Molecular Detection of Borrelia burgdorferi s.l. (Borreliella)
and Chlamydia-Like Organism DNA in Early Developmental Stages
of Arthropod Vector Species

J 2023

Molecular Detection of Borrelia burgdorferi s.l. (Borreliella) and Chlamydia-Like Organism DNA in Early Developmental Stages of Arthropod Vector Species

PETRÁŠ, Jiří, Eva BÁRTOVÁ a Alena ŽÁKOVSKÁ

Základní údaje

Originální název

Molecular Detection of Borrelia burgdorferi s.l. (Borreliella) and Chlamydia-Like Organism DNA in Early Developmental Stages of Arthropod Vector Species

Autoři

PETRÁŠ, Jiří (203 Česká republika, garant, domácí), Eva BÁRTOVÁ (203 Česká republika) a Alena ŽÁKOVSKÁ (203 Česká republika, domácí)

Vydání

Transboundary and Emerging Diseases, Wiley, 2023, 1865-1674

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.300 v roce 2022

Kód RIV

RIV/00216224:14410/23:00131925

Organizační jednotka

Pedagogická fakulta

DOI

http://dx.doi.org/10.1155/2023/2511753

UT WoS

001090824800002

Klíčová slova anglicky

Bbsl; Chlamydiales; Culex; Ixodes; Lipoptena; Parachlamydiaceae; transgenerational; transovarial

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 23. 1. 2024 10:11, Mgr. Daniela Marcollová

Anotace

V originále

Borrelia burgdorferi sensu lato (Bbsl) is spirochetes transmitted by ticks and known to cause Lyme disease. Chlamydia-like organisms (CLOs) comprise a large group of bacteria that can lead to serious health disorders, including miscarriage. Recently, CLOs have been found in ticks and patient skin biopsies. Due to the involvement of multiple potential vectors in the spread of these pathogens, the objective of this study was to confirm the presence of both organisms in the early developmental stages of selected vectors. Three potential vectors, Ixodes ricinus larvae, Culex pipiens larvae, and winged (unfed) adults of Lipoptena cervi, were collected in the Czech Republic in years 2019–2020. The presence of Bbsl and panchlamydial DNA was detected by PCR and positive samples were further analyzed by Sanger sequencing and phylogenetic tree construction. Bbsl DNA was proved in 1.5% (2/137) of I. ricinus larvae (identified as Borrelia afzelii and Borrelia garinii), in 1.7% (2/119) of C. pipiens larvae (both identified as B. garinii), and in 11% (3/27) of L. cervi (all identified as B. garinii). CLOs were identified in 0.7% (1/137) of I. ricinus larvae (Candidatus Protochlamydia) and in 7.4% (2/27) of L. cervi (unspecified genus), while C. pipiens larvae could not be evaluated (0%). This research represents the first investigation of the presence of CLOs in L. cervi. The detection of pathogen DNA in the early developmental stages of vectors suggests the potential for transgenerational transmission of Bbsl and CLOs in the selected vectors, although at a low rate.

Návaznosti

MUNI/A/1258/2022, interní kód MU

Název: Podpora výuky a výzkumné činnosti studentů v oblasti fyziologie, vývojové biologie a imunologie živočichů 2023

Investor: Masarykova univerzita, Podpora výuky a výzkumné činnosti studentů v oblasti fyziologie, vývojové biologie a imunologie živočichů 2023

Citovat

PETRÁŠ, Jiří, Eva BÁRTOVÁ a Alena ŽÁKOVSKÁ. Molecular Detection of Borrelia burgdorferi s.l. (Borreliella) and Chlamydia-Like Organism DNA in Early Developmental Stages of Arthropod Vector Species. Transboundary and Emerging Diseases. Wiley, 2023, roč. 2023, article ID 2511753, s. 1-8. ISSN 1865-1674. Dostupné z: https://dx.doi.org/10.1155/2023/2511753.

@article{2326637,
   author = {Petráš, Jiří and Bártová, Eva and Žákovská, Alena},
   article_number = {article ID 2511753},
   doi = {http://dx.doi.org/10.1155/2023/2511753},
   keywords = {Bbsl; Chlamydiales; Culex; Ixodes; Lipoptena; Parachlamydiaceae; transgenerational; transovarial},
   language = {eng},
   issn = {1865-1674},
   journal = {Transboundary and Emerging Diseases},
   title = {Molecular Detection of Borrelia burgdorferi s.l. (Borreliella) and Chlamydia-Like Organism DNA in Early Developmental Stages of Arthropod Vector Species},
   url = {https://doi.org/10.1155/2023/2511753},
   volume = {2023},
   year = {2023}
}

TY  - JOUR
ID  - 2326637
AU  - Petráš, Jiří - Bártová, Eva - Žákovská, Alena
PY  - 2023
TI  - Molecular Detection of Borrelia burgdorferi s.l. (Borreliella) and Chlamydia-Like Organism DNA in Early Developmental Stages of Arthropod Vector Species
JF  - Transboundary and Emerging Diseases
VL  - 2023
IS  - article ID 2511753
SP  - 1-8
EP  - 1-8
PB  - Wiley
SN  - 18651674
KW  - Bbsl
KW  - Chlamydiales
KW  - Culex
KW  - Ixodes
KW  - Lipoptena
KW  - Parachlamydiaceae
KW  - transgenerational
KW  - transovarial
UR  - https://doi.org/10.1155/2023/2511753
N2  - Borrelia burgdorferi sensu lato (Bbsl) is spirochetes transmitted by ticks and known to cause Lyme disease. Chlamydia-like organisms (CLOs) comprise a large group of bacteria that can lead to serious health disorders, including miscarriage. Recently, CLOs have been found in ticks and patient skin biopsies. Due to the involvement of multiple potential vectors in the spread of these pathogens, the objective of this study was to confirm the presence of both organisms in the early developmental stages of selected vectors. Three potential vectors, Ixodes ricinus larvae, Culex pipiens larvae, and winged (unfed) adults of Lipoptena cervi, were collected in the Czech Republic in years 2019–2020. The presence of Bbsl and panchlamydial DNA was detected by PCR and positive samples were further analyzed by Sanger sequencing and phylogenetic tree construction. Bbsl DNA was proved in 1.5% (2/137) of I. ricinus larvae (identified as Borrelia afzelii and Borrelia garinii), in 1.7% (2/119) of C. pipiens larvae (both identified as B. garinii), and in 11% (3/27) of L. cervi (all identified as B. garinii). CLOs were identified in 0.7% (1/137) of I. ricinus larvae (Candidatus Protochlamydia) and in 7.4% (2/27) of L. cervi (unspecified genus), while C. pipiens larvae could not be evaluated (0%). This research represents the first investigation of the presence of CLOs in L. cervi. The detection of pathogen DNA in the early developmental stages of vectors suggests the potential for transgenerational transmission of Bbsl and CLOs in the selected vectors, although at a low rate.
ER  -

PETRÁŠ, Jiří, Eva BÁRTOVÁ a Alena ŽÁKOVSKÁ. Molecular Detection of Borrelia burgdorferi s.l. (Borreliella) and Chlamydia-Like Organism DNA in Early Developmental Stages of Arthropod Vector Species. \textit{Transboundary and Emerging Diseases}. Wiley, 2023, roč.~2023, article ID 2511753, s.~1-8. ISSN~1865-1674. Dostupné z: https://dx.doi.org/10.1155/2023/2511753.

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