Changes in body size and fertility due to artificial and
natural feeding of laboratory common bed bugs (Heteroptera:
Cimicidae)

J 2024

Changes in body size and fertility due to artificial and natural feeding of laboratory common bed bugs (Heteroptera: Cimicidae)

SASÍNKOVÁ, Markéta, Jana KŘEMENOVÁ, Petr CHAJMA, Tomáš BARTONIČKA, Christian MASSINO et. al.

Základní údaje

Originální název

Changes in body size and fertility due to artificial and natural feeding of laboratory common bed bugs (Heteroptera: Cimicidae)

Autoři

SASÍNKOVÁ, Markéta, Jana KŘEMENOVÁ (203 Česká republika, domácí), Petr CHAJMA (203 Česká republika), Tomáš BARTONIČKA (203 Česká republika, domácí), Christian MASSINO (276 Německo), Oliver OTTI (276 Německo) a Ondrej BALVIN (203 Česká republika, garant)

Vydání

Journal of Medical Entomology, Cary, NC, Oxford Univ Press Inc, 2024, 0022-2585

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10613 Zoology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

odkaz na text článku pdf

Impakt faktor

Impact factor: 2.100 v roce 2022

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1093/jme/tjad083

UT WoS

001088393400001

Klíčová slova anglicky

insect rearing; diet; blood-sucking insect; number of eggs; blood preservation

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 27. 2. 2024 08:46, Mgr. Marie Šípková, DiS.

Anotace

V originále

Rearing common bed bugs (Cimex lectularius L.) and other hematophagous insects is essential for basic, medical, and pest-control research. Logistically, acquiring fresh blood can be a challenge, while biologically, the eventual effects of different rearing and blood preparation protocols on bed bug genotype and phenotype pose a risk of biased research results. Using bed bug populations that are either bat- (BL) or human-related (HL), we tested the short- and long-term effects of rearing bugs on live bats or human volunteers, or artificially on CPDA (citrate phosphate dextrose, adenine)-treated blood, measuring meal size, body size, and fertility. We found that artificial feeding did not affect meal size compared with feeding on natural hosts. Long-term rearing across many generations of HL on CPDA-preserved blood led to reduced body size and fertility compared with populations reared on human volunteers. Blood preservatives increased the proportion of sterile eggs even after a single feed. Finally, our results indicated that laboratory reared bed bugs were smaller, regardless of the blood source, than wild bugs. Similar effects of artificial feeding or laboratory rearing alone should be considered in future studies using bed bug cultures to choose an appropriate rearing protocol. With regard to switching between bat and human hosts, HL took smaller meals and BL had lower fertility when fed on bats than when fed on humans. We attribute these results to methodological constrains, specifically the inconsistency of bat feeding, rather than to host specialization. Nevertheless, BL can be easily reared using human blood and artificial feeding systems.

Návaznosti

GC18-08468J, projekt VaV

Název: Role adaptace a fenotypové plasticity spermií v ekologické speciaci

Investor: Grantová agentura ČR, Role adaptace a fenotypové plasticity spermií v ekologické speciaci

Citovat

SASÍNKOVÁ, Markéta, Jana KŘEMENOVÁ, Petr CHAJMA, Tomáš BARTONIČKA, Christian MASSINO, Oliver OTTI a Ondrej BALVIN. Changes in body size and fertility due to artificial and natural feeding of laboratory common bed bugs (Heteroptera: Cimicidae). Journal of Medical Entomology. Cary, NC: Oxford Univ Press Inc, 2024, roč. 61, č. 1, s. 34-45. ISSN 0022-2585. Dostupné z: https://dx.doi.org/10.1093/jme/tjad083.

@article{2336360,
   author = {Sasínková, Markéta and Křemenová, Jana and Chajma, Petr and Bartonička, Tomáš and Massino, Christian and Otti, Oliver and Balvin, Ondrej},
   article_location = {Cary, NC},
   article_number = {1},
   doi = {http://dx.doi.org/10.1093/jme/tjad083},
   keywords = {insect rearing; diet; blood-sucking insect; number of eggs; blood preservation},
   language = {eng},
   issn = {0022-2585},
   journal = {Journal of Medical Entomology},
   title = {Changes in body size and fertility due to artificial and natural feeding of laboratory common bed bugs (Heteroptera: Cimicidae)},
   url = {https://doi.org/10.1093/jme/tjad083},
   volume = {61},
   year = {2024}
}

TY  - JOUR
ID  - 2336360
AU  - Sasínková, Markéta - Křemenová, Jana - Chajma, Petr - Bartonička, Tomáš - Massino, Christian - Otti, Oliver - Balvin, Ondrej
PY  - 2024
TI  - Changes in body size and fertility due to artificial and natural feeding of laboratory common bed bugs (Heteroptera: Cimicidae)
JF  - Journal of Medical Entomology
VL  - 61
IS  - 1
SP  - 34-45
EP  - 34-45
PB  - Oxford Univ Press Inc
SN  - 00222585
KW  - insect rearing
KW  - diet
KW  - blood-sucking insect
KW  - number of eggs
KW  - blood preservation
UR  - https://doi.org/10.1093/jme/tjad083
N2  - Rearing common bed bugs (Cimex lectularius L.) and other hematophagous insects is essential for basic, medical, and pest-control research. Logistically, acquiring fresh blood can be a challenge, while biologically, the eventual effects of different rearing and blood preparation protocols on bed bug genotype and phenotype pose a risk of biased research results. Using bed bug populations that are either bat- (BL) or human-related (HL), we tested the short- and long-term effects of rearing bugs on live bats or human volunteers, or artificially on CPDA (citrate phosphate dextrose, adenine)-treated blood, measuring meal size, body size, and fertility. We found that artificial feeding did not affect meal size compared with feeding on natural hosts. Long-term rearing across many generations of HL on CPDA-preserved blood led to reduced body size and fertility compared with populations reared on human volunteers. Blood preservatives increased the proportion of sterile eggs even after a single feed. Finally, our results indicated that laboratory reared bed bugs were smaller, regardless of the blood source, than wild bugs. Similar effects of artificial feeding or laboratory rearing alone should be considered in future studies using bed bug cultures to choose an appropriate rearing protocol. With regard to switching between bat and human hosts, HL took smaller meals and BL had lower fertility when fed on bats than when fed on humans. We attribute these results to methodological constrains, specifically the inconsistency of bat feeding, rather than to host specialization. Nevertheless, BL can be easily reared using human blood and artificial feeding systems.
ER  -

SASÍNKOVÁ, Markéta, Jana KŘEMENOVÁ, Petr CHAJMA, Tomáš BARTONIČKA, Christian MASSINO, Oliver OTTI a Ondrej BALVIN. Changes in body size and fertility due to artificial and natural feeding of laboratory common bed bugs (Heteroptera: Cimicidae). \textit{Journal of Medical Entomology}. Cary, NC: Oxford Univ Press Inc, 2024, roč.~61, č.~1, s.~34-45. ISSN~0022-2585. Dostupné z: https://dx.doi.org/10.1093/jme/tjad083.

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