analyse the experimental infection of the blood-feeding generalist parasite Paradiplozoon homoion (Monogenea) in two target fish species, Abramis brama and Rutilus rutilus, and their reciprocal F1 hybrids and backcross hybrids, and to reveal potential parasite-induced changes in their transcriptome profiles of head kidney. We hypothesized various effects of hybridization in terms of parasitism in F1 hybrids and backcross hybrids reflected by differential gene expression. The number of differentially expressed genes (DEGs) differed between fish lines with a lower number of DEGs in F1 hybrids and a higher number in backcross hybrids when compared to the parental lines, A. brama and R. rutilus. Backcross hybrids were more infected than F1 hybrids and parental lines. DEG analyses revealed the role of heme binding, erythrocyte differentiation and immunity-related processes in fish after infection by blood-feeding P. homoion. Using GO and KEGG analyses, we revealed the similarity in DEGs between two backcross generations of hybrids. This finding may indicate a potential consequence of hybrid breakdown in backcross generations. Gene expression in less parasitized F1 hybrids is in line of hybrid advantage.
ER  -

VETEŠNÍKOVÁ ŠIMKOVÁ, Andrea, Kristína KŘÍŽOVÁ, Kristýna VOŘÍŠKOVÁ, Lukáš VETEŠNÍK, Vojtěch BYSTRÝ a Martin DEMKO. Transcriptome Profile Analyses of Head Kidney in Roach (Rutilus rutilus), Common Bream (Abramis brama) and Their Hybrids: Does Infection by Monogenean Parasites in Freshwater Fish Reveal Differences in Fish Vigour among Parental Species and Their Hybrids? \textit{Biology}. Basel: MDPI, 2023, roč.~12, č.~9, s.~1-29. ISSN~2079-7737. Dostupné z: https://dx.doi.org/10.3390/biology12091199.

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