Bio-AFM exploits enhanced response of human gingival
fibroblasts on TiO2 nanotubular substrates with thin TiO2
coatings

J 2023

Bio-AFM exploits enhanced response of human gingival fibroblasts on TiO2 nanotubular substrates with thin TiO2 coatings

BAISHYA, Kaushik, Kateřina VRCHOVECKÁ, Mahnaz ALIJANI, Jhonatan RODRIGUEZ-PEREIRA, Sitaramanjaneya Mouli THALLURI et. al.

Basic information

Original name

Bio-AFM exploits enhanced response of human gingival fibroblasts on TiO2 nanotubular substrates with thin TiO2 coatings

Authors

BAISHYA, Kaushik, Kateřina VRCHOVECKÁ (203 Czech Republic, belonging to the institution), Mahnaz ALIJANI, Jhonatan RODRIGUEZ-PEREIRA, Sitaramanjaneya Mouli THALLURI, Monika PÁVKOVÁ GOLDBERGOVÁ (203 Czech Republic, belonging to the institution), Jan PŘIBYL (203 Czech Republic, belonging to the institution) and Jan M. MACAK (203 Czech Republic, guarantor)

Edition

Applied Surface Science Advances, AMSTERDAM, ELSEVIER, 2023, 2666-5239

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 6.200 in 2022

RIV identification code

RIV/00216224:14110/23:00131855

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.apsadv.2023.100459

UT WoS

001088513800001

Keywords in English

TiO2; Nanotubeh; GFs; Ti foils; Atomic layer deposition; Bio-AFM

Abstract

V originále

The present work studies anodic TiO2 nanotube (TNT) layers and their surface modifications for enhancing the cell behavior of human gingival fibroblast cells (hGFs) with the contribution of bio-AFM (Atomic Force Microscopy) method. TNT layers, prepared via electrochemical anodization of Ti, with an average tube diameter of 15, 30, and 100 nm, were used as primary substrates for the study. Flat Ti foils were used as reference substrates. Part of the substrates was coated by ultrathin TiO2 coatings (≈ 0.3 nm thin) using Atomic Layer Deposition (ALD). The cell growth and adhesion of hGFs on the TNT layers and Ti foils were compared between ALD coated and uncoated ones. The supplemental coatings altered the surface chemistry of the TNT layers, particularly shading the fluorine and carbon impurities within anodic TiO2, while preserving the original structure and morphology. The presented approach of very mild surface modification remarkably effects the material's biocompatibility and possess great prospect as implant materials. For the first time, the TNT/cell interface was investigated using bio-AFM in terms of Young's modulus, stiffness, cell adhesive force and roughness. Improved biocompatibility was studied in terms of increased cell viability, density, cell cytoskeleton orientation and overall stiffness of the hGFs.

Links

EF18_046/0015974, research and development project

Name: Modernizace České infrastruktury pro integrativní strukturní biologii

LM2018127, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

90103, large research infrastructures

Name: CEMNAT II

90110, large research infrastructures

Name: CzechNanoLab

90127, large research infrastructures

Name: CIISB II

Citovat

BAISHYA, Kaushik, Kateřina VRCHOVECKÁ, Mahnaz ALIJANI, Jhonatan RODRIGUEZ-PEREIRA, Sitaramanjaneya Mouli THALLURI, Monika PÁVKOVÁ GOLDBERGOVÁ, Jan PŘIBYL and Jan M. MACAK. Bio-AFM exploits enhanced response of human gingival fibroblasts on TiO2 nanotubular substrates with thin TiO2 coatings. Applied Surface Science Advances. AMSTERDAM: ELSEVIER, 2023, vol. 18, December 2023, p. 1-12. ISSN 2666-5239. Available from: https://dx.doi.org/10.1016/j.apsadv.2023.100459.

@article{2322597,
   author = {Baishya, Kaushik and Vrchovecká, Kateřina and Alijani, Mahnaz and RodriguezandPereira, Jhonatan and Thalluri, Sitaramanjaneya Mouli and Pávková Goldbergová, Monika and Přibyl, Jan and Macak, Jan M.},
   article_location = {AMSTERDAM},
   article_number = {December 2023},
   doi = {http://dx.doi.org/10.1016/j.apsadv.2023.100459},
   keywords = {TiO2; Nanotubeh; GFs; Ti foils; Atomic layer deposition; Bio-AFM},
   language = {eng},
   issn = {2666-5239},
   journal = {Applied Surface Science Advances},
   title = {Bio-AFM exploits enhanced response of human gingival fibroblasts on TiO2 nanotubular substrates with thin TiO2 coatings},
   url = {https://www.sciencedirect.com/science/article/pii/S2666523923000934},
   volume = {18},
   year = {2023}
}

TY  - JOUR
ID  - 2322597
AU  - Baishya, Kaushik - Vrchovecká, Kateřina - Alijani, Mahnaz - Rodriguez-Pereira, Jhonatan - Thalluri, Sitaramanjaneya Mouli - Pávková Goldbergová, Monika - Přibyl, Jan - Macak, Jan M.
PY  - 2023
TI  - Bio-AFM exploits enhanced response of human gingival fibroblasts on TiO2 nanotubular substrates with thin TiO2 coatings
JF  - Applied Surface Science Advances
VL  - 18
IS  - December 2023
SP  - 1-12
EP  - 1-12
PB  - ELSEVIER
SN  - 26665239
KW  - TiO2
KW  - Nanotubeh
KW  - GFs
KW  - Ti foils
KW  - Atomic layer deposition
KW  - Bio-AFM
UR  - https://www.sciencedirect.com/science/article/pii/S2666523923000934
N2  - The present work studies anodic TiO2 nanotube (TNT) layers and their surface modifications for enhancing the cell behavior of human gingival fibroblast cells (hGFs) with the contribution of bio-AFM (Atomic Force Microscopy) method. TNT layers, prepared via electrochemical anodization of Ti, with an average tube diameter of 15, 30, and 100 nm, were used as primary substrates for the study. Flat Ti foils were used as reference substrates. Part of the substrates was coated by ultrathin TiO2 coatings (≈ 0.3 nm thin) using Atomic Layer Deposition (ALD). The cell growth and adhesion of hGFs on the TNT layers and Ti foils were compared between ALD coated and uncoated ones. The supplemental coatings altered the surface chemistry of the TNT layers, particularly shading the fluorine and carbon impurities within anodic TiO2, while preserving the original structure and morphology. The presented approach of very mild surface modification remarkably effects the material's biocompatibility and possess great prospect as implant materials. For the first time, the TNT/cell interface was investigated using bio-AFM in terms of Young's modulus, stiffness, cell adhesive force and roughness. Improved biocompatibility was studied in terms of increased cell viability, density, cell cytoskeleton orientation and overall stiffness of the hGFs.
ER  -

BAISHYA, Kaushik, Kateřina VRCHOVECKÁ, Mahnaz ALIJANI, Jhonatan RODRIGUEZ-PEREIRA, Sitaramanjaneya Mouli THALLURI, Monika PÁVKOVÁ GOLDBERGOVÁ, Jan PŘIBYL and Jan M. MACAK. Bio-AFM exploits enhanced response of human gingival fibroblasts on TiO2 nanotubular substrates with thin TiO2 coatings. \textit{Applied Surface Science Advances}. AMSTERDAM: ELSEVIER, 2023, vol.~18, December 2023, p.~1-12. ISSN~2666-5239. Available from: https://dx.doi.org/10.1016/j.apsadv.2023.100459.

Detailed Information on Publication Record