Bioactive molecules released by Heterorhabditis bacteriophora in response to various activation materials

ŠREIBR, Sara, Jana ILGOVÁ, Pavel DOBEŠ, Jiří VOREL, Jacek MARCINIAK, Jana HURYCHOVÁ, Martin KAŠNÝ a Pavel HYRŠL. Bioactive molecules released by Heterorhabditis bacteriophora in response to various activation materials. In Virtual Nematology Conference 2023. 2023.

Základní údaje

• Originální název: Bioactive molecules released by Heterorhabditis bacteriophora in response to various activation materials
• Název česky: Bioaktivní molekuly uvolňované Heterorhabditis bacteriophora v reakci na různé aktivační materiály
• Autoři: ŠREIBR, Sara (276 Německo, domácí), Jana ILGOVÁ (703 Slovensko, domácí), Pavel DOBEŠ (203 Česká republika, domácí), Jiří VOREL (203 Česká republika, domácí), Jacek MARCINIAK (203 Česká republika, domácí), Jana HURYCHOVÁ (203 Česká republika, domácí), Martin KAŠNÝ (203 Česká republika, domácí) a Pavel HYRŠL (203 Česká republika, domácí).
• Vydání: Virtual Nematology Conference 2023, 2023.

Další údaje

• Originální jazyk: angličtina
• Typ výsledku: Konferenční abstrakt
• Obor: 10616 Entomology
• Stát vydavatele: Česká republika
• Utajení: není předmětem státního či obchodního tajemství
• Kód RIV: RIV/00216224:14310/23:00134270
• Organizační jednotka: Přírodovědecká fakulta
• Klíčová slova česky: Bioaktivní molekuly; Heterorhabditis bacteriophora; aktivace; aktivační materiály
• Klíčová slova anglicky: Bioactive molecules; Heterorhabditis bacteriophora; activation; activation materials

Anotace

Entomopathogenic nematodes (EPNs) are obligate insect parasites commonly used in the biocontrol of many insect pests. They produce bioactive molecules referred to as excreted/secreted products (ESPs). The ESPs can interfere with the host immune system, increasing infection efficiency and chances of EPNs survival and reproduction. Therefore, understanding the composition and function of ESPs is vital for unraveling the mechanisms underlying nematode pathogenicity and improving their efficient usage as biocontrol agents. Our aim was to assess the effect of various activation materials on the ESPs protein spectrum and to identify infection-specific molecules by comparison with the ESPs profiles of infective juveniles treated with water or phosphate buffer. Three activation materials prepared from larvae of Galleria mellonella, and two controls of water and phosphate buffer were used for the in vitro activation of Heterorhabditis bacteriophora infective juveniles. The produced ESPs were collected and subjected to LC-MS/MS analysis using RSLCNano system on-line connected to Impact II Ultra-High Resolution Qq-Time-Of-Flight mass spectrometer. Protein searches were performed against a protein database derived from H. bacteriophora reference genome. LC-MS/MS results revealed significant variations in the composition of ESPs following activation with different materials. We identified several proteins, e.g. ShTK domain proteins, fatty acid- and retinoid- binding protein, recently associated with pathogenicity and immune modulation. The activation materials influenced the abundance and diversity of ESP components, suggesting that these materials may play a crucial role in modulating the nematode's virulence. This study was supported by grant GAČR 23-06457S.

Anotace česky

Entomopathogenic nematodes (EPNs) are obligate insect parasites commonly used in the biocontrol of many insect pests. They produce bioactive molecules referred to as excreted/secreted products (ESPs). The ESPs can interfere with the host immune system, increasing infection efficiency and chances of EPNs survival and reproduction. Therefore, understanding the composition and function of ESPs is vital for unraveling the mechanisms underlying nematode pathogenicity and improving their efficient usage as biocontrol agents. Our aim was to assess the effect of various activation materials on the ESPs protein spectrum and to identify infection-specific molecules by comparison with the ESPs profiles of infective juveniles treated with water or phosphate buffer. Three activation materials prepared from larvae of Galleria mellonella, and two controls of water and phosphate buffer were used for the in vitro activation of Heterorhabditis bacteriophora infective juveniles. The produced ESPs were collected and subjected to LC-MS/MS analysis using RSLCNano system on-line connected to Impact II Ultra-High Resolution Qq-Time-Of-Flight mass spectrometer. Protein searches were performed against a protein database derived from H. bacteriophora reference genome. LC-MS/MS results revealed significant variations in the composition of ESPs following activation with different materials. We identified several proteins, e.g. ShTK domain proteins, fatty acid- and retinoid- binding protein, recently associated with pathogenicity and immune modulation. The activation materials influenced the abundance and diversity of ESP components, suggesting that these materials may play a crucial role in modulating the nematode's virulence. This study was supported by grant GAČR 23-06457S.

Návaznosti

• GA23-06457S, projekt VaV: Název: Identifikace a funkční charakteristika bioaktivních molekul produkovaných entomopatogenními hlísticemi

Investor: Grantová agentura ČR, Identifikace a funkční charakteristika bioaktivních molekul produkovaných entomopatogenními hlísticemi