N6-adenosine methylation of mRNA integrates multilevel auxin
response and ground tissue development in Arabidopsis

J 2023

N6-adenosine methylation of mRNA integrates multilevel auxin response and ground tissue development in Arabidopsis

ZEMLYANSKAYA, Elena, Viacheslav ZEMLIANSKI, Aleš PĚNČÍK, Dior R. KELLEY, Ykä HELARIUTTA et. al.

Základní údaje

Originální název

N6-adenosine methylation of mRNA integrates multilevel auxin response and ground tissue development in Arabidopsis

Autoři

ZEMLYANSKAYA, Elena (112 Bělorusko, domácí), Viacheslav ZEMLIANSKI, Aleš PĚNČÍK, Dior R. KELLEY, Ykä HELARIUTTA, Ondřej NOVÁK a Kamil RŮŽIČKA (203 Česká republika, garant)

Vydání

Development, The Company of Biologists Ltd, 2023, 0950-1991

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10605 Developmental biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.600 v roce 2022

Kód RIV

RIV/00216224:14740/23:00134766

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1242/dev.201775

UT WoS

001092309300002

Klíčová slova anglicky

m6A; mRNA methylation; Auxin; Endodermis; SAUR; Arabidopsis

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 8. 2024 10:17, Mgr. Eva Dubská

Anotace

V originále

N6-methyl adenosine (m6A) is a widespread internal mRNA modification impacting the expression of numerous genes. Here, we characterize auxin-related defects among the pleiotropic phenotypes of hypomorphic Arabidopsis thaliana mutants with impaired m6A status and reveal that they show strong resistance to exogenously applied auxin. By combining major published m6A datasets, we propose that among high-confidence target transcripts emerge those encoding the main components required for auxin signaling, including the TIR1/AFB auxin receptors and ARF transcriptional regulators. We also observe subtle changes in endogenous levels of indole-3-acetic acid metabolites in these hypomorphic lines, which correlate with the methylation status of indole-3-acetic acid amidohydrolase transcripts. In addition, we reveal that reduced m6A levels lead to defects in endodermal patterning in the primary root arising from impaired timing of periclinal cell divisions. These defects can be reverted by inhibition of auxin signaling. Together, our data underline that m6A likely affects auxin-dependent processes at multiple levels.

Návaznosti

EF18_046/0016045, projekt VaV

Název: Modernizace národní infrastruktury pro biologické a medicínské zobrazování Czech-BioImaging

LM2023050, projekt VaV

Název: Národní infrastruktura pro biologické a medicínské zobrazování

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech BioImaging: Národní výzkumná infrastruktura pro biologické a medicínské zobrazování

Citovat

ZEMLYANSKAYA, Elena, Viacheslav ZEMLIANSKI, Aleš PĚNČÍK, Dior R. KELLEY, Ykä HELARIUTTA, Ondřej NOVÁK a Kamil RŮŽIČKA. N6-adenosine methylation of mRNA integrates multilevel auxin response and ground tissue development in Arabidopsis. Development. The Company of Biologists Ltd, 2023, roč. 150, č. 19, s. 1-11. ISSN 0950-1991. Dostupné z: https://dx.doi.org/10.1242/dev.201775.

@article{2333939,
   author = {Zemlyanskaya, Elena and Zemlianski, Viacheslav and Pěnčík, Aleš and Kelley, Dior R. and Helariutta, Ykä and Novák, Ondřej and Růžička, Kamil},
   article_number = {19},
   doi = {http://dx.doi.org/10.1242/dev.201775},
   keywords = {m6A; mRNA methylation; Auxin; Endodermis; SAUR; Arabidopsis},
   language = {eng},
   issn = {0950-1991},
   journal = {Development},
   title = {N6-adenosine methylation of mRNA integrates multilevel auxin response and ground tissue development in Arabidopsis},
   url = {https://doi.org/10.1242/dev.201775},
   volume = {150},
   year = {2023}
}

TY  - JOUR
ID  - 2333939
AU  - Zemlyanskaya, Elena - Zemlianski, Viacheslav - Pěnčík, Aleš - Kelley, Dior R. - Helariutta, Ykä - Novák, Ondřej - Růžička, Kamil
PY  - 2023
TI  - N6-adenosine methylation of mRNA integrates multilevel auxin response and ground tissue development in Arabidopsis
JF  - Development
VL  - 150
IS  - 19
SP  - 1-11
EP  - 1-11
PB  - The Company of Biologists Ltd
SN  - 09501991
KW  - m6A
KW  - mRNA methylation
KW  - Auxin
KW  - Endodermis
KW  - SAUR
KW  - Arabidopsis
UR  - https://doi.org/10.1242/dev.201775
N2  - N6-methyl adenosine (m6A) is a widespread internal mRNA modification impacting the expression of numerous genes. Here, we characterize auxin-related defects among the pleiotropic phenotypes of hypomorphic Arabidopsis thaliana mutants with impaired m6A status and reveal that they show strong resistance to exogenously applied auxin. By combining major published m6A datasets, we propose that among high-confidence target transcripts emerge those encoding the main components required for auxin signaling, including the TIR1/AFB auxin receptors and ARF transcriptional regulators. We also observe subtle changes in endogenous levels of indole-3-acetic acid metabolites in these hypomorphic lines, which correlate with the methylation status of indole-3-acetic acid amidohydrolase transcripts. In addition, we reveal that reduced m6A levels lead to defects in endodermal patterning in the primary root arising from impaired timing of periclinal cell divisions. These defects can be reverted by inhibition of auxin signaling. Together, our data underline that m6A likely affects auxin-dependent processes at multiple levels.
ER  -

ZEMLYANSKAYA, Elena, Viacheslav ZEMLIANSKI, Aleš PĚNČÍK, Dior R. KELLEY, Ykä HELARIUTTA, Ondřej NOVÁK a Kamil RŮŽIČKA. N6-adenosine methylation of mRNA integrates multilevel auxin response and ground tissue development in Arabidopsis. \textit{Development}. The Company of Biologists Ltd, 2023, roč.~150, č.~19, s.~1-11. ISSN~0950-1991. Dostupné z: https://dx.doi.org/10.1242/dev.201775.

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