TOOLS TO STUDY DOSAGE COMPENSATION AND PARENTAL IMPRINTING IN
SILENE LATIFOLIA

a 2023

TOOLS TO STUDY DOSAGE COMPENSATION AND PARENTAL IMPRINTING IN SILENE LATIFOLIA

NOVOTNÁ, Pavla, Václav BAČOVSKÝ, Aline MUYLE a Andreas HOUBEN

Základní údaje

Originální název

TOOLS TO STUDY DOSAGE COMPENSATION AND PARENTAL IMPRINTING IN SILENE LATIFOLIA

Autoři

NOVOTNÁ, Pavla (203 Česká republika, domácí), Václav BAČOVSKÝ (203 Česká republika), Aline MUYLE (250 Francie) a Andreas HOUBEN (276 Německo)

Vydání

Methods in Plant Sciences, 2023

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Kód RIV

RIV/00216224:14310/23:00131845

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

X chromosome inactivation; Y degeneration; imprinting; replication pattern

Změněno: 20. 10. 2023 15:19, Ing. Pavla Novotná

Anotace

V originále

Dosage compensatory mechanism (DC) refers to the equalization of X-linked gene products between males and females. In old sex chromosomes systems, DC usually affects the entire X chromosomes with only few genes capable of inactivation escape. Nevertheless, in species with evolutionary young sex chromosomes, DC exhibits rather local or incomplete X chromosome inactivation, proceeding gene-by-gene or by whole locus deregulation. Despite the evidence given for the main animal species, the mechanisms through X chromosome inactivation evolves remain still enigmatic. To study early stages of DC and expression balance between sexes, we use combination of cytogenetic and NGS techniques in a species with young sex chromosome system, Silene latifolia. Using X chromosome specific marker and EdU that is incorporated into newly synthesized DNA strands, we distinguished paternal and maternal Xs. We show that parental X chromosome in females is late replicated, supported by previous evidence of transcriptome profiling and localization of active histone marks. Interestingly, only a chromosomal arm carrying PAR shows replication delay that suggests locus specific incomplete DC. Further, we ask weather polyploidy may disturb dosage compensation pattern in this model plant. To answer this question, we developed a set of tetraploid and triploid individuals with different sex chromosome constitution. This will help us to understand how and what (epi)genetic processes take a role in DC evolution, and help us to shed more light on the most fundamental question of sex chromosome biology.

Návaznosti

MUNI/A/1200/2022, interní kód MU

Název: Specifický výzkum s využitím přístupů funkční genomiky a proteomiky

Investor: Masarykova univerzita, Specifický výzkum s využitím přístupů funkční genomiky a proteomiky

Citovat

NOVOTNÁ, Pavla, Václav BAČOVSKÝ, Aline MUYLE a Andreas HOUBEN. TOOLS TO STUDY DOSAGE COMPENSATION AND PARENTAL IMPRINTING IN SILENE LATIFOLIA. In Methods in Plant Sciences. 2023.

@proceedings{2321117,
   author = {Novotná, Pavla and Bačovský, Václav and Muyle, Aline and Houben, Andreas},
   booktitle = {Methods in Plant Sciences},
   keywords = {X chromosome inactivation; Y degeneration; imprinting; replication pattern},
   language = {eng},
   title = {TOOLS TO STUDY DOSAGE COMPENSATION AND PARENTAL IMPRINTING IN SILENE LATIFOLIA},
   url = {https://csebr.cz/vse-cs/methods-in-plant-science-2023/},
   year = {2023}
}

TY  - CONF
ID  - 2321117
AU  - Novotná, Pavla - Bačovský, Václav - Muyle, Aline - Houben, Andreas
PY  - 2023
TI  - TOOLS TO STUDY DOSAGE COMPENSATION AND PARENTAL IMPRINTING IN SILENE LATIFOLIA
KW  - X chromosome inactivation
KW  - Y degeneration
KW  - imprinting
KW  - replication pattern
UR  - https://csebr.cz/vse-cs/methods-in-plant-science-2023/
N2  - Dosage compensatory mechanism (DC) refers to the equalization of X-linked gene products between males and females. In old sex chromosomes systems, DC usually affects the entire X chromosomes with only few genes capable of inactivation escape. Nevertheless, in species with evolutionary young sex chromosomes, DC exhibits rather local or incomplete X chromosome inactivation, proceeding gene-by-gene or by whole locus deregulation. Despite the evidence given for the main animal species, the mechanisms through X chromosome inactivation evolves remain still enigmatic. To study early stages of DC and expression balance between sexes, we use combination of cytogenetic and NGS techniques in a species with young sex chromosome system, Silene latifolia. Using X chromosome specific marker and EdU that is incorporated into newly synthesized DNA strands, we distinguished paternal and maternal Xs. We show that parental X chromosome in females is late replicated, supported by previous evidence of transcriptome profiling and localization of active histone marks. Interestingly, only a chromosomal arm carrying PAR shows replication delay that suggests locus specific incomplete DC. Further, we ask weather polyploidy may disturb dosage compensation pattern in this model plant. To answer this question, we developed a set of tetraploid and triploid individuals with different sex chromosome constitution. This will help us to understand how and what (epi)genetic processes take a role in DC evolution, and help us to shed more light on the most fundamental question of sex chromosome biology.
ER  -

NOVOTNÁ, Pavla, Václav BAČOVSKÝ, Aline MUYLE a Andreas HOUBEN. TOOLS TO STUDY DOSAGE COMPENSATION AND PARENTAL IMPRINTING IN SILENE LATIFOLIA. In \textit{Methods in Plant Sciences}. 2023.

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