2023
Bioactive molecules released by Heterorhabditis bacteriophora in response to various activation materials
ŠREIBR, Sara, Jana ILGOVÁ, Pavel DOBEŠ, Jiří VOREL, Jacek MARCINIAK et. al.Základní údaje
Originální název
Bioactive molecules released by Heterorhabditis bacteriophora in response to various activation materials
Název česky
Bioaktivní molekuly uvolňované Heterorhabditis bacteriophora v reakci na různé aktivační materiály
Autoři
ŠREIBR, Sara (276 Německo, domácí), Jana ILGOVÁ (703 Slovensko, domácí), Pavel DOBEŠ (203 Česká republika, domácí), Jiří VOREL (203 Česká republika, domácí), Jacek MARCINIAK (203 Česká republika, domácí), Jana HURYCHOVÁ (203 Česká republika, domácí), Martin KAŠNÝ (203 Česká republika, domácí) a Pavel HYRŠL (203 Česká republika, domácí)
Vydání
Virtual Nematology Conference 2023, 2023
Další údaje
Jazyk
angličtina
Typ výsledku
Konferenční abstrakt
Obor
10616 Entomology
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Kód RIV
RIV/00216224:14310/23:00134270
Organizační jednotka
Přírodovědecká fakulta
Klíčová slova česky
Bioaktivní molekuly; Heterorhabditis bacteriophora; aktivace; aktivační materiály
Klíčová slova anglicky
Bioactive molecules; Heterorhabditis bacteriophora; activation; activation materials
Změněno: 1. 11. 2023 15:06, Mgr. Sara Šreibr
V originále
Entomopathogenic nematodes (EPNs) are obligate insect parasites commonly used in the biocontrol of many insect pests. They produce bioactive molecules referred to as excreted/secreted products (ESPs). The ESPs can interfere with the host immune system, increasing infection efficiency and chances of EPNs survival and reproduction. Therefore, understanding the composition and function of ESPs is vital for unraveling the mechanisms underlying nematode pathogenicity and improving their efficient usage as biocontrol agents. Our aim was to assess the effect of various activation materials on the ESPs protein spectrum and to identify infection-specific molecules by comparison with the ESPs profiles of infective juveniles treated with water or phosphate buffer. Three activation materials prepared from larvae of Galleria mellonella, and two controls of water and phosphate buffer were used for the in vitro activation of Heterorhabditis bacteriophora infective juveniles. The produced ESPs were collected and subjected to LC-MS/MS analysis using RSLCNano system on-line connected to Impact II Ultra-High Resolution Qq-Time-Of-Flight mass spectrometer. Protein searches were performed against a protein database derived from H. bacteriophora reference genome. LC-MS/MS results revealed significant variations in the composition of ESPs following activation with different materials. We identified several proteins, e.g. ShTK domain proteins, fatty acid- and retinoid- binding protein, recently associated with pathogenicity and immune modulation. The activation materials influenced the abundance and diversity of ESP components, suggesting that these materials may play a crucial role in modulating the nematode's virulence. This study was supported by grant GAČR 23-06457S.
Česky
Entomopathogenic nematodes (EPNs) are obligate insect parasites commonly used in the biocontrol of many insect pests. They produce bioactive molecules referred to as excreted/secreted products (ESPs). The ESPs can interfere with the host immune system, increasing infection efficiency and chances of EPNs survival and reproduction. Therefore, understanding the composition and function of ESPs is vital for unraveling the mechanisms underlying nematode pathogenicity and improving their efficient usage as biocontrol agents. Our aim was to assess the effect of various activation materials on the ESPs protein spectrum and to identify infection-specific molecules by comparison with the ESPs profiles of infective juveniles treated with water or phosphate buffer. Three activation materials prepared from larvae of Galleria mellonella, and two controls of water and phosphate buffer were used for the in vitro activation of Heterorhabditis bacteriophora infective juveniles. The produced ESPs were collected and subjected to LC-MS/MS analysis using RSLCNano system on-line connected to Impact II Ultra-High Resolution Qq-Time-Of-Flight mass spectrometer. Protein searches were performed against a protein database derived from H. bacteriophora reference genome. LC-MS/MS results revealed significant variations in the composition of ESPs following activation with different materials. We identified several proteins, e.g. ShTK domain proteins, fatty acid- and retinoid- binding protein, recently associated with pathogenicity and immune modulation. The activation materials influenced the abundance and diversity of ESP components, suggesting that these materials may play a crucial role in modulating the nematode's virulence. This study was supported by grant GAČR 23-06457S.
Návaznosti
| GA23-06457S, projekt VaV |
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