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@proceedings{2333417,
author = {Král, Martin and Švecová, Olga and Zelenák, Štefan and Pacherník, Jiří and Bárta, Tomáš and Lietava, Samuel and Synková, Iva and Zídková, Jana and Novotný, Tomáš and Bébarová, Markéta},
booktitle = {47th Meeting of the European Working Group on Cardiac Cellular Electrophysiology, 2023},
keywords = {RYR2; idiopathic ventricular fibrillation; inhereted arrhythmia; hiPSC; MEA},
language = {eng},
title = {Spontaneous electrical activity of cardiomyocytes derived from hiPSCs of a patient with idiopathic ventricular fibrillation and RYR2 variant: impact of isoprenaline and varying concentrations of Ca2+},
url = {https://escardio.sharepoint.com/sites/ESCEvents/Shared%20Documents/Forms/AllItems.aspx?id=%2Fsites%2FESCEvents%2FShared%20Documents%2FPublic%20shared%20documents%2FWorking%20group%20Events%2F2023%2FEWGCCE%20Meeting%202023%2FEWGCCE%5F2023%5Fabstract%5Fboo},
year = {2023}
}
TY - CONF
ID - 2333417
AU - Král, Martin - Švecová, Olga - Zelenák, Štefan - Pacherník, Jiří - Bárta, Tomáš - Lietava, Samuel - Synková, Iva - Zídková, Jana - Novotný, Tomáš - Bébarová, Markéta
PY - 2023
TI - Spontaneous electrical activity of cardiomyocytes derived from hiPSCs of a patient with idiopathic ventricular fibrillation and RYR2 variant: impact of isoprenaline and varying concentrations of Ca2+
KW - RYR2
KW - idiopathic ventricular fibrillation
KW - inhereted arrhythmia
KW - hiPSC
KW - MEA
UR - https://escardio.sharepoint.com/sites/ESCEvents/Shared%20Documents/Forms/AllItems.aspx?id=%2Fsites%2FESCEvents%2FShared%20Documents%2FPublic%20shared%20documents%2FWorking%20group%20Events%2F2023%2FEWGCCE%20Meeting%202023%2FEWGCCE%5F2023%5Fabstract%5Fboo
N2 - Background: We have found a potentially proarrhythmic variant p. Y4734C in the ryanodine receptor type 2 (RYR2) in a patient suffering from idiopathic ventricular fibrillation. Here we bring pilot data showing the effect of 0.1 µM isoprenaline (ISO) and 0.5-5 mM Ca2+ on the spontaneous electrical activity of patient-specific cardiomyocytes derived from human-induced pluripotent stem cells (hiPSC-CM). Material and Methods: The spontaneous electrical activity of Y4734C-RYR2 hiPSC-CM (Y4734C) and hiPSC-CM of unrelated healthy control (WT) was recorded using the multielectrode array (MEA) technique at 37 °C. The cycle length (CL) and field potential duration (FPD and FPDc – correction according to Fridericia) are presented as the median ± interquartile range (IQR); non-parametric tests were used for comparison. Results: In control conditions (1.8 mM Ca2+), CL was insignificantly longer in WT (5.36±5.87 s; n=9) than in Y4734C (1.22±0.82 s; n=6). FDP and FPDc were significantly longer in WT (0.35±0.51 and 0.16±0.13 s, respectively; n=9) than in Y4734C (0.08±0.04 and 0.09±0.05 s, respectively; n=6; P˂0.05). Under the effect of 0.1 µM ISO, CL was shortened by 45.0±16.7% (n=9, P˂0.05) in WT and a similar, but insignificant change was observed in Y4734C (by 24.9±29.6%, n=5). We detected a significant CL prolongation in the presence of 1, 2, 3, and 5 mM Ca2+ in WT (P˂0.05) whereas it was not significantly changed in Y4734C. No significant changes in FPD and FPDc were observed both in ISO and varying concentrations of Ca2+ except for a shortened FPD in 0.1 µM ISO in WT. Conclusions: The pilot data showed longer FPD and FPDc in WT vs. Y4734C and significant changes mostly in CL in WT at 0.1 µM ISO and various Ca2+ concentrations.
ER -
KRÁL, Martin, Olga ŠVECOVÁ, Štefan ZELENÁK, Jiří PACHERNÍK, Tomáš BÁRTA, Samuel LIETAVA, Iva SYNKOVÁ, Jana ZÍDKOVÁ, Tomáš NOVOTNÝ a Markéta BÉBAROVÁ. Spontaneous electrical activity of cardiomyocytes derived from hiPSCs of a patient with idiopathic ventricular fibrillation and RYR2 variant: impact of isoprenaline and varying concentrations of Ca2+. In \textit{47th Meeting of the European Working Group on Cardiac Cellular Electrophysiology, 2023}. 2023.
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