Bio-AFM exploits enhanced response of human gingival
fibroblasts on TiO2 nanotubular substrates with thin TiO2
coatings

J 2023

Bio-AFM exploits enhanced response of human gingival fibroblasts on TiO2 nanotubular substrates with thin TiO2 coatings

BAISHYA, Kaushik, Kateřina VRCHOVECKÁ, Mahnaz ALIJANI, Jhonatan RODRIGUEZ-PEREIRA, Sitaramanjaneya Mouli THALLURI et. al.

Základní údaje

Originální název

Bio-AFM exploits enhanced response of human gingival fibroblasts on TiO2 nanotubular substrates with thin TiO2 coatings

Autoři

BAISHYA, Kaushik, Kateřina VRCHOVECKÁ (203 Česká republika, domácí), Mahnaz ALIJANI, Jhonatan RODRIGUEZ-PEREIRA, Sitaramanjaneya Mouli THALLURI, Monika PÁVKOVÁ GOLDBERGOVÁ (203 Česká republika, domácí), Jan PŘIBYL (203 Česká republika, domácí) a Jan M. MACAK (203 Česká republika, garant)

Vydání

Applied Surface Science Advances, AMSTERDAM, ELSEVIER, 2023, 2666-5239

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.200 v roce 2022

Kód RIV

RIV/00216224:14110/23:00131855

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1016/j.apsadv.2023.100459

UT WoS

001088513800001

Klíčová slova anglicky

TiO2; Nanotubeh; GFs; Ti foils; Atomic layer deposition; Bio-AFM

Štítky

14110518, CF NANO, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 18. 10. 2024 09:19, Mgr. Adéla Pešková

Anotace

V originále

The present work studies anodic TiO2 nanotube (TNT) layers and their surface modifications for enhancing the cell behavior of human gingival fibroblast cells (hGFs) with the contribution of bio-AFM (Atomic Force Microscopy) method. TNT layers, prepared via electrochemical anodization of Ti, with an average tube diameter of 15, 30, and 100 nm, were used as primary substrates for the study. Flat Ti foils were used as reference substrates. Part of the substrates was coated by ultrathin TiO2 coatings (≈ 0.3 nm thin) using Atomic Layer Deposition (ALD). The cell growth and adhesion of hGFs on the TNT layers and Ti foils were compared between ALD coated and uncoated ones. The supplemental coatings altered the surface chemistry of the TNT layers, particularly shading the fluorine and carbon impurities within anodic TiO2, while preserving the original structure and morphology. The presented approach of very mild surface modification remarkably effects the material's biocompatibility and possess great prospect as implant materials. For the first time, the TNT/cell interface was investigated using bio-AFM in terms of Young's modulus, stiffness, cell adhesive force and roughness. Improved biocompatibility was studied in terms of increased cell viability, density, cell cytoskeleton orientation and overall stiffness of the hGFs.

Návaznosti

EF18_046/0015974, projekt VaV

Název: Modernizace České infrastruktury pro integrativní strukturní biologii

LM2018127, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

90103, velká výzkumná infrastruktura

Název: CEMNAT II

90110, velká výzkumná infrastruktura

Název: CzechNanoLab

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

BAISHYA, Kaushik, Kateřina VRCHOVECKÁ, Mahnaz ALIJANI, Jhonatan RODRIGUEZ-PEREIRA, Sitaramanjaneya Mouli THALLURI, Monika PÁVKOVÁ GOLDBERGOVÁ, Jan PŘIBYL a Jan M. MACAK. Bio-AFM exploits enhanced response of human gingival fibroblasts on TiO2 nanotubular substrates with thin TiO2 coatings. Applied Surface Science Advances. AMSTERDAM: ELSEVIER, 2023, roč. 18, December 2023, s. 1-12. ISSN 2666-5239. Dostupné z: https://dx.doi.org/10.1016/j.apsadv.2023.100459.

@article{2322597,
   author = {Baishya, Kaushik and Vrchovecká, Kateřina and Alijani, Mahnaz and RodriguezandPereira, Jhonatan and Thalluri, Sitaramanjaneya Mouli and Pávková Goldbergová, Monika and Přibyl, Jan and Macak, Jan M.},
   article_location = {AMSTERDAM},
   article_number = {December 2023},
   doi = {http://dx.doi.org/10.1016/j.apsadv.2023.100459},
   keywords = {TiO2; Nanotubeh; GFs; Ti foils; Atomic layer deposition; Bio-AFM},
   language = {eng},
   issn = {2666-5239},
   journal = {Applied Surface Science Advances},
   title = {Bio-AFM exploits enhanced response of human gingival fibroblasts on TiO2 nanotubular substrates with thin TiO2 coatings},
   url = {https://www.sciencedirect.com/science/article/pii/S2666523923000934},
   volume = {18},
   year = {2023}
}

TY  - JOUR
ID  - 2322597
AU  - Baishya, Kaushik - Vrchovecká, Kateřina - Alijani, Mahnaz - Rodriguez-Pereira, Jhonatan - Thalluri, Sitaramanjaneya Mouli - Pávková Goldbergová, Monika - Přibyl, Jan - Macak, Jan M.
PY  - 2023
TI  - Bio-AFM exploits enhanced response of human gingival fibroblasts on TiO2 nanotubular substrates with thin TiO2 coatings
JF  - Applied Surface Science Advances
VL  - 18
IS  - December 2023
SP  - 1-12
EP  - 1-12
PB  - ELSEVIER
SN  - 26665239
KW  - TiO2
KW  - Nanotubeh
KW  - GFs
KW  - Ti foils
KW  - Atomic layer deposition
KW  - Bio-AFM
UR  - https://www.sciencedirect.com/science/article/pii/S2666523923000934
N2  - The present work studies anodic TiO2 nanotube (TNT) layers and their surface modifications for enhancing the cell behavior of human gingival fibroblast cells (hGFs) with the contribution of bio-AFM (Atomic Force Microscopy) method. TNT layers, prepared via electrochemical anodization of Ti, with an average tube diameter of 15, 30, and 100 nm, were used as primary substrates for the study. Flat Ti foils were used as reference substrates. Part of the substrates was coated by ultrathin TiO2 coatings (≈ 0.3 nm thin) using Atomic Layer Deposition (ALD). The cell growth and adhesion of hGFs on the TNT layers and Ti foils were compared between ALD coated and uncoated ones. The supplemental coatings altered the surface chemistry of the TNT layers, particularly shading the fluorine and carbon impurities within anodic TiO2, while preserving the original structure and morphology. The presented approach of very mild surface modification remarkably effects the material's biocompatibility and possess great prospect as implant materials. For the first time, the TNT/cell interface was investigated using bio-AFM in terms of Young's modulus, stiffness, cell adhesive force and roughness. Improved biocompatibility was studied in terms of increased cell viability, density, cell cytoskeleton orientation and overall stiffness of the hGFs.
ER  -

BAISHYA, Kaushik, Kateřina VRCHOVECKÁ, Mahnaz ALIJANI, Jhonatan RODRIGUEZ-PEREIRA, Sitaramanjaneya Mouli THALLURI, Monika PÁVKOVÁ GOLDBERGOVÁ, Jan PŘIBYL a Jan M. MACAK. Bio-AFM exploits enhanced response of human gingival fibroblasts on TiO2 nanotubular substrates with thin TiO2 coatings. \textit{Applied Surface Science Advances}. AMSTERDAM: ELSEVIER, 2023, roč.~18, December 2023, s.~1-12. ISSN~2666-5239. Dostupné z: https://dx.doi.org/10.1016/j.apsadv.2023.100459.

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