Viral transcription-translation coupling in mammalian cells

a 2023

Viral transcription-translation coupling in mammalian cells

DAS, Amiyaranjan, Julia BARTULI, Clemens GRIMM, Utz FISCHER, Gabriel DEMO et. al.

Základní údaje

Originální název

Viral transcription-translation coupling in mammalian cells

Autoři

DAS, Amiyaranjan (356 Indie, domácí), Julia BARTULI (276 Německo), Clemens GRIMM (276 Německo), Utz FISCHER (276 Německo) a Gabriel DEMO (703 Slovensko, garant, domácí)

Vydání

XIX Discussions in Structural Molecular Biology, 2023

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Kód RIV

RIV/00216224:14740/23:00132138

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

translation ribosome cryoEM virus

Štítky

rivok

Změněno: 27. 3. 2024 10:50, Mgr. Eva Dubská

Anotace

V originále

The coupling of transcription and translation (CTT) controls the gene regulation in bacteria. Recent cryo-electron microscopy (cryo-EM) studies showed the physical coupling of these two processes. Interestingly, the double-strand DNA Vaccinia virus (VACV) performs the viral genome replication, transcription, translation, and assembly of virions in infected mammalian cells within discrete cytoplasmic foci called viral factories. The initial rounds of viral transcription and translation during the early phase of infection occur inside the host cytoplasm. In intermediate and late-phase of infection the viral gene expression is carried out inside the viral factories in close association with host ribosomes. Here, we seek to uncover the potential regulation mechanism of viral gene expression via viral-host CTT. The primary approach is to reconstitute the CTT in vitro and use single particle cryo-EM to uncover the detailed view of the structural architecture of the viral-host CTT. We aim to employ cryo-electron tomography and correlated light and electron microscopy (CLEM) to directly visualize the viral factories in a near-native state at a sub-nanometer resolution to confirm the existence of viral-host CTT directly in VACV-infected cells.

Návaznosti

LL2008, projekt VaV

Název: Komunikace mezi transkripcí a translací (Akronym: CROSSBETT)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Komunikace mezi transkripcí a translací

Citovat

DAS, Amiyaranjan, Julia BARTULI, Clemens GRIMM, Utz FISCHER a Gabriel DEMO. Viral transcription-translation coupling in mammalian cells. In XIX Discussions in Structural Molecular Biology. 2023.

@proceedings{2334202,
   author = {Das, Amiyaranjan and Bartuli, Julia and Grimm, Clemens and Fischer, Utz and Demo, Gabriel},
   booktitle = {XIX Discussions in Structural Molecular Biology},
   keywords = {translation ribosome cryoEM virus},
   language = {eng},
   title = {Viral transcription-translation coupling in mammalian cells},
   url = {https://cssb.structbio.org/xix-discussions-in-structural-molecular-biology-and-6th-user-meeting-of-ciisb-czech-infrastructure-for-integrative-structural-biology/},
   year = {2023}
}

TY  - CONF
ID  - 2334202
AU  - Das, Amiyaranjan - Bartuli, Julia - Grimm, Clemens - Fischer, Utz - Demo, Gabriel
PY  - 2023
TI  - Viral transcription-translation coupling in mammalian cells
KW  - translation ribosome cryoEM virus
UR  - https://cssb.structbio.org/xix-discussions-in-structural-molecular-biology-and-6th-user-meeting-of-ciisb-czech-infrastructure-for-integrative-structural-biology/
N2  - The coupling of transcription and translation (CTT) controls the gene regulation in bacteria. Recent cryo-electron microscopy (cryo-EM) studies showed the physical coupling of these two processes. Interestingly, the double-strand DNA Vaccinia virus (VACV) performs the viral genome replication, transcription, translation, and assembly of virions in infected mammalian cells within discrete cytoplasmic foci called viral factories. The initial rounds of viral transcription and translation during the early phase of infection occur inside the host cytoplasm. In intermediate and late-phase of infection the viral gene expression is carried out inside the viral factories in close association with host ribosomes. Here, we seek to uncover the potential regulation mechanism of viral gene expression via viral-host CTT. The primary approach is to reconstitute the CTT in vitro and use single particle cryo-EM to uncover the detailed view of the structural architecture of the viral-host CTT. We aim to employ cryo-electron tomography and correlated light and electron microscopy (CLEM) to directly visualize the viral factories in a near-native state at a sub-nanometer resolution to confirm the existence of viral-host CTT directly in VACV-infected cells.
ER  -

DAS, Amiyaranjan, Julia BARTULI, Clemens GRIMM, Utz FISCHER a Gabriel DEMO. Viral transcription-translation coupling in mammalian cells. In \textit{XIX Discussions in Structural Molecular Biology}. 2023.

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