Angie-LAMP for diagnosis of human eosinophilic meningitis using dog as proxy: A LAMP assay for ...
BALÁŽ, Vojtech, Phoebe RIVORY, Douglas HAYWARD, Susan JAENSCH, Richard MALIK, Rogan LEE, David MODRÝ a Jan ŠLAPETA. Angie-LAMP for diagnosis of human eosinophilic meningitis using dog as proxy: A LAMP assay for Angiostrongylus cantonensis DNA in cerebrospinal fluid. PLOS Neglected Tropical Diseases. San Francisco: Public Library of Science, 2023, roč. 17, č. 5, s. 1-14. ISSN 1935-2735. Dostupné z: https://dx.doi.org/10.1371/journal.pntd.0011038. |
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Základní údaje | |
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Originální název | Angie-LAMP for diagnosis of human eosinophilic meningitis using dog as proxy: A LAMP assay for Angiostrongylus cantonensis DNA in cerebrospinal fluid |
Autoři | BALÁŽ, Vojtech, Phoebe RIVORY, Douglas HAYWARD, Susan JAENSCH, Richard MALIK, Rogan LEE, David MODRÝ (203 Česká republika, garant, domácí) a Jan ŠLAPETA. |
Vydání | PLOS Neglected Tropical Diseases, San Francisco, Public Library of Science, 2023, 1935-2735. |
Další údaje | |
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Originální jazyk | angličtina |
Typ výsledku | Článek v odborném periodiku |
Obor | 30310 Parasitology |
Stát vydavatele | Spojené státy |
Utajení | není předmětem státního či obchodního tajemství |
WWW | URL |
Impakt faktor | Impact factor: 3.800 v roce 2022 |
Kód RIV | RIV/00216224:14310/23:00132146 |
Organizační jednotka | Přírodovědecká fakulta |
Doi | http://dx.doi.org/10.1371/journal.pntd.0011038 |
UT WoS | 000984025300006 |
Klíčová slova anglicky | Angiostrongyliasis; Angiostrongylus; Angiostrongylus cantonensis; Animals; DNA; Ribosomal; Dogs; Humans; LAMP assay; Meningitis; Rats; Snails; Strongylida Infections |
Štítky | rivok |
Příznaky | Mezinárodní význam, Recenzováno |
Změnil | Změnila: Mgr. Marie Šípková, DiS., učo 437722. Změněno: 7. 12. 2023 08:34. |
Anotace |
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Background Angiostrongylus cantonensis (rat lungworm) is recognised as the leading cause of human eosinophilic meningitis, a serious condition observed when nematode larvae migrate through the CNS. Canine Neural Angiostrongyliasis (CNA) is the analogous disease in dogs. Both humans and dogs are accidental hosts, and a rapid diagnosis is warranted. A highly sensitive PCR based assay is available but often not readily accessible in many jurisdictions. An alternative DNA amplification assay that would further improve accessibility is needed. This study aimed to assess the diagnostic utility of a newly designed LAMP assay to detect DNA of globally distributed and invasive A. cantonensis and Angiostrongylus mackerrasae, the other neurotropic Angiostrongylus species, which is native to Australia. Methodology/Principal findings Cerebrospinal fluid (CSF) from dogs with a presumptive diagnosis of A. cantonensis infection (2020–2022) were received for confirmatory laboratory testing and processed for DNA isolation and ultrasensitive Angiostrongylus qPCR targeting AcanR3390. A newly designed LAMP assay targeting the same gene target was directly compared to the reference ultrasensitive qPCR in a diagnostic laboratory setting to determine the presence of A. cantonensis DNA to diagnose CNA. The LAMP assay (Angie-LAMP) allowed the sensitive detection of A. cantonensis DNA from archived DNA specimens (Kappa = 0.81, 95%CI 0.69–0.92; n = 93) and rapid single-step lysis of archived CSF samples (Kappa = 0.77, 95%CI 0.59–0.94; n = 52). Only A. cantonensis DNA was detected in canine CSF samples, and co-infection with A. mackerrasae using amplicon deep sequencing (ITS-2 rDNA) was not demonstrated. Both SYD.1 and AC13 haplotypes were detected using sequencing of partial cox1. Conclusions/Significance The Angie-LAMP assay is a useful molecular tool for detecting Angiostrongylus DNA in canine CSF and performs comparably to a laboratory Angiostrongylus qPCR. Adaptation of single-step sample lysis improved potential applicability for diagnosis of angiostrongyliasis in a clinical setting for dogs and by extension, to humans. |
VytisknoutZobrazeno: 6. 10. 2024 05:21