A new device for online nanoscale sampling and capillary
electrophoresis analysis of plant sap composition

J 2024

A new device for online nanoscale sampling and capillary electrophoresis analysis of plant sap composition

MELICHEROVÁ, Natália, Vít GLOSER, Jan SKALÁK, Martin TRTILEK, Jana LAVICKÁ et. al.

Základní údaje

Originální název

A new device for online nanoscale sampling and capillary electrophoresis analysis of plant sap composition

Autoři

MELICHEROVÁ, Natália (703 Slovensko, domácí), Vít GLOSER (203 Česká republika, domácí), Jan SKALÁK (203 Česká republika, domácí), Martin TRTILEK, Jana LAVICKÁ (203 Česká republika, garant) a František FORET (203 Česká republika)

Vydání

Electrophoresis, Hoboken, Wiley-Blackwell, 2024, 0173-0835

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10609 Biochemical research methods

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.900 v roce 2022

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1002/elps.202300201

UT WoS

001090485100001

Klíčová slova anglicky

capillary electrophoresis; plant sap; sampling; stress; xylem

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 27. 3. 2024 09:19, Mgr. Eva Dubská

Anotace

V originále

In this work, an online sampling of plant xylem sap combined with an efficient (CE)-based method was developed and applied to study the kinetics of changes in the sap composition and to assess plant fitness under stress conditions comprehensively. A laboratory-built CE device was developed to provide online sampling and CE analysis of various ionogenic species in the sap during plant stress response. The rapid online sampling and short CE analysis time allow for real-time monitoring of changes in sap constituents in the living plant during the stress response. The developed device was successfully used to analyze chloride, nitrate, and sulfate ions in the plant xylem during the salt stress or stress caused by nitrate deficiency within short time scales.

Návaznosti

EF16_026/0008446, projekt VaV

Název: Integrace signálu a epigenetické reprogramování pro produktivitu rostlin

Citovat

MELICHEROVÁ, Natália, Vít GLOSER, Jan SKALÁK, Martin TRTILEK, Jana LAVICKÁ a František FORET. A new device for online nanoscale sampling and capillary electrophoresis analysis of plant sap composition. Electrophoresis. Hoboken: Wiley-Blackwell, 2024, roč. 45, 3-4, s. 310-317. ISSN 0173-0835. Dostupné z: https://dx.doi.org/10.1002/elps.202300201.

@article{2339080,
   author = {Melicherová, Natália and Gloser, Vít and Skalák, Jan and Trtilek, Martin and Lavická, Jana and Foret, František},
   article_location = {Hoboken},
   article_number = {3-4},
   doi = {http://dx.doi.org/10.1002/elps.202300201},
   keywords = {capillary electrophoresis; plant sap; sampling; stress; xylem},
   language = {eng},
   issn = {0173-0835},
   journal = {Electrophoresis},
   title = {A new device for online nanoscale sampling and capillary electrophoresis analysis of plant sap composition},
   url = {https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/elps.202300201},
   volume = {45},
   year = {2024}
}

TY  - JOUR
ID  - 2339080
AU  - Melicherová, Natália - Gloser, Vít - Skalák, Jan - Trtilek, Martin - Lavická, Jana - Foret, František
PY  - 2024
TI  - A new device for online nanoscale sampling and capillary electrophoresis analysis of plant sap composition
JF  - Electrophoresis
VL  - 45
IS  - 3-4
SP  - 310-317
EP  - 310-317
PB  - Wiley-Blackwell
SN  - 01730835
KW  - capillary electrophoresis
KW  - plant sap
KW  - sampling
KW  - stress
KW  - xylem
UR  - https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/elps.202300201
N2  - In this work, an online sampling of plant xylem sap combined with an efficient (CE)-based method was developed and applied to study the kinetics of changes in the sap composition and to assess plant fitness under stress conditions comprehensively. A laboratory-built CE device was developed to provide online sampling and CE analysis of various ionogenic species in the sap during plant stress response. The rapid online sampling and short CE analysis time allow for real-time monitoring of changes in sap constituents in the living plant during the stress response. The developed device was successfully used to analyze chloride, nitrate, and sulfate ions in the plant xylem during the salt stress or stress caused by nitrate deficiency within short time scales.
ER  -

MELICHEROVÁ, Natália, Vít GLOSER, Jan SKALÁK, Martin TRTILEK, Jana LAVICKÁ a František FORET. A new device for online nanoscale sampling and capillary electrophoresis analysis of plant sap composition. \textit{Electrophoresis}. Hoboken: Wiley-Blackwell, 2024, roč.~45, 3-4, s.~310-317. ISSN~0173-0835. Dostupné z: https://dx.doi.org/10.1002/elps.202300201.

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