Specific alterations of sphingolipid metabolism identified in
EpCAM-positive cells isolated from human colon tumors

J 2020

Specific alterations of sphingolipid metabolism identified in EpCAM-positive cells isolated from human colon tumors

PROCHÁZKOVÁ, Jiřina, Josef SLAVIK, Jan BOUCHAL, Monika LEVKOVA, Zlata HUSKOVA et. al.

Základní údaje

Originální název

Specific alterations of sphingolipid metabolism identified in EpCAM-positive cells isolated from human colon tumors

Autoři

Vydání

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, AMSTERDAM, ELSEVIER, 2020, 1388-1981

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.698

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.bbalip.2020.158742

UT WoS

000552710900012

Klíčová slova anglicky

Sphingolipid metabolism; Colon adenocarcinoma; Lactosylceramide; B4GALTs; EPCAM-positive cells

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 29. 9. 2023 10:10, Mgr. Jiřina Procházková, Ph.D.

Anotace

V originále

Metabolic reprogramming leading to alterations in lipid metabolism and lipid-mediated signaling may contribute to colorectal cancer (CRC) development and progression. We hypothesized that a detailed description of changes in sphingolipidome of specific cellular subpopulation residing in colon cancer tissue may help to discriminate between normal and transformed colon epithelial cells. Using HPLC-mass spectrometry, we analyzed the EpCAM-positive cells isolated from tumor and adjacent non-tumor tissues of colon cancer patients, aiming to identify potential lipid biomarkers specific for CRC. We then employed RT-qPCR-based methodology in order to analyze expression of SL metabolism-related genes in the isolated EpCAM-positive tumor cells and/or in unseparated colon tumor tissues. We observed significant changes in sphingolipid (SL) species in the EpCAM-positive tumor cells, with the accumulation of lactosylceramide (LacCer) being the most prominent. B4GALT5 and B4GALT6 genes were identified as two potential gene candidates contributing to LacCer accumulation. We further identified additional genes of SL and fatty acid metabolism (e.g. SPHK1, GBA2, NEU3, GLA, FASN, PLA2G10), which were significantly altered in colon tumor tissue. The present results indicate that the EpCAM-positive cells are a major contributor to LacCer accumulation in CRC tissue, and may help to identify novel CRC-specific lipid/gene biomarkers.

Citovat

PROCHÁZKOVÁ, Jiřina, Josef SLAVIK, Jan BOUCHAL, Monika LEVKOVA, Zlata HUSKOVA, Jiri EHRMANN, Petra OVESNÁ, Zdenek KOLAR, Pavel SKALICKY, Nicol STRAKOVA, Ondrej ZAPLETAL, Alois KOZUBÍK, Jiřina HOFMANOVÁ, Jan VONDRÁČEK a Miroslav MACHALA. Specific alterations of sphingolipid metabolism identified in EpCAM-positive cells isolated from human colon tumors. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS. AMSTERDAM: ELSEVIER, 2020, roč. 1865, č. 9, s. 1-11. ISSN 1388-1981. Dostupné z: https://dx.doi.org/10.1016/j.bbalip.2020.158742.

@article{2317997,
   author = {Procházková, Jiřina and Slavik, Josef and Bouchal, Jan and Levkova, Monika and Huskova, Zlata and Ehrmann, Jiri and Ovesná, Petra and Kolar, Zdenek and Skalicky, Pavel and Strakova, Nicol and Zapletal, Ondrej and Kozubík, Alois and Hofmanová, Jiřina and Vondráček, Jan and Machala, Miroslav},
   article_location = {AMSTERDAM},
   article_number = {9},
   doi = {http://dx.doi.org/10.1016/j.bbalip.2020.158742},
   keywords = {Sphingolipid metabolism; Colon adenocarcinoma; Lactosylceramide; B4GALTs; EPCAM-positive cells},
   language = {eng},
   issn = {1388-1981},
   journal = {BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS},
   title = {Specific alterations of sphingolipid metabolism identified in EpCAM-positive cells isolated from human colon tumors},
   url = {https://www.sciencedirect.com/science/article/pii/S1388198120301347?via%3Dihub},
   volume = {1865},
   year = {2020}
}

TY  - JOUR
ID  - 2317997
AU  - Procházková, Jiřina - Slavik, Josef - Bouchal, Jan - Levkova, Monika - Huskova, Zlata - Ehrmann, Jiri - Ovesná, Petra - Kolar, Zdenek - Skalicky, Pavel - Strakova, Nicol - Zapletal, Ondrej - Kozubík, Alois - Hofmanová, Jiřina - Vondráček, Jan - Machala, Miroslav
PY  - 2020
TI  - Specific alterations of sphingolipid metabolism identified in EpCAM-positive cells isolated from human colon tumors
JF  - BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
VL  - 1865
IS  - 9
SP  - 1-11
EP  - 1-11
PB  - ELSEVIER
SN  - 13881981
KW  - Sphingolipid metabolism
KW  - Colon adenocarcinoma
KW  - Lactosylceramide
KW  - B4GALTs
KW  - EPCAM-positive cells
UR  - https://www.sciencedirect.com/science/article/pii/S1388198120301347?via%3Dihub
N2  - Metabolic reprogramming leading to alterations in lipid metabolism and lipid-mediated signaling may contribute to colorectal cancer (CRC) development and progression. We hypothesized that a detailed description of changes in sphingolipidome of specific cellular subpopulation residing in colon cancer tissue may help to discriminate between normal and transformed colon epithelial cells. Using HPLC-mass spectrometry, we analyzed the EpCAM-positive cells isolated from tumor and adjacent non-tumor tissues of colon cancer patients, aiming to identify potential lipid biomarkers specific for CRC. We then employed RT-qPCR-based methodology in order to analyze expression of SL metabolism-related genes in the isolated EpCAM-positive tumor cells and/or in unseparated colon tumor tissues. We observed significant changes in sphingolipid (SL) species in the EpCAM-positive tumor cells, with the accumulation of lactosylceramide (LacCer) being the most prominent. B4GALT5 and B4GALT6 genes were identified as two potential gene candidates contributing to LacCer accumulation. We further identified additional genes of SL and fatty acid metabolism (e.g. SPHK1, GBA2, NEU3, GLA, FASN, PLA2G10), which were significantly altered in colon tumor tissue. The present results indicate that the EpCAM-positive cells are a major contributor to LacCer accumulation in CRC tissue, and may help to identify novel CRC-specific lipid/gene biomarkers.
ER  -

PROCHÁZKOVÁ, Jiřina, Josef SLAVIK, Jan BOUCHAL, Monika LEVKOVA, Zlata HUSKOVA, Jiri EHRMANN, Petra OVESNÁ, Zdenek KOLAR, Pavel SKALICKY, Nicol STRAKOVA, Ondrej ZAPLETAL, Alois KOZUBÍK, Jiřina HOFMANOVÁ, Jan VONDRÁČEK a Miroslav MACHALA. Specific alterations of sphingolipid metabolism identified in EpCAM-positive cells isolated from human colon tumors. \textit{BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS}. AMSTERDAM: ELSEVIER, 2020, roč.~1865, č.~9, s.~1-11. ISSN~1388-1981. Dostupné z: https://dx.doi.org/10.1016/j.bbalip.2020.158742.

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