Cell Differentiation and Aging Lead To Up-Regulation of FTO,
While the ALKBH5 Protein Level Was Stable During Aging but
Up-Regulated During in vitro-Induced Cardiomyogenesis

J 2023

Cell Differentiation and Aging Lead To Up-Regulation of FTO, While the ALKBH5 Protein Level Was Stable During Aging but Up-Regulated During in vitro-Induced Cardiomyogenesis

KREJCI, Jana, Orazio Angelo ARCIDIACONO, Radim ČEGAN, Katarzyna Anna RADASZKIEWICZ, Jiří PACHERNÍK et. al.

Základní údaje

Originální název

Cell Differentiation and Aging Lead To Up-Regulation of FTO, While the ALKBH5 Protein Level Was Stable During Aging but Up-Regulated During in vitro-Induced Cardiomyogenesis

Autoři

KREJCI, Jana (203 Česká republika), Orazio Angelo ARCIDIACONO (380 Itálie), Radim ČEGAN (203 Česká republika), Katarzyna Anna RADASZKIEWICZ (616 Polsko, domácí), Jiří PACHERNÍK (203 Česká republika, domácí), Jan PIRK (203 Česká republika), Martin PEŠL (203 Česká republika, domácí), Petr FILA (203 Česká republika, domácí) a Eva BARTOVA

Vydání

Physiological research, Praha, Fyziologický ústav AV ČR, 2023, 0862-8408

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30201 Cardiac and Cardiovascular systems

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.100 v roce 2022

Kód RIV

RIV/00216224:14310/23:00132199

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.33549/physiolres.935078

UT WoS

001078316100002

Klíčová slova anglicky

mES cells; hES cells; FTO; ALKBH5; Epigenetics; Differentiation; Aging

Štítky

14110115, 14110513, 14110911, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 3. 2024 10:13, Mgr. Marie Šípková, DiS.

Anotace

V originále

FTO and ALKBH5 proteins are essential erasers of N6-adenosine methylation in RNA. We studied how levels of FTO and ALKBH5 proteins changed during mouse embryonic development, aging, cardiomyogenesis, and neuroectodermal differentiation. We observed that aging in male and female mice was associated with FTO up-regulation in mouse hearts, brains, lungs, and kidneys, while the ALKBH5 level remained stable. FTO and ALKBH5 proteins were up-regulated during experimentally induced cardiomyogenesis, but the level of ALKBH5 protein was not changed when neuroectodermal differentiation was induced. HDAC1 depletion in mouse ES cells caused FTO down-regulation. In these cells, mRNA, carrying information from genes that regulate histone signature, RNA processing, and cell differentiation, was characterized by a reduced level of N6-adenosine methylation in specific gene loci, primarily regulating cell differentiation into neuroectoderm. Together, when we compared both RNA demethylating proteins, the FTO protein level undergoes the most significant changes during cell differentiation and aging. Thus, we conclude that during aging and neuronal differentiation, m6A RNA demethylation is likely regulated by the FTO protein but not via the function of ALKBH5.

Citovat

KREJCI, Jana, Orazio Angelo ARCIDIACONO, Radim ČEGAN, Katarzyna Anna RADASZKIEWICZ, Jiří PACHERNÍK, Jan PIRK, Martin PEŠL, Petr FILA a Eva BARTOVA. Cell Differentiation and Aging Lead To Up-Regulation of FTO, While the ALKBH5 Protein Level Was Stable During Aging but Up-Regulated During in vitro-Induced Cardiomyogenesis. Physiological research. Praha: Fyziologický ústav AV ČR, 2023, roč. 72, č. 4, s. 425-444. ISSN 0862-8408. Dostupné z: https://dx.doi.org/10.33549/physiolres.935078.

@article{2336866,
   author = {Krejci, Jana and Arcidiacono, Orazio Angelo and Čegan, Radim and Radaszkiewicz, Katarzyna Anna and Pacherník, Jiří and Pirk, Jan and Pešl, Martin and Fila, Petr and Bartova, Eva},
   article_location = {Praha},
   article_number = {4},
   doi = {http://dx.doi.org/10.33549/physiolres.935078},
   keywords = {mES cells; hES cells; FTO; ALKBH5; Epigenetics; Differentiation; Aging},
   language = {eng},
   issn = {0862-8408},
   journal = {Physiological research},
   title = {Cell Differentiation and Aging Lead To Up-Regulation of FTO, While the ALKBH5 Protein Level Was Stable During Aging but Up-Regulated During in vitro-Induced Cardiomyogenesis},
   url = {https://www.biomed.cas.cz/physiolres/pdf/2023/72_425.pdf},
   volume = {72},
   year = {2023}
}

TY  - JOUR
ID  - 2336866
AU  - Krejci, Jana - Arcidiacono, Orazio Angelo - Čegan, Radim - Radaszkiewicz, Katarzyna Anna - Pacherník, Jiří - Pirk, Jan - Pešl, Martin - Fila, Petr - Bartova, Eva
PY  - 2023
TI  - Cell Differentiation and Aging Lead To Up-Regulation of FTO, While the ALKBH5 Protein Level Was Stable During Aging but Up-Regulated During in vitro-Induced Cardiomyogenesis
JF  - Physiological research
VL  - 72
IS  - 4
SP  - 425-444
EP  - 425-444
PB  - Fyziologický ústav AV ČR
SN  - 08628408
KW  - mES cells
KW  - hES cells
KW  - FTO
KW  - ALKBH5
KW  - Epigenetics
KW  - Differentiation
KW  - Aging
UR  - https://www.biomed.cas.cz/physiolres/pdf/2023/72_425.pdf
N2  - FTO and ALKBH5 proteins are essential erasers of N6-adenosine methylation in RNA. We studied how levels of FTO and ALKBH5 proteins changed during mouse embryonic development, aging, cardiomyogenesis, and neuroectodermal differentiation. We observed that aging in male and female mice was associated with FTO up-regulation in mouse hearts, brains, lungs, and kidneys, while the ALKBH5 level remained stable. FTO and ALKBH5 proteins were up-regulated during experimentally induced cardiomyogenesis, but the level of ALKBH5 protein was not changed when neuroectodermal differentiation was induced. HDAC1 depletion in mouse ES cells caused FTO down-regulation. In these cells, mRNA, carrying information from genes that regulate histone signature, RNA processing, and cell differentiation, was characterized by a reduced level of N6-adenosine methylation in specific gene loci, primarily regulating cell differentiation into neuroectoderm. Together, when we compared both RNA demethylating proteins, the FTO protein level undergoes the most significant changes during cell differentiation and aging. Thus, we conclude that during aging and neuronal differentiation, m6A RNA demethylation is likely regulated by the FTO protein but not via the function of ALKBH5.
ER  -

KREJCI, Jana, Orazio Angelo ARCIDIACONO, Radim ČEGAN, Katarzyna Anna RADASZKIEWICZ, Jiří PACHERNÍK, Jan PIRK, Martin PEŠL, Petr FILA a Eva BARTOVA. Cell Differentiation and Aging Lead To Up-Regulation of FTO, While the ALKBH5 Protein Level Was Stable During Aging but Up-Regulated During in vitro-Induced Cardiomyogenesis. \textit{Physiological research}. Praha: Fyziologický ústav AV ČR, 2023, roč.~72, č.~4, s.~425-444. ISSN~0862-8408. Dostupné z: https://dx.doi.org/10.33549/physiolres.935078.

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