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@article{2336866,
author = {Krejci, Jana and Arcidiacono, Orazio Angelo and Čegan, Radim and Radaszkiewicz, Katarzyna Anna and Pacherník, Jiří and Pirk, Jan and Pešl, Martin and Fila, Petr and Bartova, Eva},
article_location = {Praha},
article_number = {4},
doi = {http://dx.doi.org/10.33549/physiolres.935078},
keywords = {mES cells; hES cells; FTO; ALKBH5; Epigenetics; Differentiation; Aging},
language = {eng},
issn = {0862-8408},
journal = {Physiological research},
title = {Cell Differentiation and Aging Lead To Up-Regulation of FTO, While the ALKBH5 Protein Level Was Stable During Aging but Up-Regulated During in vitro-Induced Cardiomyogenesis},
url = {https://www.biomed.cas.cz/physiolres/pdf/2023/72_425.pdf},
volume = {72},
year = {2023}
}
TY - JOUR
ID - 2336866
AU - Krejci, Jana - Arcidiacono, Orazio Angelo - Čegan, Radim - Radaszkiewicz, Katarzyna Anna - Pacherník, Jiří - Pirk, Jan - Pešl, Martin - Fila, Petr - Bartova, Eva
PY - 2023
TI - Cell Differentiation and Aging Lead To Up-Regulation of FTO, While the ALKBH5 Protein Level Was Stable During Aging but Up-Regulated During in vitro-Induced Cardiomyogenesis
JF - Physiological research
VL - 72
IS - 4
SP - 425-444
EP - 425-444
PB - Fyziologický ústav AV ČR
SN - 08628408
KW - mES cells
KW - hES cells
KW - FTO
KW - ALKBH5
KW - Epigenetics
KW - Differentiation
KW - Aging
UR - https://www.biomed.cas.cz/physiolres/pdf/2023/72_425.pdf
N2 - FTO and ALKBH5 proteins are essential erasers of N6-adenosine methylation in RNA. We studied how levels of FTO and ALKBH5 proteins changed during mouse embryonic development, aging, cardiomyogenesis, and neuroectodermal differentiation. We observed that aging in male and female mice was associated with FTO up-regulation in mouse hearts, brains, lungs, and kidneys, while the ALKBH5 level remained stable. FTO and ALKBH5 proteins were up-regulated during experimentally induced cardiomyogenesis, but the level of ALKBH5 protein was not changed when neuroectodermal differentiation was induced. HDAC1 depletion in mouse ES cells caused FTO down-regulation. In these cells, mRNA, carrying information from genes that regulate histone signature, RNA processing, and cell differentiation, was characterized by a reduced level of N6-adenosine methylation in specific gene loci, primarily regulating cell differentiation into neuroectoderm. Together, when we compared both RNA demethylating proteins, the FTO protein level undergoes the most significant changes during cell differentiation and aging. Thus, we conclude that during aging and neuronal differentiation, m6A RNA demethylation is likely regulated by the FTO protein but not via the function of ALKBH5.
ER -
KREJCI, Jana, Orazio Angelo ARCIDIACONO, Radim ČEGAN, Katarzyna Anna RADASZKIEWICZ, Jiří PACHERNÍK, Jan PIRK, Martin PEŠL, Petr FILA a Eva BARTOVA. Cell Differentiation and Aging Lead To Up-Regulation of FTO, While the ALKBH5 Protein Level Was Stable During Aging but Up-Regulated During in vitro-Induced Cardiomyogenesis. \textit{Physiological research}. Praha: Fyziologický ústav AV ČR, 2023, roč.~72, č.~4, s.~425-444. ISSN~0862-8408. Dostupné z: https://dx.doi.org/10.33549/physiolres.935078.
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