Development of lectin arrays for mapping the glycosylations of
proteins and cells

a 2023

Development of lectin arrays for mapping the glycosylations of proteins and cells

KOMÁREK, Jan, Marek KORSÁK, Lukáš FALTINEK a Michaela WIMMEROVÁ

Základní údaje

Originální název

Development of lectin arrays for mapping the glycosylations of proteins and cells

Název česky

Vývoj lektinových mirkočipů pro mapování glykosylace proteinů a buněk

Autoři

KOMÁREK, Jan (203 Česká republika, domácí), Marek KORSÁK (703 Slovensko, domácí), Lukáš FALTINEK (203 Česká republika, domácí) a Michaela WIMMEROVÁ (203 Česká republika, garant, domácí)

Vydání

XXIInd Meeting of Biochemists and Molecular Biologists, 2023

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10609 Biochemical research methods

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/23:00132291

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-280-0409-5

Klíčová slova česky

lektiny; glykosylace; lektinové čipy

Klíčová slova anglicky

lectins; glycosylation; lectin arrays

Změněno: 16. 11. 2023 09:30, Mgr. Jan Komárek, Ph.D.

Anotace

V originále

Saccharides are essential for life, and in addition to their traditional roles as structural and energy storage components, they play crucial roles in many recognition events, signaling, and communication processes [1]. The glycosylation of cells reflects the biological species, tissue, developmental and physiological state of the organism. Importantly, changes in glycosylation patterns were observed in various tumors, and the aberrant glycosylation is often associated with malignancy potential, tumor immune surveillance, and patients´ prognosis [2]. Lectin arrays are widely used tools for analyzing glycosylation of various samples. They utilize a panel of lectins (saccharide-binding proteins with distinct sugar specificities) immobilized onto a solid surface. Lectin arrays allow for rapid and high-throughput glycomic analysis with minimal sample consumption. They can be used for glycan profiling of purified glycoproteins, cell/tissue lysates, membrane vesicles, complex biological samples, prokaryotic and eukaryotic cells. This talk will present the development of a customized lectin array in the microscope slide format. The microarrays are prepared by non-contact piezo dispensing of lectins onto a functionalized glass slide and their immobilization using various surface chemistries (immobilization on epoxy groups, NHS/EDC coupling). Fluorescence measurement is used to detect the binding of the glycosylated samples. The fabricated lectin arrays will be used, e.g., to map the glycosylation changes at different stages of cell differentiation or under different physiopathological conditions. [1] Zhan X, Dravid G, Ye Z, Hammond H, Shamblott M, Gearhart J, Cheng L. Lancet. 2004 [2] Mazalovska M, Kouokam JC. Biomed Res Int. 2020.

Návaznosti

LM2023042, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CIISB - Česká infrastruktura pro integrativní strukturní biologii

Citovat

KOMÁREK, Jan, Marek KORSÁK, Lukáš FALTINEK a Michaela WIMMEROVÁ. Development of lectin arrays for mapping the glycosylations of proteins and cells. In XXIInd Meeting of Biochemists and Molecular Biologists. 2023. ISBN 978-80-280-0409-5.

@proceedings{2339924,
   author = {Komárek, Jan and Korsák, Marek and Faltinek, Lukáš and Wimmerová, Michaela},
   booktitle = {XXIInd Meeting of Biochemists and Molecular Biologists},
   keywords = {lectins; glycosylation; lectin arrays},
   language = {eng},
   isbn = {978-80-280-0409-5},
   title = {Development of lectin arrays for mapping the glycosylations of proteins and cells},
   year = {2023}
}

TY  - CONF
ID  - 2339924
AU  - Komárek, Jan - Korsák, Marek - Faltinek, Lukáš - Wimmerová, Michaela
PY  - 2023
TI  - Development of lectin arrays for mapping the glycosylations of proteins and cells
SN  - 9788028004095
KW  - lectins
KW  - glycosylation
KW  - lectin arrays
N2  - Saccharides are essential for life, and in addition to their traditional roles as structural and energy storage components, they play crucial roles in many recognition events, signaling, and communication processes [1]. The glycosylation of cells reflects the biological species, tissue, developmental and physiological state of the organism. Importantly, changes in glycosylation patterns were observed in various tumors, and the aberrant glycosylation is often associated with malignancy potential, tumor immune surveillance, and patients´ prognosis [2]. Lectin arrays are widely used tools for analyzing glycosylation of various samples. They utilize a panel of lectins (saccharide-binding proteins with distinct sugar specificities) immobilized onto a solid surface. Lectin arrays allow for rapid and high-throughput glycomic analysis with minimal sample consumption. They can be used for glycan profiling of purified glycoproteins, cell/tissue lysates, membrane vesicles, complex biological samples, prokaryotic and eukaryotic cells. This talk will present the development of a customized lectin array in the microscope slide format. The microarrays are prepared by non-contact piezo dispensing of lectins onto a functionalized glass slide and their immobilization using various surface chemistries (immobilization on epoxy groups, NHS/EDC coupling). Fluorescence measurement is used to detect the binding of the glycosylated samples. The fabricated lectin arrays will be used, e.g., to map the glycosylation changes at different stages of cell differentiation or under different physiopathological conditions. [1] Zhan X, Dravid G, Ye Z, Hammond H, Shamblott M, Gearhart J, Cheng L. Lancet. 2004 [2] Mazalovska M, Kouokam JC. Biomed Res Int. 2020.
ER  -

KOMÁREK, Jan, Marek KORSÁK, Lukáš FALTINEK a Michaela WIMMEROVÁ. Development of lectin arrays for mapping the glycosylations of proteins and cells. In \textit{XXIInd Meeting of Biochemists and Molecular Biologists}. 2023. ISBN~978-80-280-0409-5.

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