PLAUR alternative splice pattern analysis during
monocyte-to-macrophage differentiation in healthy volunteers
and patients with rheumatoid arthritis and bronchial asthma

a 2023

PLAUR alternative splice pattern analysis during monocyte-to-macrophage differentiation in healthy volunteers and patients with rheumatoid arthritis and bronchial asthma

KULÍŠKOVÁ, Petra, Lucie BALLONOVÁ, Peter SLANINA, Marcela VLKOVÁ, Jiří LITZMAN et. al.

Základní údaje

Originální název

PLAUR alternative splice pattern analysis during monocyte-to-macrophage differentiation in healthy volunteers and patients with rheumatoid arthritis and bronchial asthma

Název česky

Analýza alternativního sestřihu PLAUR během diferenciace monocytů na makrofágy u zdravých dobrovolníků a pacientů s revmatoidní artritidou a bronchiálním astmatem

Autoři

KULÍŠKOVÁ, Petra (203 Česká republika, garant, domácí), Lucie BALLONOVÁ (203 Česká republika, domácí), Peter SLANINA (703 Slovensko, domácí), Marcela VLKOVÁ (203 Česká republika, domácí), Jiří LITZMAN (203 Česká republika, domácí), Petr NĚMEC (203 Česká republika, domácí), Jan BAROŠ (203 Česká republika, domácí), Přemysl SOUČEK (203 Česká republika, domácí) a Tomáš FREIBERGER (203 Česká republika, domácí)

Vydání

5th International Caparica Conference in SPLICING 2023, 2023

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

30102 Immunology

Stát vydavatele

Portugalsko

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14110/23:00134659

Organizační jednotka

Lékařská fakulta

Klíčová slova česky

PLAUR; diferenciace; revmatoidní artritida; bronchiální asthma

Klíčová slova anglicky

PLAUR; differentiation; rheumatoid arthritis; bronchial asthma

Příznaky

Mezinárodní význam

Změněno: 22. 4. 2024 11:16, Mgr. Tereza Miškechová

Anotace

V originále

PLAUR gene, which encodes multiligand receptor uPAR, may undergo an alternative splicing producing PLAUR splice variants differing in the presence or absence of cassette exons 5 and 6 in final transcripts, leading to production of the shortened uPAR variants. In rheumatoid arthritis (RA) and bronchial asthma (BA), uPAR plays an important role, however the impact of uPAR splicing variatns on the disease development and severity has not been investigated yet. We analysed the PLAUR splicing pattern changes in RA and BA patients´ monocytes and macrophages with both mild and severe clinical phenotype and compare with healthy individuals. The results to this date indicates potential changes in variant frequency encoding full form of suPAR in monocytes of RA patients compared to monocytes isolated from BA patients and healthy volunteers. Another change in the splicing pattern is observed for the variant frequency encoding the ΔE5+6 membrane-bound uPAR in monocytes of BA patients compared to monocytes isolated from RA patients and healthy volunteers. In all tested groups, the decreasing character in alternatively spliced isoforms proportion during monocyte-to-macrophage differentiation can be seen. Due to important role of both macrophages and monocytes in RA and BA, the expression profile of uPAR could have an impact on disease course an/or it could be an important marker of their inflammatory status.

Návaznosti

MUNI/A/1098/2022, interní kód MU

Název: Nespecifická imunita u chorob imunitního systému

Investor: Masarykova univerzita, Nespecifická imunita u chorob imunitního systému

NU21-05-00438, projekt VaV

Název: Úloha alternativních forem uPAR v rozvoji imunopatologických reakcí

Investor: Ministerstvo zdravotnictví ČR, Úloha alternativních forem uPAR v rozvoji imunopatologických reakcí, Podprogram 1 - standardní

Citovat

KULÍŠKOVÁ, Petra, Lucie BALLONOVÁ, Peter SLANINA, Marcela VLKOVÁ, Jiří LITZMAN, Petr NĚMEC, Jan BAROŠ, Přemysl SOUČEK a Tomáš FREIBERGER. PLAUR alternative splice pattern analysis during monocyte-to-macrophage differentiation in healthy volunteers and patients with rheumatoid arthritis and bronchial asthma. In 5th International Caparica Conference in SPLICING 2023. 2023.

@proceedings{2330797,
   author = {Kulíšková, Petra and Ballonová, Lucie and Slanina, Peter and Vlková, Marcela and Litzman, Jiří and Němec, Petr and Baroš, Jan and Souček, Přemysl and Freiberger, Tomáš},
   booktitle = {5th International Caparica Conference in SPLICING 2023},
   keywords = {PLAUR; differentiation; rheumatoid arthritis; bronchial asthma},
   language = {eng},
   title = {PLAUR alternative splice pattern analysis during monocyte-to-macrophage differentiation in healthy volunteers and patients with rheumatoid arthritis and bronchial asthma},
   year = {2023}
}

TY  - CONF
ID  - 2330797
AU  - Kulíšková, Petra - Ballonová, Lucie - Slanina, Peter - Vlková, Marcela - Litzman, Jiří - Němec, Petr - Baroš, Jan - Souček, Přemysl - Freiberger, Tomáš
PY  - 2023
TI  - PLAUR alternative splice pattern analysis during monocyte-to-macrophage differentiation in healthy volunteers and patients with rheumatoid arthritis and bronchial asthma
KW  - PLAUR
KW  - differentiation
KW  - rheumatoid arthritis
KW  - bronchial asthma
N2  - PLAUR gene, which encodes multiligand receptor uPAR, may undergo an alternative splicing producing PLAUR splice variants differing in the presence or absence of cassette exons 5 and 6 in final transcripts, leading to production of the shortened uPAR variants. In rheumatoid arthritis (RA) and bronchial asthma (BA), uPAR plays an important role, however the impact of uPAR splicing variatns on the disease development and severity has not been investigated yet. We analysed the PLAUR splicing pattern changes in RA and BA patients´ monocytes and macrophages with both mild and severe clinical phenotype and compare with healthy individuals. The results to this date indicates potential changes in variant frequency encoding full form of suPAR in monocytes of RA patients compared to monocytes isolated from BA patients and healthy volunteers. Another change in the splicing pattern is observed for the variant frequency encoding the ΔE5+6 membrane-bound uPAR in monocytes of BA patients compared to monocytes isolated from RA patients and healthy volunteers. In all tested groups, the decreasing character in alternatively spliced isoforms proportion during monocyte-to-macrophage differentiation can be seen. Due to important role of both macrophages and monocytes in RA and BA, the expression profile of uPAR could have an impact on disease course an/or it could be an important marker of their inflammatory status.
ER  -

KULÍŠKOVÁ, Petra, Lucie BALLONOVÁ, Peter SLANINA, Marcela VLKOVÁ, Jiří LITZMAN, Petr NĚMEC, Jan BAROŠ, Přemysl SOUČEK a Tomáš FREIBERGER. PLAUR alternative splice pattern analysis during monocyte-to-macrophage differentiation in healthy volunteers and patients with rheumatoid arthritis and bronchial asthma. In \textit{5th International Caparica Conference in SPLICING 2023}. 2023.

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