Dispersal and invasive stages of Urospora eugregarines
(Apicomplexa) from brown bodies of a polychaete host

J 2023

Dispersal and invasive stages of Urospora eugregarines (Apicomplexa) from brown bodies of a polychaete host

BARDŮNEK VALIGUROVÁ, Andrea, Andrei DIAKIN, Mária SEIFERTOVÁ, Naděžda VAŠKOVICOVÁ, Magdaléna KOVÁČIKOVÁ et. al.

Základní údaje

Originální název

Dispersal and invasive stages of Urospora eugregarines (Apicomplexa) from brown bodies of a polychaete host

Autoři

BARDŮNEK VALIGUROVÁ, Andrea (703 Slovensko, garant, domácí), Andrei DIAKIN (643 Rusko, domácí), Mária SEIFERTOVÁ (703 Slovensko, domácí), Naděžda VAŠKOVICOVÁ (203 Česká republika, domácí), Magdaléna KOVÁČIKOVÁ (703 Slovensko, domácí) a Gita G. PASKEROVA (643 Rusko)

Vydání

Journal of Invertebrate Pathology, Elsevier Inc. 2023, 0022-2011

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10613 Zoology

Stát vydavatele

Kanada

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL URL

Impakt faktor

Impact factor: 3.400 v roce 2022

Kód RIV

RIV/00216224:14310/23:00134245

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.jip.2023.107997

UT WoS

001089301500001

Klíčová slova anglicky

Invertebrate immunity; gametocyst; oocyst; sporozoite;18S and 28S rDNAs; Urosporidae

Štítky

14110517, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 8. 3. 2024 13:19, Mgr. Marie Šípková, DiS.

Anotace

V originále

Urosporid eugregarines (Apicomplexa: Urosporidae) are unicellular eukaryotic parasites inhabiting the coelom or the intestine of marine invertebrates such as annelids, molluscs, nemerteans, and echinoderms. Despite the availability of published morphological and phylogenetical analyses of coelomic gregarines, their long-term survival in the host body cavity and dispersal routes into the marine environment remain unclear. Here, we focus on Urospora gametocysts and oocysts with sporozoites, which were found viable inside the so-called brown bodies floating in the body cavity of the polychaete Travisia forbesii. Brown bodies form as a result of host defence where coelomocytes encapsulate dead host cells and foreign objects including potential pathogens. We hypothesise the long-term persistence of Urospora eugregarines in brown bodies through evasion of the host immunity and outline possible pathways for their egress into the marine environment, applicable as dispersal routes for other parasites as well. Unique features revealed by detailed ultrastructural analysis of detected eugregarine stages include asynchronous sporogony, a massive sporozoite secretion apparatus, as well as the presence of free (possibly autoinfective) sporozoites within the gametocyst. The assignment to the genus Urospora and the complete identity with U. ovalis and U. travisiae were confirmed by analysing 18S rDNA sequences obtained from isolated gametocysts. The 18S rDNA phylogeny confirmed the affiliation of Urosporidae to Lecudinoidea and the grouping of all Urospora sequences with Difficilina from nemerteans and environmental sequences from the Artic region. We also enriched the Apicomplexa set by partial 28S rDNA sequences of two Urospora species enabling more complex phylogenetic analyses prospectively.

Návaznosti

GBP505/12/G112, projekt VaV

Název: ECIP - Evropské centrum ichtyoparazitologie

Investor: Grantová agentura ČR, ECIP - Evropské centrum ichtyoparazitologie

Citovat

BARDŮNEK VALIGUROVÁ, Andrea, Andrei DIAKIN, Mária SEIFERTOVÁ, Naděžda VAŠKOVICOVÁ, Magdaléna KOVÁČIKOVÁ a Gita G. PASKEROVA. Dispersal and invasive stages of Urospora eugregarines (Apicomplexa) from brown bodies of a polychaete host. Journal of Invertebrate Pathology. Elsevier Inc., 2023, roč. 201, November 2023, s. 1-17. ISSN 0022-2011. Dostupné z: https://dx.doi.org/10.1016/j.jip.2023.107997.

@article{2319078,
   author = {Bardůnek Valigurová, Andrea and Diakin, Andrei and Seifertová, Mária and Vaškovicová, Naděžda and Kováčiková, Magdaléna and Paskerova, Gita G.},
   article_number = {November 2023},
   doi = {http://dx.doi.org/10.1016/j.jip.2023.107997},
   keywords = {Invertebrate immunity; gametocyst; oocyst; sporozoite;18S and 28S rDNAs; Urosporidae},
   language = {eng},
   issn = {0022-2011},
   journal = {Journal of Invertebrate Pathology},
   title = {Dispersal and invasive stages of Urospora eugregarines (Apicomplexa) from brown bodies of a polychaete host},
   url = {https://www.sciencedirect.com/science/article/pii/S0022201123001143},
   volume = {201},
   year = {2023}
}

TY  - JOUR
ID  - 2319078
AU  - Bardůnek Valigurová, Andrea - Diakin, Andrei - Seifertová, Mária - Vaškovicová, Naděžda - Kováčiková, Magdaléna - Paskerova, Gita G.
PY  - 2023
TI  - Dispersal and invasive stages of Urospora eugregarines (Apicomplexa) from brown bodies of a polychaete host
JF  - Journal of Invertebrate Pathology
VL  - 201
IS  - November 2023
SP  - 1-17
EP  - 1-17
PB  - Elsevier Inc.
SN  - 00222011
KW  - Invertebrate immunity
KW  - gametocyst
KW  - oocyst
KW  - sporozoite;18S and 28S rDNAs
KW  - Urosporidae
UR  - https://www.sciencedirect.com/science/article/pii/S0022201123001143
N2  - Urosporid eugregarines (Apicomplexa: Urosporidae) are unicellular eukaryotic parasites inhabiting the coelom or the intestine of marine invertebrates such as annelids, molluscs, nemerteans, and echinoderms. Despite the availability of published morphological and phylogenetical analyses of coelomic gregarines, their long-term survival in the host body cavity and dispersal routes into the marine environment remain unclear. Here, we focus on Urospora gametocysts and oocysts with sporozoites, which were found viable inside the so-called brown bodies floating in the body cavity of the polychaete Travisia forbesii. Brown bodies form as a result of host defence where coelomocytes encapsulate dead host cells and foreign objects including potential pathogens. We hypothesise the long-term persistence of Urospora eugregarines in brown bodies through evasion of the host immunity and outline possible pathways for their egress into the marine environment, applicable as dispersal routes for other parasites as well. Unique features revealed by detailed ultrastructural analysis of detected eugregarine stages include asynchronous sporogony, a massive sporozoite secretion apparatus, as well as the presence of free (possibly autoinfective) sporozoites within the gametocyst. The assignment to the genus Urospora and the complete identity with U. ovalis and U. travisiae were confirmed by analysing 18S rDNA sequences obtained from isolated gametocysts. The 18S rDNA phylogeny confirmed the affiliation of Urosporidae to Lecudinoidea and the grouping of all Urospora sequences with Difficilina from nemerteans and environmental sequences from the Artic region. We also enriched the Apicomplexa set by partial 28S rDNA sequences of two Urospora species enabling more complex phylogenetic analyses prospectively.
ER  -

BARDŮNEK VALIGUROVÁ, Andrea, Andrei DIAKIN, Mária SEIFERTOVÁ, Naděžda VAŠKOVICOVÁ, Magdaléna KOVÁČIKOVÁ a Gita G. PASKEROVA. Dispersal and invasive stages of Urospora eugregarines (Apicomplexa) from brown bodies of a polychaete host. \textit{Journal of Invertebrate Pathology}. Elsevier Inc., 2023, roč.~201, November 2023, s.~1-17. ISSN~0022-2011. Dostupné z: https://dx.doi.org/10.1016/j.jip.2023.107997.

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