Dynamic release of neuronal extracellular vesicles containing
miR-21a-5p is induced by hypoxia

J 2023

Dynamic release of neuronal extracellular vesicles containing miR-21a-5p is induced by hypoxia

KORVENLAITA, Nea, Mireia GOMEZ-BUDIA, Flavia SCOYNI, Cristiana PISTONO, Luca GIUDICE et. al.

Základní údaje

Originální název

Dynamic release of neuronal extracellular vesicles containing miR-21a-5p is induced by hypoxia

Autoři

KORVENLAITA, Nea (garant), Mireia GOMEZ-BUDIA, Flavia SCOYNI, Cristiana PISTONO, Luca GIUDICE, Shaila EAMEN, Sanna LOPPI, de Sande Ana HERNANDEZ, Benjamin HUREMAGIC, Maria BOUVY-LIIVRAND, Merja HEINANIEMI, Minna U KAIKKONEN, Lesley CHENG, Andrew F HILL, Katja M KANNINEN, Guido W JENSTER, Martin E VAN ROYEN, Laura RAMIRO, Joan MONTANER, Tereza BÁŤKOVÁ (203 Česká republika, domácí), Robert MIKULÍK (203 Česká republika, domácí), Rosalba GIUGNO, Jukka JOLKKONEN, Paula KORHONEN a Tarja MALM

Vydání

Journal of Extracellular Vesicles, HOBOKEN, WILEY, 2023, 2001-3078

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30210 Clinical neurology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 16.000 v roce 2022

Kód RIV

RIV/00216224:14110/23:00132277

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1002/jev2.12297

UT WoS

000906496700001

Klíčová slova anglicky

biomarkers; extracellular vesicle; hypoxia; ischemic stroke; miR-21a-5p; neuron

Štítky

14110127, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 11. 2023 14:34, Mgr. Tereza Miškechová

Anotace

V originále

Hypoxia induces changes in the secretion of extracellular vesicles (EVs) in several non-neuronal cells and pathological conditions. EVs are packed with biomolecules, such as microRNA(miR)-21-5p, which respond to hypoxia. However, the true EV association of miR-21-5p, and its functional or biomarker relevance, are inadequately characterised. Neurons are extremely sensitive cells, and it is not known whether the secretion of neuronal EVs and miR-21-5p are altered upon hypoxia. Here, we characterised the temporal EV secretion profile and cell viability of neurons under hypoxia. Hypoxia induced a rapid increase of miR-21a-5p secretion in the EVs, which preceded the elevation of hypoxia-induced tissue or cellular miR-21a-5p. Prolonged hypoxia induced cell death and the release of morphologically distinct EVs. The EVs protected miR-21a-5p from enzymatic degradation but a remarkable fraction of miR-21a-5p remained fragile and non-EV associated. The increase in miR-21a-5p secretion may have biomarker potential, as high blood levels of miR-21-5p in stroke patients were associated with significant disability at hospital discharge. Our data provides an understanding of the dynamic regulation of EV secretion from neurons under hypoxia and provides a candidate for the prediction of recovery from ischemic stroke.

Citovat

KORVENLAITA, Nea, Mireia GOMEZ-BUDIA, Flavia SCOYNI, Cristiana PISTONO, Luca GIUDICE, Shaila EAMEN, Sanna LOPPI, de Sande Ana HERNANDEZ, Benjamin HUREMAGIC, Maria BOUVY-LIIVRAND, Merja HEINANIEMI, Minna U KAIKKONEN, Lesley CHENG, Andrew F HILL, Katja M KANNINEN, Guido W JENSTER, Martin E VAN ROYEN, Laura RAMIRO, Joan MONTANER, Tereza BÁŤKOVÁ, Robert MIKULÍK, Rosalba GIUGNO, Jukka JOLKKONEN, Paula KORHONEN a Tarja MALM. Dynamic release of neuronal extracellular vesicles containing miR-21a-5p is induced by hypoxia. Journal of Extracellular Vesicles. HOBOKEN: WILEY, 2023, roč. 12, č. 1, s. 1-21. ISSN 2001-3078. Dostupné z: https://dx.doi.org/10.1002/jev2.12297.

@article{2339679,
   author = {Korvenlaita, Nea and GomezandBudia, Mireia and Scoyni, Flavia and Pistono, Cristiana and Giudice, Luca and Eamen, Shaila and Loppi, Sanna and Hernandez, de Sande Ana and Huremagic, Benjamin and BouvyandLiivrand, Maria and Heinaniemi, Merja and Kaikkonen, Minna U and Cheng, Lesley and Hill, Andrew F and Kanninen, Katja M and Jenster, Guido W and van Royen, Martin E and Ramiro, Laura and Montaner, Joan and Báťková, Tereza and Mikulík, Robert and Giugno, Rosalba and Jolkkonen, Jukka and Korhonen, Paula and Malm, Tarja},
   article_location = {HOBOKEN},
   article_number = {1},
   doi = {http://dx.doi.org/10.1002/jev2.12297},
   keywords = {biomarkers; extracellular vesicle; hypoxia; ischemic stroke; miR-21a-5p; neuron},
   language = {eng},
   issn = {2001-3078},
   journal = {Journal of Extracellular Vesicles},
   title = {Dynamic release of neuronal extracellular vesicles containing miR-21a-5p is induced by hypoxia},
   url = {https://onlinelibrary.wiley.com/doi/10.1002/jev2.12297},
   volume = {12},
   year = {2023}
}

TY  - JOUR
ID  - 2339679
AU  - Korvenlaita, Nea - Gomez-Budia, Mireia - Scoyni, Flavia - Pistono, Cristiana - Giudice, Luca - Eamen, Shaila - Loppi, Sanna - Hernandez, de Sande Ana - Huremagic, Benjamin - Bouvy-Liivrand, Maria - Heinaniemi, Merja - Kaikkonen, Minna U - Cheng, Lesley - Hill, Andrew F - Kanninen, Katja M - Jenster, Guido W - van Royen, Martin E - Ramiro, Laura - Montaner, Joan - Báťková, Tereza - Mikulík, Robert - Giugno, Rosalba - Jolkkonen, Jukka - Korhonen, Paula - Malm, Tarja
PY  - 2023
TI  - Dynamic release of neuronal extracellular vesicles containing miR-21a-5p is induced by hypoxia
JF  - Journal of Extracellular Vesicles
VL  - 12
IS  - 1
SP  - 1-21
EP  - 1-21
PB  - WILEY
SN  - 20013078
KW  - biomarkers
KW  - extracellular vesicle
KW  - hypoxia
KW  - ischemic stroke
KW  - miR-21a-5p
KW  - neuron
UR  - https://onlinelibrary.wiley.com/doi/10.1002/jev2.12297
N2  - Hypoxia induces changes in the secretion of extracellular vesicles (EVs) in several non-neuronal cells and pathological conditions. EVs are packed with biomolecules, such as microRNA(miR)-21-5p, which respond to hypoxia. However, the true EV association of miR-21-5p, and its functional or biomarker relevance, are inadequately characterised. Neurons are extremely sensitive cells, and it is not known whether the secretion of neuronal EVs and miR-21-5p are altered upon hypoxia. Here, we characterised the temporal EV secretion profile and cell viability of neurons under hypoxia. Hypoxia induced a rapid increase of miR-21a-5p secretion in the EVs, which preceded the elevation of hypoxia-induced tissue or cellular miR-21a-5p. Prolonged hypoxia induced cell death and the release of morphologically distinct EVs. The EVs protected miR-21a-5p from enzymatic degradation but a remarkable fraction of miR-21a-5p remained fragile and non-EV associated. The increase in miR-21a-5p secretion may have biomarker potential, as high blood levels of miR-21-5p in stroke patients were associated with significant disability at hospital discharge. Our data provides an understanding of the dynamic regulation of EV secretion from neurons under hypoxia and provides a candidate for the prediction of recovery from ischemic stroke.
ER  -

KORVENLAITA, Nea, Mireia GOMEZ-BUDIA, Flavia SCOYNI, Cristiana PISTONO, Luca GIUDICE, Shaila EAMEN, Sanna LOPPI, de Sande Ana HERNANDEZ, Benjamin HUREMAGIC, Maria BOUVY-LIIVRAND, Merja HEINANIEMI, Minna U KAIKKONEN, Lesley CHENG, Andrew F HILL, Katja M KANNINEN, Guido W JENSTER, Martin E VAN ROYEN, Laura RAMIRO, Joan MONTANER, Tereza BÁŤKOVÁ, Robert MIKULÍK, Rosalba GIUGNO, Jukka JOLKKONEN, Paula KORHONEN a Tarja MALM. Dynamic release of neuronal extracellular vesicles containing miR-21a-5p is induced by hypoxia. \textit{Journal of Extracellular Vesicles}. HOBOKEN: WILEY, 2023, roč.~12, č.~1, s.~1-21. ISSN~2001-3078. Dostupné z: https://dx.doi.org/10.1002/jev2.12297.

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