Tau tubulin kinase 1 and 2 regulate ciliogenesis and human
pluripotent stem cells-derived neural rosettes

J 2023

Tau tubulin kinase 1 and 2 regulate ciliogenesis and human pluripotent stem cells-derived neural rosettes

BINÓ, Lucia a Lukáš ČAJÁNEK

Základní údaje

Originální název

Tau tubulin kinase 1 and 2 regulate ciliogenesis and human pluripotent stem cells-derived neural rosettes

Autoři

BINÓ, Lucia (703 Slovensko, garant, domácí) a Lukáš ČAJÁNEK (203 Česká republika, domácí)

Vydání

Scientific Reports, Berlin, Nature Research, 2023, 2045-2322

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.600 v roce 2022

Kód RIV

RIV/00216224:14110/23:00131886

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1038/s41598-023-39887-9

UT WoS

001045574100047

Klíčová slova anglicky

PRIMARY CILIA; CEP164; TTBK2; PHOSPHORYLATION; DIFFERENTIATION; PROLIFERATION; SPECIFICATION

Štítky

14110517, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 8. 4. 2024 10:43, Mgr. Tereza Miškechová

Anotace

V originále

Primary cilia are key regulators of embryo development and tissue homeostasis. However, their mechanisms and functions, particularly in the context of human cells, are still unclear. Here, we analyzed the consequences of primary cilia modulation for human pluripotent stem cells (hPSCs) proliferation and differentiation. We report that neither activation of the cilia-associated Hedgehog signaling pathway nor ablation of primary cilia by CRISPR gene editing to knockout Tau Tubulin Kinase 2 (TTBK2), a crucial ciliogenesis regulator, affects the self-renewal of hPSCs. Further, we show that TTBK1, a related kinase without previous links to ciliogenesis, is upregulated during hPSCs-derived neural rosette differentiation. Importantly, we demonstrate that while TTBK1 fails to localize to the mother centriole, it regulates primary cilia formation in the differentiated, but not the undifferentiated hPSCs. Finally, we show that TTBK1/2 and primary cilia are implicated in the regulation of the size of hPSCs-derived neural rosettes.

Návaznosti

GA22-13277S, projekt VaV

Název: Ciliární Tau tubulin kináza 2 ve zdraví a nemoci: struktura a funkce

Investor: Grantová agentura ČR, Ciliární Tau tubulin kináza 2 ve zdraví a nemoci: struktura a funkce

LM2018129, projekt VaV

Název: Národní infrastruktura pro biologické a medicínské zobrazování Czech-BioImaging

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, National research infrastructure for biological and medical imaging

MUNI/11/SUP/03/2022, interní kód MU

Název: Modelace ztráty ciliární funkce pomocí lidských pluripotentních buněk

Investor: Masarykova univerzita, Modelace ztráty ciliární funkce pomocí lidských pluripotentních buněk, Start-up

90250, velká výzkumná infrastruktura

Název: Czech-BioImaging III

Citovat

BINÓ, Lucia a Lukáš ČAJÁNEK. Tau tubulin kinase 1 and 2 regulate ciliogenesis and human pluripotent stem cells-derived neural rosettes. Scientific Reports. Berlin: Nature Research, 2023, roč. 13, č. 1, s. 1-13. ISSN 2045-2322. Dostupné z: https://dx.doi.org/10.1038/s41598-023-39887-9.

@article{2324820,
   author = {Binó, Lucia and Čajánek, Lukáš},
   article_location = {Berlin},
   article_number = {1},
   doi = {http://dx.doi.org/10.1038/s41598-023-39887-9},
   keywords = {PRIMARY CILIA; CEP164; TTBK2; PHOSPHORYLATION; DIFFERENTIATION; PROLIFERATION; SPECIFICATION},
   language = {eng},
   issn = {2045-2322},
   journal = {Scientific Reports},
   title = {Tau tubulin kinase 1 and 2 regulate ciliogenesis and human pluripotent stem cells-derived neural rosettes},
   url = {https://www.nature.com/articles/s41598-023-39887-9},
   volume = {13},
   year = {2023}
}

TY  - JOUR
ID  - 2324820
AU  - Binó, Lucia - Čajánek, Lukáš
PY  - 2023
TI  - Tau tubulin kinase 1 and 2 regulate ciliogenesis and human pluripotent stem cells-derived neural rosettes
JF  - Scientific Reports
VL  - 13
IS  - 1
SP  - 1-13
EP  - 1-13
PB  - Nature Research
SN  - 20452322
KW  - PRIMARY CILIA
KW  - CEP164
KW  - TTBK2
KW  - PHOSPHORYLATION
KW  - DIFFERENTIATION
KW  - PROLIFERATION
KW  - SPECIFICATION
UR  - https://www.nature.com/articles/s41598-023-39887-9
N2  - Primary cilia are key regulators of embryo development and tissue homeostasis. However, their mechanisms and functions, particularly in the context of human cells, are still unclear. Here, we analyzed the consequences of primary cilia modulation for human pluripotent stem cells (hPSCs) proliferation and differentiation. We report that neither activation of the cilia-associated Hedgehog signaling pathway nor ablation of primary cilia by CRISPR gene editing to knockout Tau Tubulin Kinase 2 (TTBK2), a crucial ciliogenesis regulator, affects the self-renewal of hPSCs. Further, we show that TTBK1, a related kinase without previous links to ciliogenesis, is upregulated during hPSCs-derived neural rosette differentiation. Importantly, we demonstrate that while TTBK1 fails to localize to the mother centriole, it regulates primary cilia formation in the differentiated, but not the undifferentiated hPSCs. Finally, we show that TTBK1/2 and primary cilia are implicated in the regulation of the size of hPSCs-derived neural rosettes.
ER  -

BINÓ, Lucia a Lukáš ČAJÁNEK. Tau tubulin kinase 1 and 2 regulate ciliogenesis and human pluripotent stem cells-derived neural rosettes. \textit{Scientific Reports}. Berlin: Nature Research, 2023, roč.~13, č.~1, s.~1-13. ISSN~2045-2322. Dostupné z: https://dx.doi.org/10.1038/s41598-023-39887-9.

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