Production of lectins for lectin arrays

a 2023

Production of lectins for lectin arrays

ROBOTKOVÁ, Kateřina, Václav PECŮCH, Jan KOMÁREK a Michaela WIMMEROVÁ

Základní údaje

Originální název

Production of lectins for lectin arrays

Název česky

Příprava lektinů pro lektinové čipy

Autoři

ROBOTKOVÁ, Kateřina (203 Česká republika, domácí), Václav PECŮCH (203 Česká republika, domácí), Jan KOMÁREK (203 Česká republika, domácí) a Michaela WIMMEROVÁ (203 Česká republika, garant, domácí)

Vydání

XXIInd Meeting of Biochemists and Molecular Biologists, 2023

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/23:00132297

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-280-0409-5

Klíčová slova česky

Lektiny; lektinové čipy; BanLec; ACG; HPA

Klíčová slova anglicky

Lectins; lectin arrays; BanLec; ACG; HPA

Změněno: 16. 11. 2023 16:14, Bc. Kateřina Robotková

Anotace

V originále

Lectins are proteins that specifically bind carbohydrates and participate in recognition processes such as host-pathogen interaction or in various biological events such as cell proliferation, leukocyte migration, or apoptosis [1]. Lectin microarray is a technology that utilizes a collection of lectins immobilized on a solid support (usually a glass slide). It was developed for high-throughput analysis of glycosylation (e.g., of proteins or cells) and can also serve as a diagnostic tool [2]. This work was focused on cloning and recombinant production of mannose-specific lectin BanLec from banana [3], sialic acid-specific lectin ACG from mushroom Agrocybe cylindracea [4], and GalNAc-specific lectin HPA-I from the Roman snail [5]. In contrast to commercial lectins typically isolated from natural sources, lectins produced in E. coli expression system have several advantages (higher purity, lower costs, absence of glycosylation that might interfere with microarray results). All purified lectins will be used for lectin microarrays. [1] N. Sharon, Journal of Biological Chemistry, 2007 [2] S. Hu a D. T. Wong, Prot. Clin. Appl., 2009 [3] V. L. Koshte et al., Biochemical Journal, 1990 [4] F. Yagi et al., Glycoconjugate Journal, 1997 [5] J.-F. Sanchez et al., Journal of Biological Chemistry, 2006

Citovat

ROBOTKOVÁ, Kateřina, Václav PECŮCH, Jan KOMÁREK a Michaela WIMMEROVÁ. Production of lectins for lectin arrays. In XXIInd Meeting of Biochemists and Molecular Biologists. 2023. ISBN 978-80-280-0409-5.

@proceedings{2340098,
   author = {Robotková, Kateřina and Pecůch, Václav and Komárek, Jan and Wimmerová, Michaela},
   booktitle = {XXIInd Meeting of Biochemists and Molecular Biologists},
   keywords = {Lectins; lectin arrays; BanLec; ACG; HPA},
   language = {eng},
   isbn = {978-80-280-0409-5},
   title = {Production of lectins for lectin arrays},
   year = {2023}
}

TY  - CONF
ID  - 2340098
AU  - Robotková, Kateřina - Pecůch, Václav - Komárek, Jan - Wimmerová, Michaela
PY  - 2023
TI  - Production of lectins for lectin arrays
SN  - 9788028004095
KW  - Lectins
KW  - lectin arrays
KW  - BanLec
KW  - ACG
KW  - HPA
N2  - Lectins are proteins that specifically bind carbohydrates and participate in recognition processes such as host-pathogen interaction or in various biological events such as cell proliferation, leukocyte migration, or apoptosis [1]. Lectin microarray is a technology that utilizes a collection of lectins immobilized on a solid support (usually a glass slide). It was developed for high-throughput analysis of glycosylation (e.g., of proteins or cells) and can also serve as a diagnostic tool [2]. This work was focused on cloning and recombinant production of mannose-specific lectin BanLec from banana [3], sialic acid-specific lectin ACG from mushroom Agrocybe cylindracea [4], and GalNAc-specific lectin HPA-I from the Roman snail [5]. In contrast to commercial lectins typically isolated from natural sources, lectins produced in E. coli expression system have several advantages (higher purity, lower costs, absence of glycosylation that might interfere with microarray results). All purified lectins will be used for lectin microarrays. [1] N. Sharon, Journal of Biological Chemistry, 2007 [2] S. Hu a D. T. Wong, Prot. Clin. Appl., 2009 [3] V. L. Koshte et al., Biochemical Journal, 1990 [4] F. Yagi et al., Glycoconjugate Journal, 1997 [5] J.-F. Sanchez et al., Journal of Biological Chemistry, 2006
ER  -

ROBOTKOVÁ, Kateřina, Václav PECŮCH, Jan KOMÁREK a Michaela WIMMEROVÁ. Production of lectins for lectin arrays. In \textit{XXIInd Meeting of Biochemists and Molecular Biologists}. 2023. ISBN~978-80-280-0409-5.

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