Natural Diet of European Green Lizards, Lacerta viridis
(Squamata: Lacertidae): A Comparison of Macroscopic and
Molecular Identification Methods

J 2023

Natural Diet of European Green Lizards, Lacerta viridis (Squamata: Lacertidae): A Comparison of Macroscopic and Molecular Identification Methods

PEKÁR, Stanislav, Domagoj GAJSKI, Tamara MIFKOVÁ, Radovan SMOLINSKÝ, Tomislav GOJAK et. al.

Základní údaje

Originální název

Natural Diet of European Green Lizards, Lacerta viridis (Squamata: Lacertidae): A Comparison of Macroscopic and Molecular Identification Methods

Autoři

PEKÁR, Stanislav (703 Slovensko, garant, domácí), Domagoj GAJSKI (191 Chorvatsko, domácí), Tamara MIFKOVÁ (203 Česká republika), Radovan SMOLINSKÝ (703 Slovensko, domácí), Tomislav GOJAK (191 Chorvatsko) a Martina MARTIŠOVÁ (203 Česká republika, domácí)

Vydání

Herpetologica, Herpetologist's League Inc. 2023, 0018-0831

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10613 Zoology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.400 v roce 2022

Kód RIV

RIV/00216224:14310/23:00134242

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1655/Herpetologica-D-23-00017

UT WoS

001086833400003

Klíčová slova anglicky

fecal analysis; metabarcoding; predator; prey; Reptile conservation

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 4. 3. 2024 10:30, Mgr. Marie Šípková, DiS.

Anotace

V originále

An analysis of the diets of reptiles is essential for understanding the role of reptiles in the ecosystem and the employment of successful conservation management plans. For this purpose, noninvasive and invasive methods to identify consumed prey have been used. Here, we investigated the diet of male and female European Green Lizards (Lacerta viridis) by sampling fecal pellets across 2 yr in the spring and late summer at a single site. We used the following two methods for identifying prey remnants from fecal samples: the classical macroscopic approach that requires competent expert knowledge and the molecular approach based on the dietary metabarcoding of nondegraded prey remnant DNA. According to both methods, lizards consumed mainly insects belonging to 13 orders, with Coleoptera as the dominant prey. The number of prey taxa was similar between the sexes, but the prey composition at the genus level was significantly different, with males capturing some coleopterans more than females. The diets also differed significantly between season. In the spring, lizards consumed many more prey types and many more coleopteran specimens than in late summer. The proportion of identified prey taxa was significantly different between the identification methods. From the total of identified prey, macroscopic identification yielded only about 50% of taxa, whereas molecular identification yielded more than 80% of taxa. Our results show that molecular identification can recover a much higher number of prey than the macroscopic method, yet not all prey. Thus, the integration of both methods best described the natural diet and complex trophic interactions of European Green Lizards.

Návaznosti

GA19-09323S, projekt VaV

Název: Mimetické komplexy a evoluce nepřesných mimetiků

Investor: Grantová agentura ČR, Mimetic complexes and the evolution of inaccurate mimics

Citovat

PEKÁR, Stanislav, Domagoj GAJSKI, Tamara MIFKOVÁ, Radovan SMOLINSKÝ, Tomislav GOJAK a Martina MARTIŠOVÁ. Natural Diet of European Green Lizards, Lacerta viridis (Squamata: Lacertidae): A Comparison of Macroscopic and Molecular Identification Methods. Herpetologica. Herpetologist's League Inc., 2023, roč. 79, č. 3, s. 135-143. ISSN 0018-0831. Dostupné z: https://dx.doi.org/10.1655/Herpetologica-D-23-00017.

@article{2317158,
   author = {Pekár, Stanislav and Gajski, Domagoj and Mifková, Tamara and Smolinský, Radovan and Gojak, Tomislav and Martišová, Martina},
   article_number = {3},
   doi = {http://dx.doi.org/10.1655/Herpetologica-D-23-00017},
   keywords = {fecal analysis; metabarcoding; predator; prey; Reptile conservation},
   language = {eng},
   issn = {0018-0831},
   journal = {Herpetologica},
   title = {Natural Diet of European Green Lizards, Lacerta viridis (Squamata: Lacertidae): A Comparison of Macroscopic and Molecular Identification Methods},
   url = {https://doi.org/10.1655/Herpetologica-D-23-00017},
   volume = {79},
   year = {2023}
}

TY  - JOUR
ID  - 2317158
AU  - Pekár, Stanislav - Gajski, Domagoj - Mifková, Tamara - Smolinský, Radovan - Gojak, Tomislav - Martišová, Martina
PY  - 2023
TI  - Natural Diet of European Green Lizards, Lacerta viridis (Squamata: Lacertidae): A Comparison of Macroscopic and Molecular Identification Methods
JF  - Herpetologica
VL  - 79
IS  - 3
SP  - 135-143
EP  - 135-143
PB  - Herpetologist's League Inc.
SN  - 00180831
KW  - fecal analysis
KW  - metabarcoding
KW  - predator
KW  - prey
KW  - Reptile conservation
UR  - https://doi.org/10.1655/Herpetologica-D-23-00017
N2  - An analysis of the diets of reptiles is essential for understanding the role of reptiles in the ecosystem and the employment of successful conservation management plans. For this purpose, noninvasive and invasive methods to identify consumed prey have been used. Here, we investigated the diet of male and female European Green Lizards (Lacerta viridis) by sampling fecal pellets across 2 yr in the spring and late summer at a single site. We used the following two methods for identifying prey remnants from fecal samples: the classical macroscopic approach that requires competent expert knowledge and the molecular approach based on the dietary metabarcoding of nondegraded prey remnant DNA. According to both methods, lizards consumed mainly insects belonging to 13 orders, with Coleoptera as the dominant prey. The number of prey taxa was similar between the sexes, but the prey composition at the genus level was significantly different, with males capturing some coleopterans more than females. The diets also differed significantly between season. In the spring, lizards consumed many more prey types and many more coleopteran specimens than in late summer. The proportion of identified prey taxa was significantly different between the identification methods. From the total of identified prey, macroscopic identification yielded only about 50% of taxa, whereas molecular identification yielded more than 80% of taxa. Our results show that molecular identification can recover a much higher number of prey than the macroscopic method, yet not all prey. Thus, the integration of both methods best described the natural diet and complex trophic interactions of European Green Lizards.
ER  -

PEKÁR, Stanislav, Domagoj GAJSKI, Tamara MIFKOVÁ, Radovan SMOLINSKÝ, Tomislav GOJAK a Martina MARTIŠOVÁ. Natural Diet of European Green Lizards, Lacerta viridis (Squamata: Lacertidae): A Comparison of Macroscopic and Molecular Identification Methods. \textit{Herpetologica}. Herpetologist's League Inc., 2023, roč.~79, č.~3, s.~135-143. ISSN~0018-0831. Dostupné z: https://dx.doi.org/10.1655/Herpetologica-D-23-00017.

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